Analysis of Fecal Microflora Diversity for Thoroughbred Foals before Weaning

LI Xiaobin; LI Hai; LI Fengming; ZHAO Guodong; CHEN Kaixu; MA Yuhui; XU Wenhui; ZANG Changjiang

doi:10.3969/j.issn.1006-267x.2019.09.051

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 9: 4363 - 4370

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.051

Analysis of Fecal Microflora Diversity for Thoroughbred Foals before Weaning

LI Xiaobin ,
LI Hai ,
LI Fengming ,
ZHAO Guodong ,
CHEN Kaixu ,
MA Yuhui ,
XU Wenhui ,
ZANG Changjiang

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1. Xinjiang Key Laboratory of Herbivore Nutrition for Meat & Milk Production, Xingjiang Agricultural University, Urumqi 830052, China;
2. Animal Husbandry and Veterinary Station of Zhaoli County in Yili of Xinjiang, Zhaosu 835600, China

Received date: 2019-03-06

Online published: 2019-09-17

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Abstract

The aim of this study was to investigate the fecal microflora diversity of thoroughbred foals before weaning. Five thoroughbred local self-bred male foals with similar birth date and the average body weight of (128.56±8.34) kg were selected which were numbered CJ1, CJ2, CJ3, CJ4 and CJ5, and fecal samples of foals at 1 week before weaning were collected throughout the day for high-throughput sequencing technique to analyze the diversity of fecal microflora of foals. The results showed as follows:1) at phylum level, the top 10 bacteria in foal feces were Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Euryarchaeota, Verrucomicrobia, Spirochaetes, unidentified_Bacteria, Fibrobacteres and Tenericutes, and the total abundance of Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria was more than 90%. 2) The highest abundance of bacteria in foal feces numbered CJ1 and CJ3 was Proteobacteria, with the abundance of 74.01% and 75.72%, respectively. The highest abundance of bacteria in foal feces numbered CJ2, CJ4 and CJ5 was Firmicutes, with the abundance of 69.75%, 68.27% and 57.85%, respectively. Moraxellaceae and Acinetobacter in foal feces numbered CJ1 and CJ3 were the dominant bacteria in the family and genus levels, respectively, with the abundances of both more than 70%. Thus, the higher abundances of bacteria in fecal microflora of thoroughbred foals are Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria. Due to individual differences, the main species of intestinal bacteria in foals are different. The higher abundances of bacteria in fecal microflora of foals with intestinal inflammation (foals numbered CJ1 and CJ3) were Proteobacteria, Moraxellaceae and Acinetobacter, which are the dominant bacteria at individual classification level.

Key words： foals; pre-weaning; fecal microflora; diversity

Cite this article

LI Xiaobin , LI Hai , LI Fengming , ZHAO Guodong , CHEN Kaixu , MA Yuhui , XU Wenhui , ZANG Changjiang . Analysis of Fecal Microflora Diversity for Thoroughbred Foals before Weaning[J]. Chinese Journal of Animal Nutrition, 2019 , 31(9) : 4363 -4370 . DOI: 10.3969/j.issn.1006-267x.2019.09.051

References

[1] 曾秀玲,熊力,华莹,等.复合益生菌对早期断奶仔猪生长性能和体液免疫水平的影响研究[J].畜牧与饲料科学,2019(2):20-24.
[2] 黄小流,颜小星,张童童,等.马齿苋多糖对早期断奶大鼠生长性能、血清生化指标和肠道形态的影响[J].动物营养学报,2019,31(4):1773-1780.
[3] 张瑞阳,孟玲,李方方,等.包被丁酸钠对断奶仔猪生长性能、血清生化指标、养分表观消化率和粪便微生物菌群的影响[J].动物营养学报,2019,31(5):2296-2302.
[4] 马露,刘文慧,阿拉腾珠拉,等.外源添加短链脂肪酸调控断奶前犊牛胃肠道健康发育潜在功能和机制[J].动物营养学报,2019,31(6):2465-2470.
[5] 李晓斌,赵国栋,刘振,等.3-6月龄伊犁马肠道微生物群落多样性的研究[J].动物营养学报,2017,29(5):1535-1544.
[6] JULLIAND V,GOACHET A G.Fecal microflora as a marker of cecal or colonic microflora in horses[C]//Proceedings of the 19th Equine Science Society Symposium.Tucson,Arizona,2015.
[7] TURNBAUGH P J,HAMADY M,YATSUNENKO T,et al.A core gut microbiome in obese and lean twins[J].Nature,2009,457(7228):480-484.

