Effects of Two Salinities and Three Sugar Sources on Growth and Glucose Metabolism of Litopenaeus vannamei

LI Xuehe; GUO Ran; JIA Gaowang; ZHANG Yu; XIA Hui; WANG Xiaoming; JING Bingyan

doi:10.3969/j.issn.1006-267x.2019.09.054

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 9: 4387 - 4395

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.054

Effects of Two Salinities and Three Sugar Sources on Growth and Glucose Metabolism of Litopenaeus vannamei

LI Xuehe ,
GUO Ran ,
JIA Gaowang ,
ZHANG Yu ,
XIA Hui ,
WANG Xiaoming ,
JING Bingyan

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College of Oceanography, Hebei Agricultural University, Qinhuangdao 066003, China

Received date: 2019-02-20

Online published: 2019-09-17

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Abstract

In order to study the effects of two salinities and three sugar sources on the growth and glucose metabolism of Litopenaeus vannamei, a total of 720 Litopenaeus vannamei with initial body weight of (0.39±0.01) g were selected. A 3×2 two factors experimental design with 2 salinities of 12‰ and 30‰ and 3 sugar sources of glucose, sucrose and starch was carried out. There were 6 treatments, and every treatment had 3 replicates with 40 Litopenaeus vannamei in each replicate. The feeding experiment lasted for 56 days. The results showed as follows:1) the sugar sources had significant effects on final body weight, specific growth rate and weight gain rate (P<0.05), and glucose group was higher than other two groups; had no significant effects on survival rate and hepatopancreas somatic index (P>0.05); had significant effects on protein efficiency ratio under 30‰ salinity(P<0.05). The salinity had significant effects on final body weight, specific growth rate and weight gain rate in glucose group (P<0.05), and 12‰ salinty was higher than 30‰ salinty. The interaction of salinity and sugar sources had significant effects on final body weight and specific growth rate (P<0.05). 2) The salinity and sugar sources had no significant effects on the hepatic glycogen content (P>0.05), and the interaction of salinity and sugar sources had no significant effects on the hepatic glycogen content (P>0.05). After feeding 4 h, the muscle glycogen content had significant difference between two salinity (P<0.05), and starch group was higher than other groups; before feeding and after feeding 2 and 6 h, the muscle glycogen content had significant difference between two salinity (P<0.05), and 30‰ salinty was higher than 12‰ salinty; the interaction of salinity and sugar sources had no significant effects on the muscle glycogen content (P>0.05). 3) Sugar sources had significant effects on the activities of lipid synthase, malate dehydrogenase, phosphofructokinase, glutamic-pyruvic transaminase and succinate dehydrogenase (P<0.05), and starch group was higher than other groups. While salinity had significant effects on the activities of lipid synthase, phosphofructokinase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase and glutathione transaminase (P<0.05), and 12‰ salinty was higher than 30‰ salinty. The interaction of salinity and sugar sources had significant effects on the activities of lipid synthase and alanine aminotransferase (P<0.05). It is concluded that the sucrose group under 12‰ salinity has the best effect, and has a good promoting effect on Litopenaeus vannamei growth.

Key words： Litopenaeus vannamei; sugar source; salinity; glucose metabolism

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LI Xuehe , GUO Ran , JIA Gaowang , ZHANG Yu , XIA Hui , WANG Xiaoming , JING Bingyan . Effects of Two Salinities and Three Sugar Sources on Growth and Glucose Metabolism of Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 2019 , 31(9) : 4387 -4395 . DOI: 10.3969/j.issn.1006-267x.2019.09.054

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