Black Bean Isoflavones Extracted by Trichoderma rimannii Fermentation and Its Effects on Ovarian Early Growth Response Gene-1 Expression of Female Rats

CHU Xiaoli; CONG Sha; KONG Fanxiu; XIAO Yuyang; CHI Xiaoxing

doi:10.3969/j.issn.1006-267x.2019.10.042

2019 , Vol. 31 >Issue 10: 4766 - 4775

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.042

Black Bean Isoflavones Extracted by Trichoderma rimannii Fermentation and Its Effects on Ovarian Early Growth Response Gene-1 Expression of Female Rats

CHU Xiaoli ,
CONG Sha ,
KONG Fanxiu ,
XIAO Yuyang ,
CHI Xiaoxing

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1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163000, China;
2. Key Laboratory of Agricultural Product Processing and Quality Safety in Heilongjiang Province, Daqing 163000, China

Received date: 2019-03-22

Online published: 2019-10-17

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Abstract

The purpose of this study was to investigate the fermentation conditions of black soybean isoflavones extracted by Trichoderma reesei fermentation, and the effects of black soybean isoflavones on expression of early growth response gene-1 (EGR-1) in ovarian of female rats. The single factor test and orthogonal test were used to study the suitable fermentation conditions of black soybean isoflavones extracted by Trichoderma reesei fermentation. Forty young SD female rats at 49days of age with body weight of (200±20) g were selected as the youth group, and forty elderly SD female rats at 12 months of age with body weight of (490±20) g were selected as the elderly group, rats in youth group and elderly group were each divided into 5 groups with 8 rats per group, which were blank group (NC group), positive control group (PC group) and low (L group), medium (M group), high dose black bean isoflavones groups (H group). Rats in L, M and H groups were given 100, 200 and 300 mg/(kg BW·d) black bean isoflavone by gavage, and rats in the PC group were given 0.5 mg/(kg BW·d) diethylstilbestrol by gavage, lasted for 30 days. The results showed that the optimal conditions for the black bean isoflavones extracted by Trichoderma reesei fermentation were fermentation time of 48 h, bottled ratio of 1:10, the ratio of bacterial liquid to soybean powder of 7:1, and the best extraction rate was about 12.06%. After the treatment with black bean isoflavones, compared with the NC group, the expression levels of EGR-1 protein and mRNA in M, H and PC groups were significantly increased (P<0.05 or P<0.01). Compared between youth group and elderly group, the expression level of EGR-1 protein in L and M groups was significantly difference (P<0.01), and the expression levels of EGR-1 gene in L and H groups was significantly difference (P<0.05 or P<0.01). In conclusion, as a phytoestrogens, black bean isoflavones can increase the expression levels of EGR-1 protein and mRNA at a certain dose, and the effect is similar to estrogen.

Key words： black bean isoflavones; Trichoderma reesei; female rats; early growth response gene-1

Cite this article

CHU Xiaoli , CONG Sha , KONG Fanxiu , XIAO Yuyang , CHI Xiaoxing . Black Bean Isoflavones Extracted by Trichoderma rimannii Fermentation and Its Effects on Ovarian Early Growth Response Gene-1 Expression of Female Rats[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4766 -4775 . DOI: 10.3969/j.issn.1006-267x.2019.10.042

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