Effects of Dietary Protein Level on Growth Performance, Body Composition and Digestive Enzyme Activities of Juvenile Hybrid of Culter alburnus (♀)&times;Megalobrama terminalis (♂)

CHEN Jianming; HUANG Aixia; TIAN Ruping; SHUN Lihui; JIANG Jianhu; SHEN Binqian; GUO Jianlin; MI Guoqiang

doi:10.3969/j.issn.1006-267x.2019.10.050

2019 , Vol. 31 >Issue 10: 4843 - 4851

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.050

Effects of Dietary Protein Level on Growth Performance, Body Composition and Digestive Enzyme Activities of Juvenile Hybrid of Culter alburnus (♀)×Megalobrama terminalis (♂)

CHEN Jianming ,
HUANG Aixia ,
TIAN Ruping ,
SHUN Lihui ,
JIANG Jianhu ,
SHEN Binqian ,
GUO Jianlin ,
MI Guoqiang

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Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China

Received date: 2019-03-22

Online published: 2019-10-17

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Abstract

This experiment was carried out to evaluate the effects of dietary protein level on growth performance, body composition and digestive enzyme activities of juvenile hybrid of Culter alburnus (♀)×Megalobrama terminalis (♂), in order to determine the optimal dietary protein level. Six iso-energetic practical experimental diets with protein levels of 33% (D33), 36% (D36), 39% (D39), 42% (D42), 45% (D45) and 48% (D48) were fed to 6 groups (each group had 3 replicates and each replicate had 60 fish) of fish (initial average body weight of 1.22 g) for 8 weeks. The results showed as follows:dietary protein level did not significantly affect the survival rate (P>0.05). Weight gain rate and feed efficiency were significantly increased with dietary protein level increased from 33% to 42% (P<0.05), but was not changed significantly with further increasing dietary protein level (P>0.05). Whole body moisture content in D45 and D48 groups were significantly higher than that in other groups (P<0.05), and there was a negative correlation between whole body crude lipid content (y_L) and dietary protein level (x) (y_L=-0.15x+15.78, R²=0.654, P<0.001). Condition factor in D33 group was significantly lower than that in other groups (P<0.05). The vicera-somatic index tended to decrease with the dietary protein level increasing. Dietary protein level did not significantly affect the liver protease activity (P>0.05). Gut protease activity was constantly increased with dietary protein level increasing, but when dietary protein level exceeded 42%, the gut protease activity had no significant change (P>0.05). Liver amylase activity was firstly increased and the decreased with dietary protein level increase, and the highest value was found in D42 group. Gut amylase activity (y_A) negatively correlated with dietary protein level (x) (y_A=-1.73x+99.53, R²=0.997, P<0.001). In conclusion, under the conditions of this experiment, dietary protein level affects the digestive enzyme activities and feed utilization, thus affects the growth performance and body composition. The optimal dietary protein level for juvenile hybrid of Culter alburnus (♀)×Megalobrama terminalis (♂) is estimated to be 42.9% to 43.8% of dry diet by broken-line regression analysis with weight gain rate and feed efficiency as the criteria.

Key words： juvenile hybrid of Culter alburnus (♀)×Megalobrama terminalis (♂); dietary protein level; weight gain rate; feed efficiency; body composition; digestive enzyme activities

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CHEN Jianming , HUANG Aixia , TIAN Ruping , SHUN Lihui , JIANG Jianhu , SHEN Binqian , GUO Jianlin , MI Guoqiang . Effects of Dietary Protein Level on Growth Performance, Body Composition and Digestive Enzyme Activities of Juvenile Hybrid of Culter alburnus (♀)×Megalobrama terminalis (♂)[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4843 -4851 . DOI: 10.3969/j.issn.1006-267x.2019.10.050

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