Differentially Expressed Genes in Vitamin D Regulating Calcium and Phosphorus Absorption in Duodenum of Broiler Chickens Using RNA-Sequencing

YANG Xue; WANG Xiaona; ZHANG Ning; QU Hongxia; SHI Chuanxin; ZHANG Jinliang; YAN Yongfeng; ZHENG Yongxiang; WANG Zhixiang; HAN Jincheng

doi:10.3969/j.issn.1006-267x.2019.11.036

2019 , Vol. 31 >Issue 11: 5202 - 5213

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.036

Differentially Expressed Genes in Vitamin D Regulating Calcium and Phosphorus Absorption in Duodenum of Broiler Chickens Using RNA-Sequencing

YANG Xue ,
WANG Xiaona ,
ZHANG Ning ,
QU Hongxia ,
SHI Chuanxin ,
ZHANG Jinliang ,
YAN Yongfeng ,
ZHENG Yongxiang ,
WANG Zhixiang ,
HAN Jincheng

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1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China;
2. Department of Animal Science, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China;
3. Department of Biotechnology and Animal Science, National Ilan University, Yilan 26047, China

Received date: 2019-04-24

Online published: 2019-11-19

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Abstract

This study was conducted to investigate the candidate genes and signaling pathways in vitamin D regulating calcium and phosphorus absorption in small intestine of broiler chickens using RNA-sequencing technology. Ross 308 broiler chickens at 1 to 16 days of age were used as experimental animals. Three levels[0 (control group), 5 and 10 μg/kg] of 1,25-dihydroxycholecalciferol[1,25-(OH)₂-D₃] were added to the basal diet without vitamin D. Broiler chickens were killed and duodenal mucosa was collected at 16 days of age. Gene ontology (GO) function annotation and kyoto encyclopedia of genes and genomes (KEGG) signal pathway enrichment of differentially expressed genes were carried out using RNA-sequencing technology. Results showed as follows:1) compared with the control group, the addition of 5 and 10 μg/kg of 1,25-(OH)₂-D₃ increased significantly growth performance (P<0.05) and improved tibia and femur mineralization. The weight, length and ash content of tibia and femur of broilers in 10 μg/kg of 1,25-(OH)₂-D₃ group were significantly lower than those in 5 μg/kg 1,25-(OH)₂-D₃ group (P<0.05). 2) A total of 1 029, 939, and 642 differentially expressed genes were screened when comparing the three levels of 1,25-(OH)₂-D₃ (0 vs. 5 μg/kg, 0 vs. 10 μg/kg, and 5 vs. 10 μg/kg), respectively. 3) GO analysis identified candidate genes regulating calcium and phosphorus absorption and metabolism, including nuclear vitamin D receptor (VDR), protein disulfide-isomerase A3 (PDIA3), type Ⅱb sodium phosphate transporter protein NaPi-Ⅱb (SLC34A2), inorganic phosphorus transporter carrier protein 2 (SLC20A2), calbindin D28k (CALB1), ATPase plasma membrane Ca²⁺ transporting 1 (ATP2B1), ATPase plasma membrane Ca²⁺ transporting 2 (ATP2B2), solute carrier family 8 member A1 NCX1 (SLC8A1), fibroblast growth factor 1 (FGF1) and fibroblast growth factor 19 (FGF19). 4) KEGG analysis indicated pathways associated with the intestinal calcium and phosphorus absorption and metabolism of broiler chickens, including mitogen-activated protein kinase (MAPK) signaling pathway, cyclic adenosine monophosphate (cAMP) signaling pathway or cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) signaling pathway, and cyclic guanosine monophosphate-protein kinase G (cGMP-PKG) signaling pathway. 5) The results of qRT-PCR were in accordance with those of RNA-seq. In summary, the differentially expressed genes and signaling pathways in vitamin D regulating calcium and phosphorus absorption in small intestine of broiler chickens are screened. These data will be helpful to improve calcium and phosphorus absorption and retention in poultry diets.

Key words： broiler chicken; duodenum; calcium and phosphorus metabolism; differentially expressed gene; signaling pathway

Cite this article

YANG Xue , WANG Xiaona , ZHANG Ning , QU Hongxia , SHI Chuanxin , ZHANG Jinliang , YAN Yongfeng , ZHENG Yongxiang , WANG Zhixiang , HAN Jincheng . Differentially Expressed Genes in Vitamin D Regulating Calcium and Phosphorus Absorption in Duodenum of Broiler Chickens Using RNA-Sequencing[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5202 -5213 . DOI: 10.3969/j.issn.1006-267x.2019.11.036

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