Protective Effect of Interleukin-1 Receptor Antagonist on Oxidative Damage Induced by 2,4-Dinitrochlorobenzene in Bovine Mammary Epithelial Cells

GUO Yongmei; YAN Sumei; SHI Binlin

doi:10.3969/j.issn.1006-267x.2019.10.030

2019 , Vol. 31 >Issue 10: 4659 - 4666

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.030

Protective Effect of Interleukin-1 Receptor Antagonist on Oxidative Damage Induced by 2,4-Dinitrochlorobenzene in Bovine Mammary Epithelial Cells

GUO Yongmei ,
YAN Sumei ,
SHI Binlin

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Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2019-03-29

Online published: 2019-10-17

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Abstract

This experiment was conducted to investigate the protective effect of interleukin-1 receptor antagonist (IL-1Ra) on oxidative damage induced by 2,4-dinitrochlorobenzene (DNCB) in bovine mammary epithelial cells (BMEC), and determine to the suitable concentration of IL-1Ra inhibiting interleukin-1 (IL-1) activity. A completely randomized trial design was used in this experiment. The third-generation BMEC was randomly divided into 12 groups with 6 replicates per group. BMEC in group 1 (control group) was treated by culture solution with DNCB and IL-1Ra for 6 h; BMEC in groups 2 to 6 (IL-1Ra treatment groups) was treated by culture solution with 1, 5, 10, 20 and 50 μg/L IL-1Ra for 6 h, respectively; BMEC in group 7 (DNCB damage group) was treated by culture solution with 300 μmol/L DNCB for 6 h; BMEC in groups 8 to 12 (IL-1Ra protection groups) was treated by culture solution with 1, 5, 10, 20 and 50 μg/L IL-1Ra+300 μmol/L DNCB for 6 h, respectively. The results showed that compared with the control group, the relative growth rate (RGR) and the activities of selenoprotein[thioredoxin reductase (TrxR) and glutathione peroxidase (GPx)] plus antioxidase[superoxide dismutase (SOD), catalase (CAT)] and total antioxidant capacity (T-AOC)] were significantly reduced after BMEC was exposed to 300 μmol/L DNCB for 6 h (P<0.05). However, the activities of nitric oxide synthase (iNOS) and reactive oxygen species (ROS) as well as the contents of nitric oxide (NO), IL-1, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and malondialdehyde (MDA) showed opposite changes, they were significantly increased (P<0.05). BMEC was simultaneously exposed by 300 μmol/L DNCB and different concentrations of IL-1Ra for 6 h, the RGR plus antioxidase activities of BMEC were enhanced with the increase of IL-1Ra concentration, and the above indexes in 5, 10, 20 and 50 μg/L IL-1Ra protection groups were significantly higher than those in DNCB damage group (P<0.05), with the highest values of RGR as well as the activities of CAT, GPx and TrxR were found at 10 μg/L IL-Ra. Oppositely, IL-1, IL-6, TNF-α and NO contents and iNOS activity were rose up first and then went down with the increase of IL-1Ra concentration, and the above indexes in 5, 10, 20 and 50 μg/L IL-1Ra protection groups were significantly lower than those in DNCB damage group (P<0.05), with better protective effects of IL-1Ra at 5, 10 and 20 μg/L. BMEC was alone exposed by different concentrations of IL-1Ra had no significant effects on the indexes mentioned above with an exception of TNF-α content in 5 μg/L IL-1Ra treatment group (P>0.05). In summary, IL-1Ra can competitively bind with IL-1R to inhabit the activity of IL-1, and then the oxidative damage and inflammatory response of DNCB were suppressed. Under this experimental condition, the suitable concentration of IL-1Ra inhibiting IL-1 activity is 10 μg/L.

Key words： interleukin-1 receptor antagonist; bovine mammary epithelial cells; inflammation parameter; oxidative damage

Cite this article

GUO Yongmei , YAN Sumei , SHI Binlin . Protective Effect of Interleukin-1 Receptor Antagonist on Oxidative Damage Induced by 2,4-Dinitrochlorobenzene in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4659 -4666 . DOI: 10.3969/j.issn.1006-267x.2019.10.030

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