Effects of N-Carbamylglutamate on Growth Performance, Morphometric Parameters and Plasma Biochemical Parameters of Japanese Seabass (Lateolabrax japonocus)

HUANG Haoyan; YU Huanhuan; LIANG Xiaofang; ZHENG Yinhua; WANG Chunping; WU Xiufeng; CHEN Pei; XUE Min

doi:10.3969/j.issn.1006-267x.2019.10.023

2019 , Vol. 31 >Issue 10: 4590 - 4601

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.023

Effects of N-Carbamylglutamate on Growth Performance, Morphometric Parameters and Plasma Biochemical Parameters of Japanese Seabass (Lateolabrax japonocus)

HUANG Haoyan ,
YU Huanhuan ,
LIANG Xiaofang ,
ZHENG Yinhua ,
WANG Chunping ,
WU Xiufeng ,
CHEN Pei ,
XUE Min

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1. National Aquafeed Safety Assessment Station, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Beijing Animore Science & Technology Co., Ltd., Beijing 100193, China;
3. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2019-03-22

Online published: 2019-10-17

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Abstract

A 10-week growth trial was to determine the effects of different supplemental levels of N-carbamylglutamate (NCG) in the diet on growth performance, morphometric parameters and plasma biochemical parameters of Japanese seabass (Lateolabrax japonocus), and to determine the optimum requirement of NCG for Japanese seabass. Six experimental diets were designed with NCG supplement levels of 0 (N0), 120 (N120), 240 (N240), 360 (N360), 480 (N480) and 720 mg/kg (N720), respectively. Each diet was fed to 6 replicates with 30 Japanese seabass[initial body weight=(11.67±0.02) g] in each replicate. The results showed that no significant differences in final body weight (FBW), weight gain rate (WGR), fat retention ratio (FRR), special growth rate (SGR), viscerasomatic index (VSI) and hepatosomatic index (HSI) were observed among groups (P>0.05). The N240 group had the highest protein retention ratio (PRR) and the lowest abdominal fat percentage (AFP), and the PRR was significantly higher than that in the groups except N360 group (P<0.05), while the AFP was significantly lower than that in the N480 group (P<0.05). Hepatic fat content was the lowest in the N360 group, which was significantly lower than that in the N0 and N120 groups (P<0.05). There were no significant differences in plasma glucose (GLU), total cholesterol (TC), low density lipoprotein cholesterin (LDL-C) contents and aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities (P>0.05). Plasma urea nitrogen (UN) content in the N120 group was significantly lower than that in the N0, N480 and N720 groups (P<0.05). Plasma triglyceride (TG) content in the N120 group was significantly lower than that in the N0 and N360 groups (P<0.05). Plasma high density lipoprotein cholesterin (HDL-C) content in the N240 group was significantly higher than that in the N360 and N720 groups (P<0.05). Plasma nitric oxide (NO) content was the highest in the N360 group which was significantly higher than that in the N480 group (P<0.05). The results indicate that appropriate dietary NCG supplementation can promote the protein retention, decrease the fat accumulation in liver and abdomen, and improve the metabolism of protein and lipid in Japanese seabass. According to the broken-line models, taking PRR and AFP as evaluation indexes, the highest PRR can be obtained when dietary NCG supplemental level is 219.2 mg/kg, and the lowest AFP can be obtained when dietary NCG supplemental level is 214.3 mg/kg. By comprehensive analysis, optimal dietary NCG supplemental level in the diet of Japanese seabass is 214.3 to 219.2 mg/kg.

Key words： Japanese seabass (Lateolabrax japonocus); N-carbamylglutamate; optimal supplemental level; protein retention ratio; abdominal fat percentage

Cite this article

HUANG Haoyan , YU Huanhuan , LIANG Xiaofang , ZHENG Yinhua , WANG Chunping , WU Xiufeng , CHEN Pei , XUE Min . Effects of N-Carbamylglutamate on Growth Performance, Morphometric Parameters and Plasma Biochemical Parameters of Japanese Seabass (Lateolabrax japonocus)[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4590 -4601 . DOI: 10.3969/j.issn.1006-267x.2019.10.023

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