Effects of High-Lipid Diet Supplemented with Bile Acid on Growth Performance, Muscle Nutritional Component Contents, Digestive Enzyme Activities and Serum Biochemical Indices of Juvenile Schizothorax prenanti

XIANG Xiao; ZENG Benhe; YANG Lixiang; ZHOU Xinghua; CHEN Jian; WANG Wenjuan

doi:10.3969/j.issn.1006-267x.2019.11.024

2019 , Vol. 31 >Issue 11: 5089 - 5099

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.024

Effects of High-Lipid Diet Supplemented with Bile Acid on Growth Performance, Muscle Nutritional Component Contents, Digestive Enzyme Activities and Serum Biochemical Indices of Juvenile Schizothorax prenanti

XIANG Xiao ,
ZENG Benhe ,
YANG Lixiang ,
ZHOU Xinghua ,
CHEN Jian ,
WANG Wenjuan

Expand

1. Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, College of Animal Science of Southwest University, Chongqing 402460, China;
2. Tibet Academy of Agricultural and Animal Husbandry Sciences, Fisheries Research Institute, Lasa 850000, China

Received date: 2019-04-19

Online published: 2019-11-19

Fold

Abstract

This experiment was conducted to study the effects of effects of high-lipid diet supplemented with bile acid on growth performance, muscle nutritional component contents, digestive enzyme activities and serum biochemical indices of juvenile Schizothorax prenanti. A total of 360 juvenile Schizothorax prenanti with an average body weight of (12.74±0.14) g were randomly divided into 4 groups with 3 replicates per group and 30 fish per replicate. The high lipid diets with different bile acid supplemental levels (0, 75, 150 and 300 mg/kg) were fed to juvenile Schizothorax prenanti for 70 days. The results showed that the weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio (PER) initially increased and then decreased with bile acid supplemental level raising. Conversely, the feed conversation ratio (FCR) firstly decreased and then increased with bile acid supplemental level raising. The WGR, SGR and PER were all the highest (263.52%, 1.84%/d and 2.09%, respectively) when the bile acid supplemental level was 150 mg/kg, and those in 150 mg/kg bile acid group were significantly higher than those in other groups (P<0.05); at the same time, the FCR was the lowest (1.23), and it in 150 mg/kg bile acid group was significantly lower than that in control group and 75 mg/kg bile acid group (P<0.05). With bile acid supplemental level raising, the condition factor (CF), hepatosomatic index (HSI)and viscerosomatic index (VSI) were initially increased and then to stabilization, and those in 150 and 300 mg/kg bile acid groups were significantly lower than those in control group and 75 mg/kg bile acid group (P<0.05). The crude protein content in muscle was initially increased and then to stabilization with bile acid supplemental level raising, while the crude lipid content in muscle was initially decreased and then leveled off. The muscle crude protein content in 150 and 300 mg/kg bile acid groups was significantly higher than that in control group and 75 mg/kg bile acid group (P<0.05), and the muscle crude lipid content in 75, 150 and 300 mg/kg bile acid groups was significantly lower than that in control group (P<0.05). There were no significant differences in moisture and crude ash contents in muscle among all groups (P>0.05). With bile acid supplemental level raising, the protease activity in hepatopancreas and the lipase activity in hepatopancreas and foregut initially increased and then decreased, at the same time, the protease activity in foregut and midgut, the lipase activity in midgut was increased gradually. Bile acid supplemental level had no significant effects on the protease and lipase activities in hindgut and the amylase activity in foregut, midgut, hindgut and hepatopancreas (P>0.05). With bile acid level raising, the serum cholesterol (CHO) and triglyceride (TG)contents increased gradually, at the same time, while the serum low density lipoprotein cholesterol (LDL-C) content decreased gradually, and there were significant differences in above indices among all groups (P<0.05). The results of above indicate that bile acid supplementation in the high-lipid diet can effectively enhance the digestion and utilization abilities, promote the growth, reduce the fat deposition, and improve the muscle quality of juvenile Schizothorax prenanti under those experimental conditions. Comprehensive analysis of various indices, the optimal supplemental level of bile acid in the high-lipid diet of juvenile Schizothorax prenanti is 150 mg/kg.

