Effects of Clostridium butyricum on Intestinal Barrier Function of Piglets with Two Genetic Backgrounds and Its Molecular Mechanism

LI Haihua; LI Yupeng; WANG Liuyi; WANG Wenjie; QIAO Jiayun

doi:10.3969/j.issn.1006-267x.2019.10.029

2019 , Vol. 31 >Issue 10: 4647 - 4658

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.029

Effects of Clostridium butyricum on Intestinal Barrier Function of Piglets with Two Genetic Backgrounds and Its Molecular Mechanism

LI Haihua ,
LI Yupeng ,
WANG Liuyi ,
WANG Wenjie ,
QIAO Jiayun

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1. College of Animal Science and Animal Medicine, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Institute of Animal Husbandry and Veterinary, Tianjin 300381, China;
3. Tianjin Normal University, Tianjin 300387, China

Received date: 2019-03-05

Online published: 2019-10-17

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Abstract

This study was aimed to investigate the effects of Clostridium butyricum on the health status, intestinal development, intestinal barrier function, serum cytokine contents and mucosal immunity key protein expression of weaned piglets with two genetic backgrounds. A 2×2 factors design was used in this experiment, 12 "Northeastern indigenous×Berkshire" black piglets with similar body weight and (21±2) days of age were selected and divided into 2 groups (control group and experimental group), randomly, and 12 "Duroc×Landrace×Yorkshire" white piglets with similar body weight and (21±2) days of age were selected and divided into 2 groups (control group and experimental group), randomly. Each group was divided into 6 replicates and each pig was fed in single column. Weaned piglets in control groups were fed basal diets, and those in experimental groups were fed the basal diets supplemented with 5×10¹¹ CFU/kg Clostridium butyricum preparation for 14 days after 3 days adaption. The results showed as follows:1) compared with the each control group, the platelet counts of piglets in the two experimental groups were both increased significantly (P<0.05), the villus length to crypt depth ratios in both jejunum and ileum of the two kinds of piglets were increased significantly (P<0.05), the serum D-lactic contents of the two kinds of weaned piglets were decreased significantly (P<0.05), the claudin-1 mRNA relative expression level in jejunum, and the mRNA relative expression levels of claudin-1, zonula occludens-1 (ZO-1) and occludin in ileum of piglets in binary hybridization experimental group were all increased significantly (P<0.05), the mRNA relative expression levels of ZO-1 and occludin in jejnum of piglets in ternary hybridization experimental group were both increased significantly (P<0.05), the mRNA relative expression levels of Toll-like receptor 2 (TLR2), Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 (MyD88) in jejunum mucosa and TLR2, TLR4 and B-cell lymphoma 3 protein (Bcl3) in ileum mucosa of piglets in binary hybridization experimental group were increased significantly (P<0.05), and the mRNA relative expression levels of TLR2 in jejunum mucosa and TLR2, TLR4, MyD88, Toll-interacting protein (Tollip) and Bcl3 in ileum mucosa of piglets in ternary hybridization experimental group were increased significantly (P<0.05). 2) Compared with the ternary hybridization experimental group, the mRNA relative expression levels of claudin-1, TLR4 and MyD88 in jejunum and claudin-1, ZO-1 and occludin in ileum of piglets in binary hybridization experimental group were increased significantly (P<0.05), but the mRNA relative expression levels of TLR2, TLR4, Tollip and Bcl3 in ileum mucosa of piglets in binary across experimental group were decreased significantly (P<0.05). In conclusion, Clostridium butyricum can improve intestinal development of piglets, activate TLR2/4 signaling pathway in intestinal mucosa of two genetic background piglets, promote the expression of key negative feedback factors in the pathway, increase the expression of tight junction proteins, decrease the intestinal mucosal permeability, improve intestinal barrier function, and there are certain differences in the regulation of intestinal barrier function of the two genetic background weaned piglets.

Key words： Clostridium butyricum; binary hybridization; ternary hybridization; weaned piglets; Toll-like receptor 2; Toll-like receptor 4

Cite this article

LI Haihua , LI Yupeng , WANG Liuyi , WANG Wenjie , QIAO Jiayun . Effects of Clostridium butyricum on Intestinal Barrier Function of Piglets with Two Genetic Backgrounds and Its Molecular Mechanism[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4647 -4658 . DOI: 10.3969/j.issn.1006-267x.2019.10.029

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