Effects of Rutin on Growth Performance, Body Composition, Non-Specific Immunity and Intestinal Digestive Enzyme Activities of Grass Carp (Ctenopharyngodon idella)

CHEN Mi; FU Guoxiang; DONG Juane; HAO Wenfang

doi:10.3969/j.issn.1006-267x.2019.10.053

Abstract

The aim of this experiment was to study the effects of rutin on growth performance, body composition, non-specific immunity and intestinal digestive enzyme activities of grass carp (Ctenopharyngodon idella). A total of 135 healthy grass carp with an average body weight of (5.10±0.17) g were selected and randomly divided into 3 groups with 3 replicates per group and 15 grass carp per replicate. Three kinds of experimental diets containing effective rutin at 0 (control), 97.4 (low-dose) and 388.4 mg/kg (high-dose) were fed grass carp in the 3 groups for 56 days, respectively. The results showed as follows:1) compared with the control group, added rutin into the diet could reduce hepatosomatic index (HSI) and increase intestosomatic index (ISI) of grass carp, and the HSI of grass carp in high-dose group and ISI of grass carp in low-dose group had significant differences (P<0.05); otherwise, added rutin into the diet could significantly reduce viscerosomatic index (VSI) of grass carp (P<0.05). 2) Compared with the control group, added rutin into the diet could increase the whole-body crude protein content of grass carp, and it in low-dose group had significant difference (P<0.05). 3) Compared with the control group, added rutin into the diet could significantly increase the liver lysozyme (LZM) activity (P<0.05), and could significantly decrease the liver malondialdehyde (MDA) content of grass carp (P<0.05); added low-dose of rutin into the diet could significantly increase the liver superoxide dismutase (SOD) activity of grass carp (P<0.05); added high-dose of rutin into the diet could significantly increase the liver catalase (CAT) and alkaline phosphatase (AKP) activities of grass carp (P<0.05); added rutin into the diet could increase the serum LZM activity, and there was a dose-effect relationship between them, it in high-dose group was significantly increased (P<0.05); low-dose of rutin had a certain promotive effect on serum AKP activity, but high-dose of rutin had an inhibition effect on serum AKP activity of grass carp, and it in high-dose group was significantly higher than that in control group and low-dose group (P<0.05). 4) Compared with the control group, added rutin into the diet could increase the intestinal amylase (AMS) activity, and it in low-dose group had significant difference (P<0.05); high-dose of rutin had an inhibition effect on intestinal trypsin (PRS) activity, and it in high-dose group was significantly decreased (P<0.05). In summary, rutin has influences on non-specific immunity and intestinal digestion and absorption of grass carp. Based on growth performance, taking into account the indices of body composition, non-specific immunity and intestinal digestive enzyme activities, rutin is suitable for low dose in the diet of grass carp.

Key words： rutin; grass carp; growth performance; body composition; non-specific immunity; intestinal digestive enzyme activities

Cite this article

CHEN Mi , FU Guoxiang , DONG Juane , HAO Wenfang . Effects of Rutin on Growth Performance, Body Composition, Non-Specific Immunity and Intestinal Digestive Enzyme Activities of Grass Carp (Ctenopharyngodon idella)[J]. Chinese Journal of Animal Nutrition, 2019 , 31(10) : 4868 -4876 . DOI: 10.3969/j.issn.1006-267x.2019.10.053

References

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[10] 郑尧,赵志祥,史磊磊,等.芦丁对吉富罗非鱼幼鱼组织切片及细胞凋亡的影响[J].淡水渔业,2017,47(3):73-78.
[11] BAE S H,KIM B R,KANG B J,et al.Molecular cloning of prophenoloxidase and the effects of dietary β-glucan and rutin on immune response in hemocytes of the fleshy shrimp,Fenneropenaeus chinensis[J].Fish & Shellfish Immunology,2012,33(3):597-604.

[12] 余祖功,夏德全,吴婷婷.大豆黄酮对奥利亚罗非鱼生长及相关激素水平、血液生化指标的影响[J].中国兽医学报,2006,26(2):183-185.
[13] SHIN H S,YOO J H,MIN T S,et al.Effect of quercetin on the activity and mRNA expression of antioxidant enzymes and physiological responses in olive flounder (Paralichthys olivaceus) exposed to cadmium[J].Asian-Australasian Journal of Animal Sciences,2010,23(6):742-749.