Key words： juvenile Schizothorax prenanti; bile acid; high-lipid diet; growth performance; digestive enzyme activities; serum biochemical indices

Cite this article

XIANG Xiao , ZENG Benhe , YANG Lixiang , ZHOU Xinghua , CHEN Jian , WANG Wenjuan . Effects of High-Lipid Diet Supplemented with Bile Acid on Growth Performance, Muscle Nutritional Component Contents, Digestive Enzyme Activities and Serum Biochemical Indices of Juvenile Schizothorax prenanti[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5089 -5099 . DOI: 10.3969/j.issn.1006-267x.2019.11.024

References

[1] 陈佳毅,叶元土,张伟涛,等.不同脂肪(能量)蛋白比饲料对梭鲈幼鱼生长的影响[J].水产学报,2010,34(3):474-480.
[2] 王国霞,王敏,孙育平,等.外源脂肪酶对花鲈生长性能、体组成、血清生化指标、消化酶活性及营养物质表观消化率的影响[J].动物营养学报,2017,29(12):4542-4553.
[3] 王爱民,韩光明,封功能,等.饲料脂肪水平对吉富罗非鱼生产性能、营养物质消化及血液生化指标的影响[J].水生生物学报,2011,35(1):80-87.
[4] 麦康森.水产动物营养与饲料学[M].2版.北京:中国农业出版社,2011:41-53.
[5] 柳阳.脂肪和蛋白质水平对工业化养殖大西洋鲑的效应和机制研究[D].硕士学位论文.青岛:中国科学院海洋研究所,2013.
[6] 周锴,吴莉芳,瞿子惠,等.饲料脂肪水平对鱼类生长、抗氧化及脂肪酸组成影响的研究[J].饲料工业,2018(8):26-31.
[7] 向枭,周兴华,陈建,等.饲料脂肪水平对白甲鱼幼鱼形体指数、脂肪沉积和脂肪代谢酶活性的影响[J].水产学报,2013,37(9):1349-1358.
[8] 汪开毓,苗常鸿,黄锦炉,等.投喂高脂饲料后草鱼主要生化指标和乙酰辅酶A羧化酶1 mRNA表达的变化[J].动物营养学报,2012,24(12):2375-2383.
[9] ROMANSKI K W.The role and mechanism of action of bile acids in the digestive system-bile acids in the gut[J].Advances in Clinical and Experimental Medicine,2008,17(1):83-89.
[10] 孙建珍.添加胆汁酸对大菱鲆(Scophthalmus maximus)幼鱼脂肪利用能力的影响[D].硕士学位论文.上海:上海海洋大学,2014.
[11] CHARLTON-MENYS V,DURRINGTON P N.Human cholesterol metabolism and therapeutic molecules[J].Experimental Physiology,2007,93(1):27-42.

[12] BEGLEY M,GAHAN C G M,HILL C.The interaction between bacteria and bile[J].FEMS Microbiology Reviews,2005,29(4):625-651.

[13] POUPON R E,LINDOR K D,PARÉS A,et al.Combined analysis of the effect of treatment with ursodeoxycholic acid on histologic progression in primary biliary cirrhosis[J].Journal of hepatology,2003,39(1):12-16.

[14] 曾本和,向枭,周兴华,等.高脂饲料中胆汁酸水平对齐口裂腹鱼肠道组织结构及脂肪代谢酶活性的影响[J].水产学报,2016,40(9):1340-1348.
[15] 曾本和,廖增艳,向枭,等.胆汁酸对草鱼(Ctenopharyngodon idellus)的生长性能、肌肉营养成分及消化酶活性的影响[J].渔业科学进展,2017,38(2):99-106.
[16] 陈昊杰.胆汁酸对草鱼生长、脂质代谢及肠道微生物区系的影响[D].硕士学位论文.杨凌:西北农林科技大学,2016.
[17] 郑宗林,曾本和,向枭,等.饲料中胆汁酸添加水平对齐口裂腹鱼幼鱼生长性能、形体指标及体成分的影响[J].动物营养学报,2016,28(8):2423-2430.
[18] 中山大学生物系生化微生物学教研室.生化技术导论[M].北京:人民教育出版社,1979:26-29.
[19] WANG J T,LIU Y J,TIAN L X,et al.Effect of dietary lipid level on growth performance,lipid deposition,hepatic lipogenesis in juvenile cobia (Rachycentron canadum)[J].Aquaculture,2005,249(1/2/3/4):439-447.
[20] 池作授,耿旭,郭云学,等.奥尼罗非鱼仔稚鱼饲料中适宜脂肪水平的研究[J].中国饲料,2010(20):32-36.
[21] 向枭,曾本和,周兴华,等.不同脂肪条件下添加牛磺酸对鲤生长性能、体成分、肝胰脏生化指标及抗氧化能力的影响[J].水产学报,2018,42(12):1927-1939.
[22] DU Z Y,LIU Y J,TIAN L X,et al.Effect of dietary lipid level on growth,feed utilization and body composition by juvenile grass carp (Ctenopharyngodon idella)[J].Aquaculture Nutrition,2015,11(2):139-146.
[23] 何杰.饲料添加胆汁酸对异育银鲫生长、氧化损伤修复、内源胆汁酸代谢以及CyHV-2免疫保护作用的影响[D].硕士学位论文.苏州:苏州大学,2018.
[24] 黄炳山,李宝山,张利民,等.胆汁酸对大菱鲆幼鱼生长、脂肪代谢酶及血清生化的影响[J].上海海洋大学学报,2015,24(5):737-744.
[25] DISTRUTTI E,SANTUCCI L,CIPRIANI S,et al.Bile acid activated receptors are targets for regulation of integrity of gastrointestinal mucosa[J].Journal of Gastroenterology,2015,50(7):707-719.