[8] DOMINGUEZ-BELLO M G,COSTELLO E K,CONTRERAS M,et al.Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(26):11971-11975.

[10] BÄCKHED F,DING H,WANG T,et al.The gut microbiota as an environmental factor that regulates fat storage[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(44):15718-15723.

[10] RUSSELL S L,GOLD M J,WILLING B P,et al.Perinatal antibiotic treatment affects murine microbiota,immune responses and allergic asthma[J].Gut Microbes,2013,4(2):158-164.

[11] FAVIER C F,VAUGHAN E E,DE VOS W M,et al.Molecular monitoring of succession of bacterial communities in human neonates[J].Applied and Environmental Microbiology,2002,68(1):219-226.

[12] HARLOW B E,LAWRENCE L M,FLYTHE M D.Diarrhea-associated pathogens,lactobacilli and cellulolytic bacteria in equine feces:responses to antibiotic challenge[J].Veterinary Microbiology,2013,166(1/2):225-232.
[13] MOREAU M M,EADES S C,REINEMEYER C R,et al.Illumina sequencing of the V4 hypervariable region 16S rRNA gene reveals extensive changes in bacterial communities in the cecum following carbohydrate oral infusion and development of early-stage acute laminitis in the horse[J].Veterinary Microbiology,2014,168(2/3/4):436-441.
[14] KUHL J,WINTERHOFF N,WULF M,et al.Changes in faecal bacteria and metabolic parameters in foals during the first six weeks of life[J].Veterinary Microbiology,2011,151(3/4):321-328.
[15] MONTAGNE L,BOUDRY G,FAVIER C,et al.Main intestinal markers associated with the changes in gut architecture and function in piglets after weaning[J].British Journal of Nutrition,2007,97(1):45-57.

[16] WIJTTEN P J,VAN DER MEULEN J,VERSTEGEN M W.Intestinal barrier function and absorption in pigs after weaning:a review[J].British Journal of Nutrition,2011,105(7):967-981.

[17] MOESER A J,KLOK C V,RYAN K A,et al.Stress signaling pathways activated by weaning mediate intestinal dysfunction in the pig[J].American Journal of Physiology:Gastrointestinal and Liver Physiology2007,292(1):G173-G81.
[18] HU C H,XIAO K,LUAN Z S,et al.Early weaning increases intestinal permeability,alters expression of cytokine and tight junction proteins,and activates mitogen-activated protein kinases in pigs[J].Journal of Animal Science,2013,91(3):1094-1101.

[19] MOESER A J,RYAN K A,NIGHOT P K,et al.Gastrointestinal dysfunction induced by early weaning is attenuated by delayed weaning and mast cell blockade in pigs[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2007,293(2):G413-G421.
[20] SMITH F,CLARK J E,OVERMAN B L,et al.Early weaning stress impairs development of mucosal barrier function in the porcine intestine[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2010,298(3):G352-G363.
[21] MAIN R G,DRITZ S S,TOKACH M D,et al.Increasing weaning age improves pig performance in a multisite production system[J].Journal of Animal Science,2004,82(5):1499-1507.

[22] COSTA M C,STÄMPFLI H R,ALLEN-VERCOE E,et al.Development of the faecal microbiota in foals[J].Equine Veterinary Journal,2016,48(6):681-688.

[23] FLINT H J,BAYER E A.Plant cell wall breakdown by anaerobic microorganisms from the mammalian digestive tract[J].Annals of the New York Academy of Sciences,2008,1125:280-288.
[24] REEVES A E,KOENIGSKNECHT M J,BERGIN I L,et al.Suppression of Clostridium difficile in the gastrointestinal tracts of germfree mice inoculated with a murine isolate from the family Lachnospiraceae[J].Infection and Immunity,2012,80(11):3786-3794.

[25] DERRIEN M,VAUGHAN E E,PLUGGE C M,et al.Akkermansia muciniphila gen. nov.,sp. nov.,a human intestinal mucin-degrading bacterium[J].International Journal of Systematic and Evolutionary Microbiology,2004,54(5):1469-1476.

[26] SUCHODOLSK J S,STEPHENSON C E,BUONO A,et al.Postnatal development of the canine fecal microbiome[M].Red Hook,NY:Curran Associates,Inc.,2013.
[27] COSTA M C,STÄMPFLI H R,ARROYO L G,et al.Changes in the equine fecal microbiota associated with the use of systemic antimicrobial drugs[J].BMC Veterinary Research,2015,11:19.

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