Alleviating Effects of Adding Sophora japonica, Musa nana flower and Chrysanthemum morifolium on Induced Artificial Rumen Acidosis in Vitro

FAN Yaotian; SUN Jie; XIA Guangliang; ZHAO Fangfang; SUN Hua; WANG Hongrong

doi:10.3969/j.issn.1006-267x.2019.10.041

2019 , Vol. 31 >Issue 10: 4757 - 4765

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.041

Alleviating Effects of Adding Sophora japonica, Musa nana flower and Chrysanthemum morifolium on Induced Artificial Rumen Acidosis in Vitro

FAN Yaotian ,
SUN Jie ,
XIA Guangliang ,
ZHAO Fangfang ,
SUN Hua ,
WANG Hongrong

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2019-03-26

Online published: 2019-10-17

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Abstract

The aim of this experiment was to investigate the alleviating effect of adding Sophora japonica, Musa nana flower and Chrysanthemum morifolium on induced artificial rumen acidosis in vitro, and to provide a theoretical basis for the application of alleviating rumen acidosis in production. The experiment used in vitro batch culture to study the effects of adding Sophora japonica, Musa nana flower and Chrysanthemum morifolium on rumen fermentation parameters in substance with high non-fibrous carbohydrates substance[non-fibrous carbohydrates/neutral detergent fiber (NFC/NDF) was 2.30] and conventional substance (NFC/NDF was 1.19), respectively. Single factor design was used in the experiment, the experiment was divided into 4 groups, and each group was repeated 3 times. Three plants were added 10 mg/mL in rumen fluid, and the control group did not add plant powder. Samples were collected on 3, 6, 9 and 12 h cultured in vitro and fermentation parameters were measured. The results showed as follows:1) in conventional substance, the pH at each fermentation stage of Musa nana flower group was higher than that of the control group (P>0.05); in high non-fibrous carbohydrates substance, the pH at each fermentation stage of control group was significantly higher than that of Chrysanthemum morifolium group (P<0.05). 2) In conventional substance, at fermentation 3 and 6 h, the concentration of lactic acid in fermentation fluid in vitro of Musa nana flower group was significantly higher than that of the control group (P<0.05); at fermentation 12 h, the concentration of lactic acid in fermentation fluid in vitro of Sophora japonica group, Musa nana flower group and Chrysanthemum morifolium group was significantly lower than that of the control group (P<0.05). In high non-fibrous carbohydrates substance, at fermentation 3 and 6 h, the concentration of lactic acid in fermentation fluid in vitro of Musa nana flower group was significantly higher than that of the control group (P<0.05); at fermentation 12 h, the concentration of lactic acid in fermentation fluid in vitro of Chrysanthemum morifolium group was significantly lower than that of the control group (P<0.05). 3) At fermentation 12 h, there was no significant difference on concentration of ammonia nitrogen in fermentation fluid in vitro among all groups (P>0.05), but there were differences in the fermentation process. 4) In conventional substance, at fermentation 12 h, the total volatile fatty acids concentration and propionic acid proportion in fermentation fluid in vitro of Sophora japonica group and Chrysanthemum morifolium group were significantly higher than that of control group and Musa nana flower group (P<0.05), and the proportions of acetic acid and butyric acid were significantly lower than that of control group and Musa nana flower group (P<0.05). In high non-fibrous carbohydrates substance, at fermentation 12 h, the concentration of total volatile fatty acids in fermentation fluid in vitro of Chrysanthemum morifolium group were significantly higher than that of control group and Musa nana flower group (P<0.05), and the proportions of acetic acid, propionic acid and butyric acid had no significant difference with the control group (P>0.05). In conclusion, Chrysanthemum morifolium can alleviate the accumulation of lactic acid in rumen, and Musa nana flower can balance the rumen pH, therefore Musa nana flower and Chrysanthemum morifolium have potential ability to alleviate rumen acidosis.

Key words： Sophora japonica; Musa nana flower; Chrysanthemum morifolium; rumen acidosis

Cite this article

FAN Yaotian , SUN Jie , XIA Guangliang , ZHAO Fangfang , SUN Hua , WANG Hongrong . Alleviating Effects of Adding Sophora japonica, Musa nana flower and Chrysanthemum morifolium on Induced Artificial Rumen Acidosis in Vitro[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4757 -4765 . DOI: 10.3969/j.issn.1006-267x.2019.10.041

References

[1] KRAUSE K M,OETZEL G R.Understanding and preventing subacute ruminal acidosis in dairy herds:a review[J].Animal Feed Science and Technology,2006,126(3/4):215-236.
[2] ERDMAN R A.Dietary buffering requirements of the lactating dairy cow:a review[J].Journal of Dairy Science,1988,71(12):3246-3266.

[3] HUTTON P,WHITE C L,DURMIC Z,et al.Eremophila glabra is an Australian plant that reduces lactic acid accumulation in an in vitro glucose challenge designed to simulate lactic acidosis in ruminants[J].Animal,2009,3(9):1254-1263.

[4] 国家药典委员会.978-7-5067-7337-9中华人民共和国药典2015年版.一部[S].北京:中国医药科技出版社,2015:363.
[5] 冯学芝,刘承华,许晓莉.槐花散加减在牛羊病上的应用[J].中兽医学杂志,2007(3):15-16.
[6] HE X,BAI Y,ZHAO Z,et al.Local and traditional uses,phytochemistry,and pharmacology of Sophora japonica L.:a review[J].Journal of Ethnopharmacology,2016,187:160-182.
[7] PARK K W,LEE J E,PARK K M.Diets containing Sophora japonica L.prevent weight gain in high-fat diet-induced obese mice[J].Nutrition Research,2009,29(11):819-824.

