Deoxynivalenol Induces Apoptosis in Piglet Hippocampal Nerve Cells via Mitochondrial Pathway

ZHU Lei; CAO Li; SHEN Maoyu; CHEN Xiaofang; LI Yu; FENG Shibin; WU Jinjie; WANG Xichun

doi:10.3969/j.issn.1006-267x.2019.10.032

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 10: 4675 - 4683

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.032

Deoxynivalenol Induces Apoptosis in Piglet Hippocampal Nerve Cells via Mitochondrial Pathway

ZHU Lei ,
CAO Li ,
SHEN Maoyu ,
CHEN Xiaofang ,
LI Yu ,
FENG Shibin ,
WU Jinjie ,
WANG Xichun

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College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2019-04-02

Online published: 2019-10-17

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Abstract

The aim of this experiment was to investigate the mechanism of neurotoxicity of deoxynivalenol (DON) on piglet hippocampal nerve cells. Piglet hippocampal nerve cells were selected and cultured to logarithmic phase, and exposed to 0, 250, 500, 1 000 ng/mL DON and 1 000 ng/mL DON+20 μmol/L cysteinyl aspartate-specific protease (Caspase)-8 inhibitor (FMK) for 24 h, respectively. After 24 h culture, cell apoptosis ratio, reactive oxygen (ROS) level and mitochondrial membrane potential were determined by flow cytometry, and the main apoptosis genes in mitochondrial pathway including B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), BH3 interacting domain death agonist (Bid), Caspase-2, Caspase-3 and Caspase-9 mRNA expression levels were determined by fluorescence quantitative PCR (qRT-PCR). The results showed as follows:1) compared with the blank group, the cell apoptosis ratio in 250, 500 and 1 000 ng/mL DON groups was significantly increased (P<0.05 or P<0.01), however, compared with the 1 000 ng/mL DON group, the apoptosis ratio in 1 000 ng/mL DON+FMK group was significantly decreased (P<0.01). 2) Compared with the blank group, the ROS level in 250, 500 and 1 000 ng/mL DON groups was significantly increased (P<0.01), while when compared with 1 000 ng/mL DON group, the ROS level in 1 000 ng/mL DON+FMK group was significantly decreased (P<0.01). 3) Compared with the blank group, the mitochondrial membrane potential in 250, 500 and 1 000 ng/mL DON groups was significantly decreased (P<0.01), but when compared with 1 000 ng/mL DON group, the mitochondrial membrane potential in 1 000 ng/mL DON+FMK group was significantly increased (P<0.01). 4) The mRNA expression levels of Bax, Bid, Caspase-2, Caspase-9 and Caspase-3 in 500 and 1 000 ng/mL DON groups were significantly increased (P<0.05 or P<0.01), while the expression level of Bcl-2 mRNA in 500 and 1 000 ng/mL DON groups was significantly decreased when compared with the blank group (P<0.01); however, compared with 1 000 ng/mL DON group, the expression levels of Bax, Bid, Caspase-2, Caspase-9 and Caspase-3 mRNA in 1 000 ng/mL DON+FMK group were significantly decreased (P<0.01), and the expression level of Bcl-2 mRNA in 1 000 ng/mL DON+FMK group was significantly increased (P<0.01). These results suggest that DON induces apoptosis in piglet hippocampal nerve cells through the mitochondrial pathway.

Key words： deoxynivalenol; piglet hippocampal nerve cells; cell apoptosis; mitochondrial pathway

Cite this article

ZHU Lei , CAO Li , SHEN Maoyu , CHEN Xiaofang , LI Yu , FENG Shibin , WU Jinjie , WANG Xichun . Deoxynivalenol Induces Apoptosis in Piglet Hippocampal Nerve Cells via Mitochondrial Pathway[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4675 -4683 . DOI: 10.3969/j.issn.1006-267x.2019.10.032

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