[14] 杨继华,陈冰,黄燕华,等.饲料中添加桑叶黄酮对吉富罗非鱼生长性能、体成分、抗氧化指标及抗亚硝酸盐应激能力的影响[J].动物营养学报,2017,29(9):3403-3412.
[15] 张青红.饲料中添加杜仲叶粉对青鱼生长、免疫和肉质的影响研究[D].硕士学位论文.南宁:广西大学,2015:12-15.
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[18] 胡海滨,刘金桃,李彦先,等.饲料中大豆黄酮对大菱鲆生长、消化酶活力、抗氧化力及肠道结构的影响[J].水产学报,2014,38(9):1503-1513.
[19] 苏俊锋,郭长江,韦京豫,等.不同肠段对槲皮素、芦丁吸收的比较研究[J].卫生研究,2002,31(1):55-57.
[20] 田丽霞,刘永坚,冯健,等.不同种类淀粉对草鱼生长、肠系膜脂肪沉积和鱼体组成的影响[J].水产学报,2002,26(3):247-251.
[21] 萧培珍.日粮中添加水飞蓟素对草鱼脂质代谢的影响及其机制研究[D].博士学位论文.杨凌:西北农林科技大学,2017:24-53.
[22] 郭旭东,刁其玉,屠焰,等.芦丁对奶牛泌乳性能和血清指标的影响[J].中国农业科学,2010,43(24):5137-5146.
[23] 刘淑兰.不同水平槐属植物黄酮对罗非鱼生长及肝胰脏抗氧化能力的影响[D].硕士学位论文.厦门:集美大学,2012:9-21.
[24] MUONA M,SOIVIO A.Changes in plasma lysozyme and blood leucocyte levels of hatchery-reared Atlantic salmon (Salmo salar L.) and sea trout (Salmo trutta L.) during parr-smolt transformation[J].Aquaculture,1992,106(1):75-87.

[25] 郭旭东,刁其玉,郭宇廷,等.芦丁对大鼠免疫器官指数的影响[J].动物医学进展,2011,32(11):70-72.
[26] HSIEH T J,WANG J C,HU C Y,et al.Effects of Rutin from Toona sinensis on the immune and physiological responses of white shrimp (Litopenaeus vannamei) under Vibrio alginolyticus challenge[J].Fish & Shellfish Immunology,2008,25(5):581-588.

[27] 郑尧,赵志祥,范立民,等.饲料添加芦丁对吉富罗非鱼血清炎症因子的影响[J].中国农学通报,2017,33(26):141-145.
[28] 李军涛,刘寒,解宜兴,等.酒糟酵母培养物对草鱼生长性能和溶菌酶活性的影响[J].中国农学通报,2018,34(5):139-146.
[29] 朱站英.草鱼免疫细胞和免疫相关基因研究:自然杀伤细胞杀伤活性和溶菌酶mRNA的表达[D].硕士学位论文.上海:上海海洋大学,2012:18-25.
[30] 吴锦绣,张胤,李梅,等.芦丁对血清白蛋白构象的影响[J].光谱学与光谱分析,2008,28(11):2619-2622.
[31] 王斯慧,黄琬凌,陈庆松,等.芦丁、槲皮素对α-淀粉酶抑制活性研究[J].食品与发酵科技,2012,48(3):34-37.
[32] 胡小松,辛力,肖华志.苦荞麦浸提液对胰蛋白酶和淀粉酶抑制作用的研究[J].中国食品学报,2003,3(4):62-66.
[33] 邓静,贾旭,杨娟,等.芦丁与胰蛋白酶相互作用的光谱性质研究[J].天然产物研究与开发,2006,18(6):968-971.
[34] 郭旭东.芦丁对奶牛泌乳性能、瘤胃消化代谢和对大鼠乳腺发育的影响[D].博士学位论文.北京:中国农业科学院,2011:83-102.
[1] 陈长春,戴丽红,王琳琳,等.中草药添加剂在畜禽生产应用中的研究进展[J].中国饲料,2018(22):14-17.
[2] 袁天翊,方莲花,吕扬,等.杜仲叶的药理作用研究进展[J].中国中药杂志,2013,38(6):781-785.
[3] 张帅男,李煦照.杜仲化学成分及药理作用研究进展[J].中国民族民间医药,2017,26(10):56-61.
[4] 孙文通.杜仲活性成分对草鱼生长、肌肉品质和胶原蛋白基因表达的影响[D].博士学位论文.上海:上海海洋大学,2017:12-64.
[5] ZHAI S W,LIU S L.Effects of dietary quercetin on growth performance,serum lipids level and body composition of tilapia (Oreochromis niloticus)[J].Italian Journal of Animal Science,2013,12(4):e85.
[6] PAN P H,LIN S H,WANG Y Y,et al.Protective effects of rutin on liver injury induced by biliary obstruction in rats[J].Free Radical Biology and Medicine,2014,73:106-116.
[7] KHAN R A,KHAN M R,SAHREEN S.CCl₄-induced hepatotoxicity:protective effect of rutin on p53,CYP2E1 and the antioxidative status in rat[J].BMC Complementary and Alternative Medicine,2012,12:178.
[8] 李聪伟,李倩,郭兰,等.芦丁对1型糖尿病小鼠肝功能及肝组织形态学的影响[J].四川大学学报(医学版),2018,49(3):384-387,424.
[9] PÊS T S,SACCOL E M H,OURIQUE G M,et al.Effect of diets enriched with rutin on blood parameters,oxidative biomarkers and pituitary hormone expression in silver catfish (Rhamdia quelen)[J].Fish Physiology and Biochemistry,2016,42(1):321-333.

