Effects of Nutritional Compound Additives on Growth Performance, Antioxidant Capacity, Liver Function and Toxin Residues of Growing-Finishing Pigs Fed Diets Contaminated with Aflatoxin B1

PU Junning; YUAN Qinghui; CHEN Daiwen; TIAN Gang; HE Jun; ZHENG Ping; MAO Xiangbing; YU Jie; HUANG Zhiqing; LUO Junqiu; LUO Yuheng; YU Bing

doi:10.3969/j.issn.1006-267x.2019.10.034

2019 , Vol. 31 >Issue 10: 4691 - 4700

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.034

Effects of Nutritional Compound Additives on Growth Performance, Antioxidant Capacity, Liver Function and Toxin Residues of Growing-Finishing Pigs Fed Diets Contaminated with Aflatoxin B₁

PU Junning ,
YUAN Qinghui ,
CHEN Daiwen ,
TIAN Gang ,
HE Jun ,
ZHENG Ping ,
MAO Xiangbing ,
YU Jie ,
HUANG Zhiqing ,
LUO Junqiu ,
LUO Yuheng ,
YU Bing

Expand

Key laboratory of Animal Disease-Resistant Nutrition, Sichuan Province, Key laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China

Received date: 2019-03-16

Online published: 2019-10-17

Fold

Abstract

This experiment was conducted to investigate the effects of nutritional compound additives on growth performance, antioxidant capacity, liver function and toxin residues of growing-finishing pigs fed diets contaminated with aflatoxin B₁ (AFB₁). Twenty-eight healthy Duroc×Landrace×Yorkshire growing-finishing pigs with average body weight of (38.06±0.27) kg were randomly assigned to 4 groups with 7 replicates per group and 1 pig per replicate according to similar body weight. The 4 group were control group (basal diet), AFB₁ group (280 μg/kg AFB₁), compound additive group Ⅰ (AFB₁ diet+0.3% antioxidant compound additive) and compound additive group Ⅱ (AFB₁ diet+0.3% intestinal health compound additive), respectively. The experiment lasted for 102 days. The results showed as follows:compared with control group, average daily gain (ADG) at 51 to 75 kg was significantly decreased (P<0.05), plasma total superoxide dismutase (T-SOD) activity was significantly decreased and plasma malondialdehyde (MDA) content was significantly increased at 135 kg (P<0.05), plasma glutamic oxaloacetic transaminase (GOT) activity was significantly increased at 100 kg (P<0.05), and liver index, AFB₁ and aflatoxin M₁ (AFM₁) contents in liver and kidney and AFB₁ content in longissimus dorsi of growing-finishing pigs in AFB₁ group were significantly increased (P<0.05). Compared with AFB₁ group, ADG at 51 to 75 kg was significantly increased (P<0.05), plasma T-SOD activity was significantly increased at 135 kg (P<0.05), plasma MDA content and GOT activity were significantly decreased at 100 kg (P<0.05), and AFM₁ content in kidney and AFB₁ content in longissimus dorsi of growing-finishing pigs in compound additive group Ⅰ were significantly decreased (P<0.05). Compared with AFB₁ group, plasma T-SOD activity was significantly increased at 135 kg (P<0.05), plasma MDA content was significantly decreased at 100 and 135 kg (P<0.05), and plasma GOT activity at 100 kg of growing-finishing pigs in compound additive group Ⅱ was significantly decreased (P<0.05). In conclusion, dietary 280 μg/kg AFB₁ can reduce growth performance and body antioxidant capacity, increase tissue AFB₁ residue, and lead to liver function of growing-finishing pigs damaged. Adding 0.3% antioxidant compound additive can significantly reduce AFB₁ residue in tissue, improve body antioxidant ability, and effectively relieve the adverse effects of AFB₁ on growth performance and liver function of growing-finishing pigs. Adding 0.3% intestinal health compound additive can significantly improve body antioxidant capacity and alleviate AFB₁ damage to liver of growing-finishing pigs.

Key words： nutritional compound additives; aflatoxin B₁; growth performance; liver function; toxin residue; growing-finishing pigs

Cite this article

PU Junning , YUAN Qinghui , CHEN Daiwen , TIAN Gang , HE Jun , ZHENG Ping , MAO Xiangbing , YU Jie , HUANG Zhiqing , LUO Junqiu , LUO Yuheng , YU Bing . Effects of Nutritional Compound Additives on Growth Performance, Antioxidant Capacity, Liver Function and Toxin Residues of Growing-Finishing Pigs Fed Diets Contaminated with Aflatoxin B₁[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4691 -4700 . DOI: 10.3969/j.issn.1006-267x.2019.10.034

References

[1] MOSS M O.Risk assessment for aflatoxins in foodstuffs[J].International Biodeterioration & Biodegradation,2002,50(3/4):137-142.
[2] MISHRA H N,DAS C.A review on biological control and metabolism of aflatoxin[J].Critical Reviews in Food Science and Nutrition,2003,43(3):245-264.

