Effects of Bamboo Leaf Flavonoids and Artemisia annua Extract on Milk Performance, Milk Somatic Cell Count and Serum Immune and Antioxidant Related Indexes of Dairy Cows with Subclinical Mastitis

HOU Kun; TONG Jinjin; CHU Kangkang; XIONG Benhai; JIANG Linshu

doi:10.3969/j.issn.1006-267x.2019.09.043

2019 , Vol. 31 >Issue 9: 4286 - 4295

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.043

Effects of Bamboo Leaf Flavonoids and Artemisia annua Extract on Milk Performance, Milk Somatic Cell Count and Serum Immune and Antioxidant Related Indexes of Dairy Cows with Subclinical Mastitis

HOU Kun ,
TONG Jinjin ,
CHU Kangkang ,
XIONG Benhai ,
JIANG Linshu

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1. Beijing Institute of Animal Science and Technology, Beijing Key Laboratory of Cow Nutrition, Beijing 102206, China;
2. Beijing Dairy Cattle Center, Beijing Capital Agribusiness Group, Beijing 100192, China;
3. Beijing Institute of Animal Husbandry and Veterinary Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2019-04-16

Online published: 2019-09-17

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Abstract

This experiment was conducted to investigate the effects of bamboo leaf extract and Artemisia annua extract on milk performance, milk somatic cell count (SCC)and serum immune and antioxidant related indexes of dairy cows with subclinical mastitis. Eighteen Chinese Holstein cows with subclinical mastitis[SCC was (41.31+0.49)×10⁴ cell/mL] with similar days in milk, similar milk yield, body weight of (590±15) kg, 2 to 4 parities were selected and randomly divided into 3 groups with 6 cows per group for feeding experiment. Cows in control group were a total mixed ration (TMR), while cows in bamboo leaf flavones group and Artemisia annua extract group were also fed TMR, and supplementary fed with bamboo leaf flavones (30 g/d) and Artemisia annua extract (60 g/d), respectively. The feeding experiment was conducted for 35 days, including pre-test period of 7 days, feeding period of 14 days and post feeding period of 14 days. Blood and milk samples were collected on day 1, 14, 28 to measure milk SCC, milk composition and serum immune-related factor contents and antioxidant indexes, and the milk yield was recorded. The results showed as follows:1) compared with the control group, the milk yield in bamboo leaf flavones group and Artemisia annua extract group was increased by 8.95% and 13.24% (P>0.05), respectively, the 4% fat corrected milk (FCM) yield and milk protein percentage on day 14 were significantly increased (P<0.05), and the milk SCC on day 14 was significantly decreased (P<0.05). 2) The contents of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum on day 14 in bamboo leaf flavones group and Artemisia annua extract group were significantly lower than those in control group (P<0.05); otherwise, the serum interleukin-2 (IL-2) content in serum in Artemisia annua extract group was significantly higher than that in control group (P<0.05). 3) Compared with the control group, the serum malondialdehyde (MDA) content on day 14 was decreased significantly (P<0.05), and serum superoxide dismutase (SOD) activity on day 14 tended to be increased both in bamboo leaf flavones group and Artemisia annua extract group (P=0.06). In conclusion, both bamboo leaf flavonoids and Artemisia annua extract can improve the mill performance, enhance the immune function and antioxidant capacity of dairy cows, and decrease the milk SCC of dairy cows with subclinical mastitis.

Key words： bamboo leaf flavonoids; Artemisia annua extract; antioxidant capacity; immune function; dairy cows; subclinical mastitis

Cite this article

HOU Kun , TONG Jinjin , CHU Kangkang , XIONG Benhai , JIANG Linshu . Effects of Bamboo Leaf Flavonoids and Artemisia annua Extract on Milk Performance, Milk Somatic Cell Count and Serum Immune and Antioxidant Related Indexes of Dairy Cows with Subclinical Mastitis[J]. Chinese Journal of Animal Nutrition, 2019 , 31(9) : 4286 -4295 . DOI: 10.3969/j.issn.1006-267x.2019.09.043

References

[1] WANG T Y,LI Q,BI K S.Bioactive flavonoids in medicinal plants:structure,activity and biological fate[J].Asian Journal of Pharmaceutical Sciences,2018,13(1):12-23.