[10] 郑尧,赵志祥,史磊磊,等.芦丁对吉富罗非鱼幼鱼组织切片及细胞凋亡的影响[J].淡水渔业,2017,47(3):73-78.
[11] BAE S H,KIM B R,KANG B J,et al.Molecular cloning of prophenoloxidase and the effects of dietary β-glucan and rutin on immune response in hemocytes of the fleshy shrimp,Fenneropenaeus chinensis[J].Fish & Shellfish Immunology,2012,33(3):597-604.

[12] 余祖功,夏德全,吴婷婷.大豆黄酮对奥利亚罗非鱼生长及相关激素水平、血液生化指标的影响[J].中国兽医学报,2006,26(2):183-185.
[13] SHIN H S,YOO J H,MIN T S,et al.Effect of quercetin on the activity and mRNA expression of antioxidant enzymes and physiological responses in olive flounder (Paralichthys olivaceus) exposed to cadmium[J].Asian-Australasian Journal of Animal Sciences,2010,23(6):742-749.

[14] 杨继华,陈冰,黄燕华,等.饲料中添加桑叶黄酮对吉富罗非鱼生长性能、体成分、抗氧化指标及抗亚硝酸盐应激能力的影响[J].动物营养学报,2017,29(9):3403-3412.
[15] 张青红.饲料中添加杜仲叶粉对青鱼生长、免疫和肉质的影响研究[D].硕士学位论文.南宁:广西大学,2015:12-15.
[16] 王微.替代抗菌促生长抗生素的六种中草药提取物[J].饲料与畜牧,2013(5):39-43.
[17] 陈银银.苜蓿黄酮对扬州鹅生产性能、血液生化指标和免疫性能的影响[D].硕士学位论文.扬州:扬州大学,2017:9-23.
[18] 胡海滨,刘金桃,李彦先,等.饲料中大豆黄酮对大菱鲆生长、消化酶活力、抗氧化力及肠道结构的影响[J].水产学报,2014,38(9):1503-1513.
[19] 苏俊锋,郭长江,韦京豫,等.不同肠段对槲皮素、芦丁吸收的比较研究[J].卫生研究,2002,31(1):55-57.
[20] 田丽霞,刘永坚,冯健,等.不同种类淀粉对草鱼生长、肠系膜脂肪沉积和鱼体组成的影响[J].水产学报,2002,26(3):247-251.
[21] 萧培珍.日粮中添加水飞蓟素对草鱼脂质代谢的影响及其机制研究[D].博士学位论文.杨凌:西北农林科技大学,2017:24-53.
[22] 郭旭东,刁其玉,屠焰,等.芦丁对奶牛泌乳性能和血清指标的影响[J].中国农业科学,2010,43(24):5137-5146.
[23] 刘淑兰.不同水平槐属植物黄酮对罗非鱼生长及肝胰脏抗氧化能力的影响[D].硕士学位论文.厦门:集美大学,2012:9-21.
[24] MUONA M,SOIVIO A.Changes in plasma lysozyme and blood leucocyte levels of hatchery-reared Atlantic salmon (Salmo salar L.) and sea trout (Salmo trutta L.) during parr-smolt transformation[J].Aquaculture,1992,106(1):75-87.

[25] 郭旭东,刁其玉,郭宇廷,等.芦丁对大鼠免疫器官指数的影响[J].动物医学进展,2011,32(11):70-72.
[26] HSIEH T J,WANG J C,HU C Y,et al.Effects of Rutin from Toona sinensis on the immune and physiological responses of white shrimp (Litopenaeus vannamei) under Vibrio alginolyticus challenge[J].Fish & Shellfish Immunology,2008,25(5):581-588.

[27] 郑尧,赵志祥,范立民,等.饲料添加芦丁对吉富罗非鱼血清炎症因子的影响[J].中国农学通报,2017,33(26):141-145.
[28] 李军涛,刘寒,解宜兴,等.酒糟酵母培养物对草鱼生长性能和溶菌酶活性的影响[J].中国农学通报,2018,34(5):139-146.
[29] 朱站英.草鱼免疫细胞和免疫相关基因研究:自然杀伤细胞杀伤活性和溶菌酶mRNA的表达[D].硕士学位论文.上海:上海海洋大学,2012:18-25.
[30] 吴锦绣,张胤,李梅,等.芦丁对血清白蛋白构象的影响[J].光谱学与光谱分析,2008,28(11):2619-2622.
[31] 王斯慧,黄琬凌,陈庆松,等.芦丁、槲皮素对α-淀粉酶抑制活性研究[J].食品与发酵科技,2012,48(3):34-37.
[32] 胡小松,辛力,肖华志.苦荞麦浸提液对胰蛋白酶和淀粉酶抑制作用的研究[J].中国食品学报,2003,3(4):62-66.
[33] 邓静,贾旭,杨娟,等.芦丁与胰蛋白酶相互作用的光谱性质研究[J].天然产物研究与开发,2006,18(6):968-971.
[34] 郭旭东.芦丁对奶牛泌乳性能、瘤胃消化代谢和对大鼠乳腺发育的影响[D].博士学位论文.北京:中国农业科学院,2011:83-102.

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