Effects of Chloroquine Inhibiting Autophagy on Growth Performance and Blood Indexes Change of Piglets Induced by Deoxynivalenol

LIAO Simeng; QI Ming; YAN Jiameng; LI Jianjun; TANG Yulong; TAN Bi'e; YIN Yulong

doi:10.3969/j.issn.1006-267x.2019.10.047

2019 , Vol. 31 >Issue 10: 4817 - 4824

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.047

Effects of Chloroquine Inhibiting Autophagy on Growth Performance and Blood Indexes Change of Piglets Induced by Deoxynivalenol

LIAO Simeng ,
QI Ming ,
YAN Jiameng ,
LI Jianjun ,
TANG Yulong ,
TAN Bi'e ,
YIN Yulong

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1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. University of Chinese Academy of Sciences, Beijing 100008, China

Received date: 2019-04-05

Online published: 2019-10-17

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Abstract

This experiment was conducted to study the effects of chloroquine (CQ) inhibiting autophagy on growth performance, blood physiological and biochemical indexes, and antioxidant ability of piglets fed diets contaminated by deoxynivalenol (DON). Twenty-four healthy "Long×Large" binary crossbred pigs with an initial body weight of (7.10±0.58) kg were randomly divided into 3 groups with 8 replicates per group and one pig per replicate according to a single factor complete randomized trial design. The control group (CON group) fed a basal diet, DON group was fed a DON diet (1.5 mg/kg), and DON+CQ group was fed the DON diet (1.5 mg/kg) and oral chloroquine (10 mg/mL). At the beginning of the experiment, all groups were fed the conventional basal diet, DON+CQ group was oral 8 mL chloroquine, CON and DON groups were oral administration with an equal volume of saline. On the 7 d after the beginning of the experiment, the DON and DON+CQ groups were fed mildew diet for 1 week,and the experiment lasted for 14 days. The results showed as follows:1) compared with CON group, the average daily gain (ADG) and daily feed intake (ADFI) of piglets significantly decreased (P<0.05), and there was no significant difference in ADG and ADFI between DON+CQ group and the other two groups (P>0.05), and feed/gain was significantly lower than that in DON group (P<0.05). 2) Compared with CON group, serum urea nitrogen (UN) content, glutamic-pyruvic transaminase (ALT) and alkaline phosphatase (ALP) activities in DON group significantly increased (P<0.05), and serum UN content and alkaline phosphatase (ALP) activity in DON+CQ group were significantly lower than those in DON group (P<0.05). Compared with CON group, serum growth hormone (GH) and insulin-like growth factor-Ⅰ (IGF-Ⅰ) contents significantly decreased (P<0.05), and GH and IGF-Ⅰ contents were significantly higher than those in DON group (P<0.05). 3) Compared with CON group, the activities of plasma superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in DON group significantly decreased (P<0.05), and malonaldehyde (MDA) content significantly increased (P<0.05). The activities of activities SOD, GST, GSH-Px and T-AOC in DON+CQ group were significantly higher than those in DON group (P<0.05). The results suggest that the inhibition of autophagy by CQ can improve the growth performance of piglets inhibited by DON, the blood biochemical indexes, the antioxidant capacity of the body, and alleviate the toxic effect of DON on weaned piglets to a certain extent.

Key words： deoxynivalenol; piglets; chloroquine; blood biochemical index; antioxidation

Cite this article

LIAO Simeng , QI Ming , YAN Jiameng , LI Jianjun , TANG Yulong , TAN Bi'e , YIN Yulong . Effects of Chloroquine Inhibiting Autophagy on Growth Performance and Blood Indexes Change of Piglets Induced by Deoxynivalenol[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4817 -4824 . DOI: 10.3969/j.issn.1006-267x.2019.10.047

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