Choline Requirement of Juvenile Pacific White Shrimp (Litopenaeus vannamei)

AN Wenqiang; HE Haolun; DONG Xiaohui; TAN Beiping; YANG Qihui; CHI Shuyan; LIU Hongyu; ZHANG Shuang; YANG Yuanzhi

doi:10.3969/j.issn.1006-267x.2019.10.025

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 10: 4612 - 4621

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.025

Choline Requirement of Juvenile Pacific White Shrimp (Litopenaeus vannamei)

AN Wenqiang ,
HE Haolun ,
DONG Xiaohui ,
TAN Beiping ,
YANG Qihui ,
CHI Shuyan ,
LIU Hongyu ,
ZHANG Shuang ,
YANG Yuanzhi

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1. Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China;
2. Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China;
2. Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang 524000, China

Received date: 2019-03-29

Online published: 2019-10-17

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Abstract

This experiment was aimed to investigate the effects of dietary choline levels on the growth performance, body composition, tissue choline content, serum biochemical indexes and hepatopancreas antioxidant capacity of juvenile Litopenaeus vannamei, in order to determine the optimum requirement of choline of juvenile Litopenaeus vannamei. Choline chloride was added to a basal diet to formulate six experimental diets with the adding levels of 0 (control), 1 000, 2 000, 3 000, 4 000 and 5 000 mg/kg, which contained 9.30, 980.67, 1 985.42, 2 991.63, 3 987.27, and 4 498.69 mg/kg choline, respectively. A total of 960 healthy and strong shrimps with an initial weight of (0.40±0.01) g were selected and divided into 6 groups with 4 replicates per group and 40 shrimps per replicate. Breeding period was 8 weeks. The results showed as follows:1) the dietary choline levels significantly affected the weight gain rate (WGR), specific growth rate (SGR), feed coefficient (FCR) and survival rate (SR) of shrimp (P<0.05); SGR and SR in the 2 991.63 mg/kg group were significantly higher than those in the control group (P<0.05). 2) Dietary choline levels had no significant effect on moisture and crude protein (CP) contents of whole body (P>0.05). The ether extract (EE) content of whole body decreased gradually with the increase of dietary choline levels, and the control group was significantly higher than other groups (P<0.05). 3) The choline content in hepatopancreas and muscle of shrimp increased with the increase of dietary choline levels (980.67 to 3 987.27 mg/kg), which was significantly higher than that of control group (P<0.05). 4) Compared with the control group, the dietary choline levels (1 985.42 to 4 988.69 mg/kg) significantly decreased the serum alanine transaminase (ALT) and glutamic oxalacetic transaminase (AST) activities of shrimp (P<0.05). With the increase of dietary choline levels, the contents of serum triglyceride (TG), total cholesterol (T-CHOL) and total protein (TP) increased firstly and then stabilized. 5) Dietary choline levels had no significant effect on the activity of superoxide dismutase (SOD) of hepatopancreas (P>0.05). The content of malondialdehyde (MDA) reached the minimum in the 4 498.69 mg/kg group, which was significantly lower than that in the control group and 980.67 mg/kg group (P<0.05), which was not significantly different from the other groups (P>0.05). Under the experimental conditions, based on WGR and the quadratic curve analysis, it is concluded that when the dietary lipid levels is 8% and the optimum choline levels is 2 965.97 mg/kg for juvenile Litopenaeus vannamei.

Key words： Litopenaeus vannamei; choline; growth performance; body composition; serum biochemical indexes; antioxidant capacity

Cite this article

AN Wenqiang , HE Haolun , DONG Xiaohui , TAN Beiping , YANG Qihui , CHI Shuyan , LIU Hongyu , ZHANG Shuang , YANG Yuanzhi . Choline Requirement of Juvenile Pacific White Shrimp (Litopenaeus vannamei)[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4612 -4621 . DOI: 10.3969/j.issn.1006-267x.2019.10.025

References

[1] ZEISEL S H.Choline:an important nutrient in brain development,liver function and carcinogenesis[J].Journal of the American College of Nutrition,1992,11(5):473-481.

[2] BLUSZTAJN J K.Choline,a vital amine[J].Science,1998,281(5378):794-795.

[3] TWIBELL R G,BROWN P B.Dietary choline requirement of juvenile yellow perch (Perca flavescens)[J].The Journal of Nutrition,2000,130(1):95-99.

[4] NRC.Nutrient requirements of fish and shrimp[S].Washington,D.C.:National Academies Press,2011.
[5] ARAI S,NOSE T,HASHIMOTO Y.Qualitative requirements of young eels,Anguilla japonica,for water-soluble vitamins and their deficiency symptoms[J].Bull Freshwater Fish Res Lab,1972,22:69-83.
[6] KETOLA H G.Choline metabolism and nutritional requirement of lake trout (Salvelinus namaycush)[J].Journal of Animal Science,1976,43(2):474-477.

[7] 覃笛根,董晓慧,谭北平,等.饲料胆碱含量对斜带石斑鱼生长性能、体成分、肝脏胆碱含量及脂肪代谢酶活性的影响[J].动物营养学报,2015,27(12):3812-3820.
[8] CRAIG S R,GATLIN Ⅲ D M.Growth and body composition of juvenile red drum (Sciaenops ocellatus) fed diets containing lecithin and supplemental choline[J].Aquaculture,1997,151(1/2/3/4):259-267.
[9] 孙公文,王作洲.胆碱的生物功能及科学禁忌[J].中国动物保健,2008(5):79-82.
[10] SOUTHERN L L,BROWN D R,WERNER D D,et al.Excess supplemental choline for swine[J].Journal of Animal Science,1986,62(4):992-996.