[2] 栗明月,焦梦荷,蒋林树,等.竹叶黄酮的生理功能及其应用前景[J].中国农学通报,2018,34(32):144-149.
[3] 栗明月,方洛云,苏汉书,等.竹叶提取物对奶牛泌乳性能、血液常规指标、免疫和抗氧化性能的影响[J].动物营养学报,2019,31(7):3302-3309.
[4] 贾若愚.竹提取物对泌乳后期奶牛生产性能和血液生化指标的影响[D].硕士学位论文.合肥:安徽农业大学,2011.
[5] TSUGAMI Y,MATSUNAGA K,SUZUKI T,et al.Isoflavones and their metabolites influence the milk component synthesis ability of mammary epithelial cells through prolactin/STAT5 signaling[J].Molecular Nutrition & Food Research,2017,61(10):1700156.
[6] NAIR M P,MAHAJAN S,REYNOLDS J L,et al.The flavonoid quercetin inhibits proinflammatory cytokine (tumor necrosis factor alpha) gene expression in normal peripheral blood mononuclear cells via modulation of the NF-κB system[J].Clinical and Vaccine Immunology,2006,13(3):319-328.

[7] KIM W S,CHOI W J,LEE S,et al.Anti-inflammatory,antioxidant and antimicrobial effects of artemisinin extracts from Artemisia annua L.[J].The Korean Journal of Physiology & Pharmacology,2015,19(1):21-27.
[8] HUNT S,YOSHIDA M,DAVIS C E,et al.An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils[J].Journal of Inflammation Research,2015,8:9-14.
[9] SHAKIR L,HUSSAIN M,JAVEED A,et al.Artemisinins and immune system[J].European Journal of Pharmacology,2011,668(1/2):6-14.
[10] MYHRSTAD M C W,CARLSEN H,NORDSTRÖM O,et al.Flavonoids increase the intracellular glutathione level by transactivation of the γ-glutamylcysteine synthetase catalytical subunit promoter[J].Free Radical Biology and Medicine,2002,32(5):386-393.

[11] FERREIRA J F S,LUTHRIA D L,SASAKI T,et al.Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer[J].Molecules,2010,15(5):3135-3170.

[12] 贾可敬.竹叶黄酮提取、纯化及抗氧化活性研究[D].硕士学位论文.长沙:中南林业科技大学,2014.
[13] 栗明月,方洛云,苏汉书,等.竹叶提取物对奶牛瘤胃体外发酵参数及产气量的影响[J].动物营养学报,2019,31(4):1816-1822.
[14] 冯仰廉,周建民,张晓明,等.我国奶牛饲料产奶净能值测算方法的研究[J].中国畜牧杂志,1987(1):8-11.
[15] 方必春,高士宝,戴克宏,等.关于校正不同乳脂率奶量公式的探讨[J].中国奶牛,2003(1):39.
[16] ZHAN J S,LIU M M,SU X S,et al.Effects of alfalfa flavonoids on the production performance,immune system,and ruminal fermentation of dairy cows[J].Asian-Australasian Journal of Animal Sciences,2017,30(10):1416-1424.

[17] INKLER A,GESSNER D K,KOCH C,et al.Effects of a plant product consisting of green tea and curcuma extract on milk production and the expression of hepatic genes involved in endoplasmic stress response and inflammation in dairy cows[J].Archives of Animal Nutrition,2015,69(6):425-441.

[18] 杨建英,王艳玲,郭永国,等.大豆黄酮对奶牛免疫功能和血清及乳中激素水平的影响[J].中国畜牧杂志,2006,42(7):15-17.
[19] 李琮,陈俊宏,刘亚伟,等.大豆黄酮、芒柄花素及其组合对奶牛产奶性能、血浆和乳中激素含量的影响[J].动物营养学报,2017,29(1):309-316.
[20] 占今舜,胡利珍,钟小军,等.苜蓿黄酮对奶牛泌乳性能、血清激素和免疫指标的影响[J].中国农业大学学报,2018,23(12):84-90.
[21] LACASSE P,LOLLIVIER V,BRUCKMAIER R M,et al.Effect of the prolactin-release inhibitor quinagolide on lactating dairy cows[J].Journal of Dairy Science,2011,94(3):1302-1309.

[22] SARACILA M,CRISTE R D,PANAITE T D,et al.Artemisia annua as phytogenic feed additive in the diet of broilers (14-35 days) reared under heat stress (32℃)[J].Brazilian Journal of Poultry Science,2018,20(4):825-832.