[8] JUNG C H,ZHOU S,DING G X,et al.Antihyperglycemic activity of herb extracts on streptozotocin-induced diabetic rats[J].Bioscience,Biotechnology,and Biochemistry,2006,70(10):2556-2559.

[9] 苗明三,李彩荣,陈元朋.槐花总黄酮对大鼠糖尿病模型血清胰岛素、瘦素和C-肽水平的影响[J].中国现代应用药学,2011,28(10):896-898.
[10] 刘生财,赖钟雄.香蕉花蕾食用价值和药用价值的开发与利用[J].中国农业科技导报,2012,14(3):80-84.
[11] NGAMSAENG A,WANAPAT M,KHAMPA S.Evaluation of local tropical plants by in vitro rumen fermentation and their effects on fermentation end-products[J].Pakistan Journal of Nutrition,2006,5(5):414-418.

[12] KANG S,WANAPAT M,CHERDTHORNG A.Effect of banana flower powder supplementation as a rumen buffer on rumen fermentation efficiency and nutrient digestibility in dairy steers fed a high-concentrate diet[J].Animal Feed Science and Technology,2014,196:32-41.
[13] KANG S,WANAPAT M,CHERDTHONG A,et al.Comparison of banana flower powder and sodium bicarbonate supplementation on rumen fermentation and milk production in dairy cows[J].Animal Production Science,2015,56(10):1650-1661.
[14] 张慧芳,许秀萍,孙宗淼.HPLC法同时测定杭菊中6种成分的含量[J].中药材,2014,37(11):2030-2033.
[15] 夏道宗,吕圭源,于新芬,等.杭白菊总黄酮对铅诱导小鼠氧化损伤的拮抗效应研究[J].中国中药杂志,2008,33(23):2803-2808.
[16] YAMAMOTO J,TADAISHI M,YAMANE T,et al.Hot water extracts of edible Chrysanthemum morifolium Ramat.Exert antidiabetic effects in obese diabetic KK-Ay mice[J].Bioscience,Biotechnology,and Biochemistry,2015,79(7):1147-1154.

[17] 潘晓花.硫胺素对SARA状态下奶牛瘤胃发酵及瘤胃菌群结构的影响[D].硕士学位论文.扬州:扬州大学,2013.
[18] MENKE K H,RAAB L,SALEWSKI A,et al.The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro[J].The Journal of Agricultural Science,1979,93(1):217-222.

[19] ERWIN E S,MARCO G J,EMERY E M,et al.Volatile fatty acid analyses of blood and rumen fluid by gas chromatography[J].Journal of Dairy Science,1961,44(9):1768-1771.

[20] BRODERICK G A,KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.

[21] 冯仰廉,卢德勋,陆治年,等.反刍动物营养学[M].北京:科学出版社,2004.
[22] 熊本海,罗清尧,周正奎,等.中国饲料成分及营养价值表(2018年第29版)制定说明[J].中国饲料,2018(21):64-73.
[23] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6435-2014饲料中水分的测定[S].北京:中国标准出版社,2015.
[24] 国家市场监督管理总局,国家标准化管理委员会.GB/T 6432-2018饲料中粗蛋白的测定凯氏定氮法[S].北京:中国标准出版社,2018.
[25] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.

[26] 中华人民共和国国家质量监督检验检疫总局.GB/T 6436-2002饲料中钙的测定[S].北京:中国标准出版社,2002.
[27] 中华人民共和国国家质量监督检验检疫总局.GB/T 6437-2002饲料中总磷的测定分光光度法[S].北京:中国标准出版社,2002.
[28] GOZHO G N,PLAIZIER J C,KRAUSE D O,et al.Subacute ruminal acidosis induces ruminal lipopolysaccharide endotoxin release and triggers an inflammatory response[J].Journal of Dairy Science,2005,88(4):1399-1403.

[29] 范文秀,张玉泉,王泽云,等.洋槐叶和槐花中营养成分分析的研究[J].广东微量元素科学,2004,11(11):51-53.
[30] 金潇潇.部分菊花品种的营养品质评价[D].硕士学位论文.南京:南京农业大学,2008.
[31] 葛永斌,燕傲蕾,徐娟,等.不同产地的4种药用菊花成分差异与其影响因素[J].黄山学院学报,2015,17(3):65-67.
[32] BHASKAR J J,SHOBHA M S,SAMBAIAH K,et al.Beneficial effects of banana (Musa sp.var.elakki bale) flower and pseudostem on hyperglycemia and advanced glycation end-products (AGEs) in streptozotocin-induced diabetic rats[J].Journal of Physiology and Biochemistry,2011,67(3):415-425.

[33] LETTAT A,NOZIÈRE P,SILBERBERG M,et al.Rumen microbial and fermentation characteristics are affected differently by bacterial probiotic supplementation during induced lactic and subacute acidosis in sheep[J].BMC Microbiology,2012,12:142.
[34] 谢占芳,张倩倩,朱凌佳,等.菊花化学成分及药理活性研究进展[J].河南大学学报(医学版),2015,34(4):290-300.
[35] 谢宝昌,东彦新,吉增福.30种中药对瘤胃细菌的影响[J].中兽医医药杂志,1999(6):11-13.
[36] 汪涛.杭菊品质比较及其影响因子研究[D].博士学位论文.南京:南京农业大学,2012.

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