[26] CHOU B S,SHIAU S Y.Optimal dietary lipid level for growth of juvenile hybrid tilapia,Oreochromis niloticus×Oreochromis aureus[J].Aquaculture,1996,143(2):185-195.

[27] ROMANSKI K W.The role and mechanism of action of bile acids within the digestive system-bile acids in the liver and bile[J].Advances in Clinical and Experimental Medicine,2007,16(6):793-799.
[28] 刘敬盛,杨玉芝,王君荣,等.胆汁酸的营养特性及其在家禽生产中应用的研究进展[J].中国畜牧兽医,2010,37(1):13-16.
[29] 胡田恩,王玲,张春晓,等.饲料中添加胆汁酸对牛蛙生长性能、体组成和营养物质表观消化率的影响[J].水生生物学报,2015,39(4):677-685.
[30] BAJOR A,GILLBERG P G,ABRAHAMSSON H.Bile acids:short and long term effects in the intestine[J].Scandinavian Journal of Gastroenterology,2010,45(6):645-664.

[31] 盛怡.高脂饲料引起罗非鱼脂肪蓄积的肠道微生物机制初探[D].硕士学位论文.上海:华东师范大学,2018.
[32] GRISDALE-HELLAND B,SHEARER K D,GATLIN Ⅲ D M G,et al.Effects of dietary protein and lipid levels on growth,protein digestibility,feed utilization and body composition of Atlantic cod (Gadus morhua)[J].Aquaculture,2008,283(1/2/3/4):156-162.
[33] 李艾璇.草鱼对高脂、低脂摄入的代谢适应策略及鳜鱼瘦素的功能初探[D].硕士学位论文.武汉:华中农业大学,2016.
[34] 周书耘,刘永坚,梁海鸥,等.饲料中添加胆汁酸对军曹鱼生长及体组成的影响[J].南方水产,2010,6(4):20-25.
[35] 陈昊杰,田晶晶,周继术,等.高脂日粮添加胆汁酸对草鱼组织脂肪酸组成的影响[J].西北农业学报,2017,26(1):14-24.
[36] 张露露.胆汁酸在大口黑鲈饲料中有效性及耐受性评价[D].硕士学位论文.济南:山东农业大学,2015.
[37] 张辉,单安山,牟振波,等.鱼类脂类代谢调控机制[J].中国饲料,2008(2):41-44.
[38] DEPLANO M,CONNES R,DIAZ J P,et al.Intestinal steatosis in the farm-reared sea bass Dicentrarchus labrax[J].Diseases of Aquatic Organisms,1989,6:121-130.
[39] 曹俊明,林鼎,薛华,等.四种抗脂肪肝物质降低草鱼肝胰脏脂质积累的替代关系[J].水生生物学报,1999,23(2):102-111.
[40] DU Z Y,CLOUET P,ZHENG W H,et al.Biochemical hepatic alterations and body lipid composition in the herbivorous grass carp (Ctenopharyngodon idella) fed high-fat diets[J].British Journal of Nutrition,2006,95(5):905-915.

[41] 郭永丽.外源胆汁酸制剂对草鱼生长、抗氧化能力及脂肪代谢的影响[D].硕士学位论文.广州:华南农业大学,2008.
[42] WATANABE M,HOUTEN S M,WANG L,et al.Bile acids lower triglyceride levels via a pathway involving FXR,SHP,and SREBP-1c[J].The Journal of Clinical Investigation,2004,113(10):1408-1418.

[43] SCHAAP F G,RENSEN P C N,VOSHOL P J,et al.ApoAV reduces plasma triglycerides by inhibiting very low density lipoprotein-triglyceride (VLDL-TG) production and stimulating lipoprotein lipase-mediated VLDL-TG hydrolysis[J].Journal of Biological Chemistry,2004,279(27):27941-27947.

[44] SIRVENT A,CLAUDEL T,MARTIN G,et al.The farnesoid X receptor induces very low density lipoprotein receptor gene expression[J].FEBS Letters,2004,566(1/2/3):173-177.
[45] 张国安,王继芬,王振业,等.红鱼粉型饲料中添加胆汁酸对大菱鲆生长及肝功能的影响[J].水产科学,2018,37(5):599-604.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References