Effects of Short-Term Ice Water Stimulation on Blood Routine, Serum Biochemical and Immune Indexes of Canines

HE Shaojun; WU Mingming; SONG Kai; DING Jinxue

doi:10.3969/j.issn.1006-267x.2019.11.029

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 11: 5142 - 5150

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.029

Effects of Short-Term Ice Water Stimulation on Blood Routine, Serum Biochemical and Immune Indexes of Canines

HE Shaojun ,
WU Mingming ,
SONG Kai ,
DING Jinxue

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College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China

Received date: 2019-04-15

Online published: 2019-11-19

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Abstract

The present study was to explore the effects of short-term ice water stimulation on blood routine, serum biochemical and immune indexes of canines. In this experiment, eight healthy Chinese rural dogs were selected and the blood samples were collected from the jugular vein for the determination of canine blood routine and serum biochemical and immune indexes before ice water stimulation, at the end of ice water stimulation (after 0.5 h in ice water) and 0.5, 1.0, 3.0, and 7.0 h after the ice water stimulation. The results showed as follows:compared with before ice water stimulation, red blood cells count (RBC), hemoglobin concentration (HGB), hematocrit(HCT), lymphocytes count (Lym), lymphocyte percentage (Lym%), monocyte percentage (Mon%) in blood and the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) and the contents of urea, uric acid (UA), creatinine, complement 3 (C3) in serum were significantly increased at the end of ice water stimulation (P<0.05); at each recovery time point (0.5, 1.0, 3.0 and 7.0 h), serum AST activities and urea content were significantly increased (P<0.05); at recovery time of 1.0, 3.0 and 7.0 h, blood HCT, RBC, HGB and Lym% were significantly decreased (P<0.05), while blood white blood cells count (WBC), granulocyte count (Gran) and granulocyte percentage (Gran%) were significantly increased (P<0.05); serum creatine kinase (CK) activity and C3 content were significantly increased at recovery time of 0.5, 1.0 and 3.0 h (P<0.05), however, serum creatinine content and ALP activity were significantly decreased at recovery time of 1.0, 3.0 and 7.0 h (P<0.05). Compared with the end of ice water stimulation, blood RBC and Lym were significantly decreased at each recovery time points (P<0.05), and serum creatinine content and ALP activity were significantly decreased (P<0.05); however, blood HGB, HCT, Lym% and Mon% were decreased significantly at recovery time of 1.0, 3.0 and 7.0 h (P<0.05). The results indicate that short-term ice water stimulation can significantly change some blood routine, serum biochemical and immune indexes of canines, and suggest that the animals should in time to correct the changes of blood routine and serum biochemical and immune indexes, enhance the body immunity, and alleviate the continuous harm when animals are stimulated by ice water.

Key words： canine; short-term ice water stimulation; blood routine indexes; serum biochemical indexes; serum immune indexes

Cite this article

HE Shaojun , WU Mingming , SONG Kai , DING Jinxue . Effects of Short-Term Ice Water Stimulation on Blood Routine, Serum Biochemical and Immune Indexes of Canines[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5142 -5150 . DOI: 10.3969/j.issn.1006-267x.2019.11.029

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