Screening of Cellulose-Degradation Strains and Condition Optimization of Mixed Strains Fermentation of Pleurotus eryngii Spent Mushroom Substrate

LI Jiateng; YANG Fanti; WANG Shikang; ZHANG Enping

doi:10.3969/j.issn.1006-267x.2019.10.046

2019 , Vol. 31 >Issue 10: 4802 - 4816

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.046

Screening of Cellulose-Degradation Strains and Condition Optimization of Mixed Strains Fermentation of Pleurotus eryngii Spent Mushroom Substrate

LI Jiateng ,
YANG Fanti ,
WANG Shikang ,
ZHANG Enping

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College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China

Received date: 2019-03-29

Online published: 2019-10-17

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Abstract

The purpose of this study was to reduce the cellulose content in Pleurotus eryngii spent mushroom substrate (PESMS) and improve its feeding quality. The cellulose-degradation strains were screened out from PESMS, and mixed with Saccharomyces cerevisiae and Pediococcus acidilactici to ferment PESMS. The 10 factors affecting the fermentation quality of PESMS were screened by Plackett-Burman design (PBD) with the response value of the degradation rate of neutral detergent fiber (NDF). Based on the PBD results, the steepest climbing test (SCT) and Box-Behnken design (BBD) were designed to further optimize the influencing factors. The fermentation model of PESMS was established, and the optimal fermentation conditions were obtained by solving the regression equation. The results showed as follows:1) three cellulose-degradation strains were screened, Bacillus subtilis, Aspergillus niger and Trichoderma reesei. Among them, Bacillus subtilis and Aspergillus niger can be added as feed in the Catalogue of Feed Additives Varieties (2013) published by the Ministry of Agriculture of the People's Republic of China, and were used as follow-up fermentation test strains. 2) Response surface methodology (RSM) was implemented to optimize the fermentation process of PESMS. The optimum fermentation conditions were 1.94:1.00 for the volume ratio of Bacillus subtilis to Aspergillus Niger, 4.7% for the inoculation of cellulose-degradation dominant strains, 10.4% for the addition of bran, 46.1% for the moisture content, and the fermentation time was 8 days. Under these conditions, the degradation rate of NDF predicted by the model was 21.86%, and the degradation rate of NDF obtained by the validation test was 21.08%. There was no significant difference between the model and the predicted values (P>0.05), and the degradation rate of NDF increased by 15.33% compared with that before optimization. 3) Under the optimum fermentation conditions, the nutritive value of PESMS was significantly increased. The dry matter recovery rate (DMR), crude protein, ether extract contents and viable Lactobacillu number of fermentation group were significantly higher than those of raw material group and fermentation group without bacterium liquid (P<0.05), NDF and acid detergent fiber (ADF) contents and pH were significantly lower than those of raw material group and fermentation group without bacterium liquid (P<0.05), and fermentation could significantly reduce the contents of aflatoxin B1 (AFB1) (P<0.05), but had no significant effect on the contents of deoxynivalenol (DON) and zearalenone (ZEN) (P>0.05). The contents of three mycotoxins before and after fermentation were in accordance with the Hygienical Standard for Feeds (GB 13078-2017). The response surface method is used to optimize the fermentation conditions of the cellulose-degradation strains, which can significantly reduce the cellulose content of PESMS, and improve the nutritional value of feeding. The content of mycotoxin before and after fermentation conform to the Hygienical Standard for Feeds.

Key words： Pleurotus eryngii spent mushroom substrate; cellulose-degradation strains; mixed strains fermentation; response surface methodology

Cite this article

LI Jiateng , YANG Fanti , WANG Shikang , ZHANG Enping . Screening of Cellulose-Degradation Strains and Condition Optimization of Mixed Strains Fermentation of Pleurotus eryngii Spent Mushroom Substrate[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4802 -4816 . DOI: 10.3969/j.issn.1006-267x.2019.10.046

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