Study on Serum Metabolomics of Cattle Fed Asparagus Peel or Napiergrass

YU Xia; WANG Qiye; ZHANG Xuelei; YANG Huansheng; GAO Shuxin; WU Duanqin

doi:10.3969/j.issn.1006-267x.2019.09.044

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 9: 4296 - 4302

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.044

Study on Serum Metabolomics of Cattle Fed Asparagus Peel or Napiergrass

YU Xia ,
WANG Qiye ,
ZHANG Xuelei ,
YANG Huansheng ,
GAO Shuxin ,
WU Duanqin

Expand

1. College of Animal Science and Technology, Inner Monglia University for Nationalities, Tongliao 028000, China;
2. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China;
3. School of Life Science, Hunan Normal University, Changsha 410081, China

Received date: 2019-02-16

Online published: 2019-09-17

Fold

Abstract

This study was conducted to investigate the effects of asparagus peel or napiergrass on serum metabolites of cattle based on metabonomics technique. Ten healthy Angus beef cattle[(236.50±34.00) kg of body weight] with similar ages were randomly divided into 2 groups with 5 replicates per group and 1 cattle per replicate. Cattle in the two group were fed the total mixed rations (TMR) with asparagus peel or napiergrass as roughage. The pre-experimental period lasted for 15 days, and the experimental period lasted for 45 days. On the last day of experiment, the blood samples were collected from tail venous before morning feeding, and then serum was isolated, and serum metabolites were detected by gas chromatography-mass spectrometry (GC-MS) technique, using principal component analysis (PCA) showed the distribution of metabolites, screened and identified the metabolites, and analysis the metabolic pathways. The results showed that 16 different metabolites were obtained in two groups. Twelve metabolites of asparagus peel group were up-regulated compared with napiergrass group including biuret, β-glutamic acid, cycloleucine and 2-deoxy-D-glucose, etc, but L-cysteine, cumic acid, N-acetyltryptophan and isoleucine were down-regulated, these metabolites were mainly related to 7 pathways including alanine, aspartate and glutamate metabolism, arginine and proline metabolism, valine, leucine and isoleucine biosynthesis and citrate cycle, etc. In conclusion, under the conditions of this experiment, feeding cattle with asparagus peel is more beneficial to nutrient metabolism than napiergrass.

Key words： asparagus peel; napiergrass; cattle; serum; metabolomics

Cite this article

YU Xia , WANG Qiye , ZHANG Xuelei , YANG Huansheng , GAO Shuxin , WU Duanqin . Study on Serum Metabolomics of Cattle Fed Asparagus Peel or Napiergrass[J]. Chinese Journal of Animal Nutrition, 2019 , 31(9) : 4296 -4302 . DOI: 10.3969/j.issn.1006-267x.2019.09.044

References

[1] LI X X,DURMIC Z,LIU S M,et al.Eremophila glabra reduces methane production and methanogen populations when fermented in a Rusitec[J].Anaerobe,2014,29:100-107.
[2] 武炳超,张欢,童磊,等.象草不同辐射剂量诱变系表型及遗传变异研究[J].草业学报,2018,27(11):77-86.
[3] 王郝为,侯振平,杨焕胜,等.几种非常规粗饲料养分及其饲用价值的比较研究[J].草地学报,2018,26(1):189-194.
[4] 李琴,赵雪燕,杨江科.响应面优化超声辅助提取芦笋黄酮工艺研究[J].武汉轻工大学学报,2016,35(3):39-44.
[5] 冯翠萍,程俊丽,周玉龙,等.芦笋皮提取物抗氧化性研究[J].山西农业大学学报(自然科学版),2011,31(1):57-60.
[6] 王郝为,吴端钦.代谢组学及其在动物营养中的应用[J].动物营养学报,2017,29(12):4301-4307.
[7] 肖英平.早期断奶仔猪血清代谢组学研究及谷氨酰胺对日粮消化吸收和代谢的影响[D].博士学位论文.杭州:浙江大学,2012:53-63.
[8] 中华人民共和国农业部.NY/T 815-2004肉牛饲养标准[S].北京:中国农业出版社,2004.
[9] 国家市场监督管理总局,国家标准化管理委员会.GB/T 6432-2018饲料中粗蛋白的测定凯氏定氮法[S].北京:中国标准出版社,2018.
[10] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6433-2006饲料中粗脂肪的测定[S].北京:中国标准出版社,2006.
[11] 张崇玉,王保哲,张贵国,等.饲料中的粗纤维、NDF、ADF和ADL含量的快速测定方法[J].山东畜牧兽医,2015,36(9):20-22.
[12] KIND T,WOHLGEMUTH G,LEE D Y,et al.FiehnLib:mass spectral and retention index libraries for metabolomics based on quadrupole and time-of-flight gas chromatography/mass spectrometry[J].Analytical Chemistry,2009,81(24):10038-10048.

[13] DUNN W B,BROADHURST D,BEGLEY P,et al.Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry[J].Nature Protocols,2011,6(7):1060-1083.

[14] 王郝为,王启业,吴端钦.刈割高度对象草营养成分及饲用价值的影响分析[J].中国饲料,2018(3):73-75.
[15] 冯德庆,黄勤楼,李春燕,等.28种牧草的脂肪酸组成分析研究[J].草业学报,2011,20(6):214-218.
[16] 冯翠萍,常霞,卢耀环.芦笋皮对实验性高脂症大鼠血脂水平的影响[J].山西农业大学学报,2001,21(3):265-267.
[17] 刘海江.缩二脲饲喂肉牛试验[J].粮食与饲料工业,1997(7):32.
[18] 罗惠娣,毛杨毅,梁茂文,等.缩二脲喂羊试验[J].畜牧与兽医,2005,37(7):62-63.
[19] 秦颖超,周加义,朱敏,等.谷氨酸吸收转运及对肠道发育影响的研究进展[J].动物营养学报,2019,31(2):544-552.
[20] 张建超,韩奇鹏,彭禄庭,等.亮氨酸对细胞影响的研究与应用[J].基因组学与应用生物学,2018,37(8):3550-3556.
[21] GOU L,LEE J,YANG J M,et al.Inhibition of tyrosinase by fumaric acid:integration of inhibition kinetics with computational docking simulations[J].International Journal of Biological Macromolecules,2017,105:1663-1669.
[22] JI Y,WU Z L,DAI Z L,et al.Excessive L-cysteine induces vacuole-like cell death by activating endoplasmic reticulum stress and mitogen-activated protein kinase signaling in intestinal porcine epithelial cells[J].Amino Acids,2016,48(1):149-156.

[23] WILLIAMS B B Jr,VOSS E.A study of the toxicity and local anesthetic properties of some derivatives of cumic acid[J].Journal of the American Pharmaceutical Association,1951,40(9):449-453.

[24] 杨秀娟,杨志军,李硕,等.基于超高效液相色谱-四极杆飞行时间质谱联用技术的血瘀模型大鼠血浆代谢组学分析[J].色谱,2019,37(1):71-79.
[25] 王佳美,刘欣跃.支链氨基酸与2型糖尿病的联系[J].国际检验医学杂志,2019,40(5):590-593.
[26] SUN H Z,WANG D M,WANG B,et al.Metabolomics of four biofluids from dairy cows:potential biomarkers for milk production and quality[J].Journal of Proteome Research,2015,14(2):1287-1298.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References