[3] 左瑞雨,王秋文,常娟,等.AFB₁降解剂对肉鸡生长性能、养分代谢及AFB₁在体内残留的影响[J].中国畜牧兽医,2017,44(2):432-439.
[4] ATHERSTONE C,GRACE D,LINDAHL J F,et al.Assessing the impact of aflatoxin consumption on animal health and productivity[J].African Journal of Food,Agriculture,Nutrition and Development,2016,16(3):10949-10966.

[5] 毕小娟,陈代文,余冰,等.黄曲霉毒素B₁对断奶仔猪生长性能, 肝脏组织及肠道健康的影响[J].动物营养学报,2018,30(8):3276-3284.
[6] 高亿清.不同复合添加剂对采食含霉菌毒素饲粮断奶仔猪生长性能和肠道健康的影响[D].硕士学位论文.雅安:四川农业大学,2015.
[7] TAVCAR-KALCHER G,VRTAC K,PESTEVŠEK U,et al.Validation of the procedure for the determination of aflatoxin B₁ in animal liver using immunoaffinity columns and liquid chromatography with postcolumn derivatisation and fluorescence detection[J].Food Control,2007,18(4):333-337.

[8] YUNUS A W,RAZZAZI-FAZELI E,BOHM J.Aflatoxin B₁ in affecting broiler's performance,immunity,and gastrointestinal tract:a review of history and contemporary issues[J].Toxins,2011,3(6):566-590.

[9] 黄能金.自然霉变玉米对断奶仔猪的毒性作用及添加剂的解毒效果研究[D].硕士学位论文.雅安:四川农业大学,2014.
[10] RUSTEMEYER S M,LAMBERSON W R,LEDOUX D R,et al.Effects of dietary aflatoxin on the health and performance of growing barrows[J].Journal of Animal Science,2010,88(11):3624-3630.

[11] NUNES G L,ROBINSON K,KALYNYCH A,et al.Vitamins C and E inhibit O₂^- production in the pig coronary artery[J].Circulation,1997,96(10):3593-3601.

[12] WANG X,SHEN Z,WANG C,et al.Dietary supplementation of selenium yeast enhances the antioxidant capacity and immune response of juvenile Eriocheir sinensis under nitrite stress[J].Fish & Shellfish Immunology,2019,87:22-31.
[13] LYKKESFELDT J,SVENDSEN O.Oxidants and antioxidants in disease:oxidative stress in farm animals[J].The Veterinary Journal,2007,173(3):502-511.

[14] BEDARD L L,MASSEY T E.Aflatoxin B₁-induced DNA damage and its repair[J].Cancer Letters,2006,241(2):174-183.

[15] FU J C,CHEN Q,DU J,et al.Effectiveness of maifanite in reducing the detrimental effects of aflatoxin B₁ on hematology,aflatoxin B₁ residues,and antioxidant enzymes activities of weanling piglets[J].Livestock Science,2013,157(1):218-224.

[16] ALPSOY L,YILDIRIM A,AGAR G.The antioxidant effects of vitamin A,C,and E on aflatoxin B₁-induced oxidative stress in human lymphocytes[J].Toxicology and Industrial Health,2009,25(2):121-127.

[17] DANG S S,ZHANG X,JIA X L,et al.Protective effects of emodin and astragalus polysaccharides on chronic hepatic injury in rats[J].Chinese Medical Journal,2008,121(11):1010-1014.

[18] JEANNOT E,BOORMAN G A,KOSYK O,et al.Increased incidence of aflatoxin B₁-induced liver tumors in hepatitis virus C transgenic mice[J].International Journal of Cancer,2012,130(6):1347-1356.

[19] SHI Y H,XU Z R,FENG J L,et al.Effects of modified montmorillonite nanocomposite on growing/finishing pigs during aflatoxicosis[J].Asian-Australasian Journal of Animal Sciences,2005,18(9):1305-1309.

[20] CREPPY E E.Update of survey,regulation and toxic effects of mycotoxins in Europe[J].Toxicology Letters,2002,127(1/2/3):19-28.
[21] 李娟娟,苏晓鸥.不同吸附剂对黄曲霉毒素B₁吸附效果的研究[J].中国畜牧兽医,2009,36(8):5-10.
[22] HUSSAIN Z,KHAN M Z,KHAN A,et al.Residues of aflatoxin B₁ in broiler meat:effect of age and dietary aflatoxin B₁ levels[J].Food and Chemical Toxicology,2010,48(12):3304-3307.

[23] NEFF G L,EDDS G T.Aflatoxins B₁ and M₁:tissue residues and feed withdrawal profiles in young growing pigs[J].Food and Cosmetics Toxicology,1981,19:739-742.
[24] YILMAZ S,KAYA E,COMAKLI S.Vitamin E (α tocopherol) attenuates toxicity and oxidative stress induced by aflatoxin in rats[J].Advances in Clinical and Experimental Medicine,2017,26(6):907-917.

[25] 王钟翊.纳米硒对肉鸭日粮中黄曲霉毒素B₁解毒效果的研究[D].硕士学位论文.重庆:西南大学,2009.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References