Assessment of Enterobacter cloacae Z0206 Biogenic Nanoselenium Safety and Relative Bioavailability

HU Yuhan; LI Xiaoxiao; HUANG Xiangyun; WANG Fengqin; WANG Yizhen; LU Zeqing

doi:10.3969/j.issn.1006-267x.2019.10.048

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 10: 4825 - 4833

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.048

Assessment of Enterobacter cloacae Z0206 Biogenic Nanoselenium Safety and Relative Bioavailability

HU Yuhan ,
LI Xiaoxiao ,
HUANG Xiangyun ,
WANG Fengqin ,
WANG Yizhen ,
LU Zeqing

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Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Key Laboratory of Animal Nutrition and Feed Science in Eastern China of Ministry of Agriculture, National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Institute of Feed Science, Zhejiang University, Hangzhou 310058, China

Received date: 2019-03-29

Online published: 2019-10-17

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Abstract

This study was conducted to evaluate the safety and relative bioavailability of Enterobacter cloacae Z0206 biogenic nanoselenium (BNS). Firstly, using twenty specific pathogen free (SPF) Kunming mouse (half male and half female, 18 to 22 g) as experimental animals, the acute oral toxicity test was used to evaluate the safety of BNS. On this basis, eighty-four 4-week-old Wistar male rats of clean grade were selected and randomly divided into two groups-control group (24 rats) and selenium-deficient group (60 rats). Rats in the selenium-deficient group were fed a selenium-deficient diet (selenium content was 0.02 mg/kg), while rats in the control group was fed a basic diet (selenium content was 0.15 mg/kg) which supplemented with sodium selenite based on the selenium-deficient diet. After feeding 21 days, six rats in each group were randomly sampled, and then the remaining 54 rats in the selenium-deficient group were randomly divided into 3 groups with 6 replicates and 3 rats each replicate, to receive selenium compensation from different sources of selenium for 35 days. Rats in the 3 groups were fed diets supplemented with sodium selenite, yeast selenium and BNS as selenium sources based on the selenium-deficient diet, respectively, the selenium content in the 3 diets were all 0.15 mg/kg. The results showed that BNS's half lethal dose (LD50) for mice>90.46 mg/kg BW (as selenium), which indicated that it had good safety; after 21 days of selenium depletion, the selenium content in liver, kidney and muscle and glutathione peroxidase (GPx) activity in plasma and liver of rats in the selenium-deficient group were significantly reduced compared with control (P<0.01); after 35 d post selenium supplementation, compared with sodium selenite, BNS could significantly increase the average daily gain and the selenium content in renal and muscle (P<0.05), had a tendency of increase GPx activity in plasma and liver (P>0.05), and it slightly better than yeast selenium. Relative to sodium selenite, with the GPx activity in plasma and liver and the selenium content in liver, kidney and muscle as evaluation indexes, the relative bioavailabilities of BNS were 112.2%, 114.6%, 102.0%, 155.3% and 143.2% and the relative bioavailability of yeast selenium were 101.1%, 101.7%, 103.4%, 149.4% and 110.6%, respectively. Collectively, BNS has better safety; compared with sodium selenite, BNS has evident advantages on improving feed intake, selenium deposition in tissues and selenium containing antioxidant enzyme activity of rats, and its relative bioavailability better than sodium selenite and yeast selenium.

Key words： Enterobacter cloacae Z0206 biogenic nanoselenium; relative bioavailability; antioxidant; selenium deposition; safety

Cite this article

HU Yuhan , LI Xiaoxiao , HUANG Xiangyun , WANG Fengqin , WANG Yizhen , LU Zeqing . Assessment of Enterobacter cloacae Z0206 Biogenic Nanoselenium Safety and Relative Bioavailability[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4825 -4833 . DOI: 10.3969/j.issn.1006-267x.2019.10.048

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