Effects of Serotonin Precursor on mRNA and Protein Expressions of Liver Gluconeogenesis Key Genes of Perinatal Ewes

SANG Dan; NAMIRGA; SUN Haizhou; JIN Lu; ZHANG Chongzhi; ZHANG Chunhua; RU Ting; JIA Chunlei; AO Changjin

doi:10.3969/j.issn.1006-267x.2019.11.037

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 11: 5214 - 5220

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.037

Effects of Serotonin Precursor on mRNA and Protein Expressions of Liver Gluconeogenesis Key Genes of Perinatal Ewes

SANG Dan ,
NAMIRGA ,
SUN Haizhou ,
JIN Lu ,
ZHANG Chongzhi ,
ZHANG Chunhua ,
RU Ting ,
JIA Chunlei ,
AO Changjin

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1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Animal Nutrition and Feed Research Institution of Inner Mongolia Academy of Agriculture and Animal Husbandry Academy, Hohhot 010031, China;
3. Urat Front Banner Livestock Improvement Station, Bayannur 014400, China

Received date: 2019-06-26

Online published: 2019-11-19

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Abstract

This study was conducted to investigate the effects of serotonin (5-HT) precursor on mRNA and protein expressions of liver gluconeogenesis key genes of perinatal ewes. Thirty good body condition Bamei ewes with a body weight of (64.52±4.11) kg in the same perinatal period were randomly divided into 3 groups according to their body weight, which were control group, 5-hydroxy-tryptophan (5-HTP) group (perfused 5-HTP) and tryptophan (Trp) group (perfused Trp), respectively, each group contained 10 ewes. Ewes jugular vein perfusion was conducted from prenatal day 7 to postpartum day 0, the perfusion dose of 5-HTP and Trp was 0.178 mg/kg BW, and the concentration was 0.1 mg/mL; the control group was perfused with the same dose of saline. The experiment lasted form prenatal day 7 to postpartum day 30. The results showed as follows:1) the mRNA expressions of phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G6P) and pyruvate carboxylase (PC) in liver of 5-HTP group and Trp group were significantly higher than those of the control group (P<0.05). 2) The protein expressions of PEPCK, G6P and PC in liver of 5-HTP group and Trp group were significantly higher than those of the control group (P<0.05), the liver PEPCK protein expression of Trp group was significantly lower than that of 5-HTP group (P<0.05), and the liver G6P protein expression of Trp group was significantly higher than that of 5-HTP group (P<0.05). In conclusion, 5-HT precursor plays a role in promoting the liver gluconeogenesis not only at the transcriptional level, but also at the translation level.

Key words： serotonin precursor; liver gluconeogenesis; perinatal period; ewes

Cite this article

SANG Dan , NAMIRGA , SUN Haizhou , JIN Lu , ZHANG Chongzhi , ZHANG Chunhua , RU Ting , JIA Chunlei , AO Changjin . Effects of Serotonin Precursor on mRNA and Protein Expressions of Liver Gluconeogenesis Key Genes of Perinatal Ewes[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5214 -5220 . DOI: 10.3969/j.issn.1006-267x.2019.11.037

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