[11] MAI K S,XIAO L D,AI Q H,et al.Dietary choline requirement for juvenile cobia,Rachycentron canadum[J].Aquaculture,2009,289(1/2):124-128.
[12] KANAZAWA A,TESHIMA S I,TANAKA N,et al.Nutritional requirements of prawn-Ⅴ:requirements for choline and inositol[J].Memoirs of Faculty of Fisheries Kagoshima University,1976,25(1):47-51.
[13] SHIAU S Y,LO P S.Dietary choline requirement of juvenile grass shrimp (Penaeus monodon)[J].Animal Science,2001,72(3):477-482.

[14] GONG H,LAWRENCE A L,JIANG D H,et al.Effect of dietary phospholipids on the choline requirement of Litopenaeus vannamei juveniles[J].Journal of the World Aquaculture Society,2003,34(3):289-299.

[15] 夏明宏.凡纳滨对虾幼虾对生物素、烟酸、叶酸和胆碱需要量的研究[D].硕士学位论文.宁波:宁波大学,2014.
[16] AOAC.Official methods of analysis[S].Arlington,VA:Association of Official Analytical Chemists,1997.
[17] AUGUSTIN J.Methods of vitamin assay[M].4th ed.New York:Wiley,1985.
[18] 陈承祯.烟酰胺、胆碱对肉仔鸡生长性能及脂肪代谢的影响[D].硕士学位论文.长春:中国人民解放军军需大学,2002.
[19] 闻治国.胆碱对北京鸭生长发育和脂肪代谢的影响及其调控机制[D].博士学位论文.北京:中国农业大学,2015.
[20] 张文旭,王宝维,葛文华,等.饲粮添加胆碱对鹅生长性能、屠宰性能及养分表观利用率的影响[J].动物营养学报,2013,25(4):778-784.
[21] LUO Z,WEI C C,YE H M,et al.Effect of dietary choline levels on growth performance,lipid deposition and metabolism in juvenile yellow catfish Pelteobagrus fulvidraco[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2016,202:1-7.
[22] JIANG G Z,WANG M,LIU W B,et al.Dietary choline requirement for juvenile blunt snout bream,Megalobrama amblycephala[J].Aquaculture Nutrition,2013,19(4):499-505.

[23] WILSON R P,POE W E.Choline nutrition of fingerling channel catfish[J].Aquaculture,1988,68(1):65-71.

[24] MACHLIN L J.Handbook of vitamins[M].New York:Marcel Dekker,1991.
[25] SHIAU S Y,LO P S.Dietary choline requirements of juvenile hybrid tilapia,Oreochromis niloticus×O. aureus[J].The Journal of Nutrition,2000,130(1):100-103.

[26] DUAN Y,ZHU X,HAN D,et al.Dietary choline requirement in slight methionine-deficient diet for juvenile gibel carp (Carassius auratus gibelio)[J].Aquaculture Nutrition,2012,18(6):620-627.

[27] GRIFFIN M E,WILSON K A,WHITE M R,et al.Dietary choline requirement of juvenile hybrid striped bass[J].The Journal of Nutrition,1994,124(9):1685-1689.

[28] ZHANG Z,WILSON R P.Reevaluation of the choline requirement of fingerling channel catfish (Ictalurus punctatus) and determination of the availability of choline in common feed ingredients[J].Aquaculture,1999,180(1/2):89-98.
[29] 周明.饲料中添加胆碱和卵磷脂对大口黑鲈生长,体组成和肝脏的影响[D].硕士学位论文.广州:中山大学,2007.
[30] 朱瑞俊,李小勤,谢骏,等.饲料中添加氯化胆碱对草鱼成鱼生长、脂肪沉积和脂肪代谢酶活性的影响[J].中国水产科学,2010,17(3):527-535.
[31] POSTON H A.Choline requirement of swim-up rainbow trout fry[J].The Progressive Fish-Culturist,1991,53(4):220-223.

[32] 刘伟,文华,周俊,等.氯化胆碱对中华鲟幼鱼生长和生理指标的影响[J].水利渔业,2007,27(3):91-93.
[33] HUNG S S O,BERGE G M,STOREBAKKEN T.Growth and digestibility effects of soya lecithin and choline chloride on juvenile Atlantic salmon[J].Aquaculture Nutrition,1997,3(2):141-144.

[34] 张志强.胭脂鱼幼鱼对饲料维生素E、胆碱和肌醇需要量的研究[D].硕士学位论文.上海:上海海洋大学,2016.
[35] 帅继祥,张利民,王际英,等.星斑川鲽幼鱼胆碱需求量的研究[J].水生生物学报,2011,35(2):365-371.
[36] 谭树华,何典翼,严芳,等.亚硝酸钠对鲫鱼肝脏丙二醛含量和总抗氧化能力的影响[J].农业环境科学学报,2005,24(增刊):21-24.
[37] 曾端,麦康森,艾庆辉.脂肪肝病变大黄鱼肝脏脂肪酸组成、代谢酶活性及抗氧化能力的研究[J].中国海洋大学学报,2008,38(4):542-546.
[38] KOHEN R,NYSKA A.Invited review:oxidation of biological systems:oxidative stress phenomena,antioxidants,redox reactions,and methods for their quantification[J].Toxicologic Pathology,2002,30(6):620-650.

[39] 张定东,李俊怡,王冰柯,等.胆碱对高脂胁迫的团头鲂肝脏抗氧化、组织结构和免疫力的影响[J].水产学报,2017,41(3):438-447.

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