[23] SAFARI M,GHASEMI E,ALIKHANI M,et al.Supplementation effects of pomegranate by-products on oxidative status,metabolic profile,and performance in transition dairy cows[J].Journal of Dairy Science,2018,101(12):11297-11309.

[24] BOUVIER-MULLER J,ALLAIN C,ENJALBERT F,et al.Somatic cell count-based selection reduces susceptibility to energy shortage during early lactation in a sheep model[J].Journal of Dairy Science,2018,101(3):2248-2259.

[25] 李睿文,李铁拴,关鸣,等.康贝防治奶牛隐性乳房炎疗效观察及对乳汁体细胞数的影响[J].中国兽医杂志,2002(6):11-13.
[26] CUSHNIE T P T,LAMB A J.Recent advances in understanding the antibacterial properties of flavonoids[J].International Journal of Antimicrobial Agents,2011,38(2):99-107.

[27] DE SANTIS S,KUNDE D,SERINO G,et al.Secretory leukoprotease inhibitor is required for efficient quercetin-mediated suppression of TNFα secretion[J].Oncotarget,2016,7(46):75800-75809.
[28] BURMA? CZUK A,HOLA P,MILCZAK A,et al.Quercetin decrease somatic cells count in mastitis of dairy cows[J].Research in Veterinary Science,2018,117:255-259.
[29] FAN H K,COOK J A.Molecular mechanisms of endotoxin tolerance[J].Journal of Endotoxin Research,2004,10(2):71-84.
[30] KASIMANICKAM R K,KASIMANICKAM V R,OLSEN J R,et al.Associations among serum pro-and anti-inflammatory cytokines,metabolic mediators,body condition,and uterine disease in postpartum dairy cows[J].Reproductive Biology and Endocrinology,2013,11:103.
[31] 朱志宁,郝振荣,王明,等.大豆异黄酮对高产奶牛泌乳后期乳腺肥大细胞分泌肿瘤坏死因子-α和表面型免疫球蛋白A水平的影响[J].动物营养学报,2011,23(1):112-121.
[32] CHO S Y,PARK S J,KWON M J,et al.Quercetin suppresses proinflammatory cytokines production through MAP kinases and NF-κB pathway in lipopolysaccharide-stimulated macrophage[J].Molecular and Cellular Biochemistry,2003,243(1/2):153-160.

[33] SORDILLO L M,PIGHETTI G M,DAVIS M R.Enhanced production of bovine tumor necrosis factor-α during the periparturient period[J].Veterinary Immunology and Immunopathology,1995,49(3):263-270.

[34] FERREIRA V L,BORBA H H L, DE F. BONETTI A,et al.Cytokines and interferons:types and functions[M]//KHAN W A.Autoantibodies and cytokines.Wahid Ali Khan:IntechOpen,2018.
[35] ZECCONI A,PICCININI R,FIORINA S,et al.Evaluation of interleukin-2 treatment for prevention of intramammary infections in cows after calving[J].Comparative Immunology,Microbiology and Infectious Diseases,2009,32(5):439-451.

[36] SACK G H,Jr.Serum amyloid A-a review[J].Molecular Medicine,2018,24(1):46.
[37] KARREMAN H J,WENTINK G H,WENSING T.Using serum amyloid a to screen dairy cows for sub-clinical inflammation[J].Veterinary Quarterly,2000,22(3):175-178.

[38] WANG B,TU Y,ZHAO S P,et al.Effect of tea saponins on milk performance,milk fatty acids,and immune function in dairy cow[J].Journal of Dairy Science,2017,100(10):8043-8052.

[39] 占今舜,詹康,陈小连,等.苜蓿素对脂多糖诱导下奶牛乳腺上皮细胞炎症和乳蛋白合成相关基因表达的影响[D].草业科学,2015,24(12):139-145.
[40] REZAEI A,SHEKARFOROUSH S,CHANGIZI ASHTIYANI S,et al.The effects of Artemisia aucheri extract on hepatotoxicity induced by thioacetamide in male rats[J].Avicenna Journal of Phytomedicine,2013,3(4):293-301.
[41] RAVISHANKAR D,RAJORA A K,GRECO F,et al.Flavonoids as prospective compounds for anti-cancer therapy[J].The International Journal of Biochemistry & Cell Biology,2013,45(12):2821-2831.

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