Diversity of Rumen Microflora in Dairy Buffaloes at Different Ages

PENG Kelan; REN Daxi; LIU Jianxin

doi:10.3969/j.issn.1006-267x.2019.11.020

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 11: 5053 - 5064

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.020

Diversity of Rumen Microflora in Dairy Buffaloes at Different Ages

PENG Kelan ,
REN Daxi ,
LIU Jianxin

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Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Received date: 2019-04-16

Online published: 2019-11-19

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Abstract

This experiment was conducted to study the diversity of rumen microflora in dairy buffaloes at different ages using 16S rRNA high throughput sequencing, and to explore the charge with age increasing. Four age groups were group Y (12 months of age, parity=0), group M (3 to 5 years of age, parity=1), group E (6 to 8 years of age, parity=2), and group O (more than 9 years of age, parity ≥ 3), and had 9, 7, 4 and 5 dairy buffaloes in groups, respectively. Total bacterial DNA was extracted from the rumen fluids. After PCR amplication, V3 to V4 regions of 16S rDNA were sequenced by high-throughput sequencing technology. The results showed as follows:alpha diversity analysis indicated that the number of rumen bacteria tended to decrease with age increasing (P=0.07), and the relative abundance decreased significantly (P<0.05). Beta diversity analysis indicated that significant differences were found in the rumen bacterial structure among dairy buffalo at different ages. At the phylum level, the dominant bacteria for all ages were Bacteroidetes, Firmicutes and Proteobacteria. At the phylum level, the relative abundance of Prevotella_1 was the highest among the four age groups. The relative abundance of dominant and sub-dominant genus tended to close with each other with age increasing. There existed some significant characteristic rumen bacteria at species level among different ages. The Lactobacillus and Saccharofermentans were the main bacterial genus in rumen of dairy buffalo at 12 months of age, and the relative abundance decreased significantly with age increasing (P<0.05); the unclassified_o_Clostridiales and g_norank_f_Bifidobacteriaceae were the main bacterial genus in rumen of dairy buffalo at 3 to 5 years of age, and the relative abundance were significantly higher than those of dairy buffalo at 12 months of age and 9 years of age and above (P<0.05); the relative abundance of g_unclassified_f_Prevotellaceae and g_Candidatus_Saccharimonas were higher than those at 12 months of age and 3 to 5 days of age. Co-occurrence analysis indicated that the correlation and compactness of the rumen bacteria in dairy buffalo were gradually decreased with age increasing. From the results obtained in this work, it is indicated that the rumen bacteria number and relative abundance in dairy buffalo decrease with age increasing. There exist some characteristic rumen bacteria at different ages. The correlation among the rumen bacteria decreases gradually with age increasing, suggesting that the stability of rumen microflora may reduce with the increasing age of dairy buffalo.

Key words： dairy buffalo; rumen; bacterial diversity; bacteria; age

Cite this article

PENG Kelan , REN Daxi , LIU Jianxin . Diversity of Rumen Microflora in Dairy Buffaloes at Different Ages[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5053 -5064 . DOI: 10.3969/j.issn.1006-267x.2019.11.020

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