Effects of Heat Treatment on Expression of Appetite Regulation- and Adenosine Monophosphate Activated Protein Kinase Signaling Pathway-Related Genes in Hypothalamus

ZHANG Hui; LI Xianlei; ZHOU Huajin; WANG Jiguang; HAO Yangyang; SONG Zhigang; SU Pengcheng

doi:10.3969/j.issn.1006-267x.2019.11.038

2019 , Vol. 31 >Issue 11: 5221 - 5228

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.038

Effects of Heat Treatment on Expression of Appetite Regulation- and Adenosine Monophosphate Activated Protein Kinase Signaling Pathway-Related Genes in Hypothalamus

ZHANG Hui ,
LI Xianlei ,
ZHOU Huajin ,
WANG Jiguang ,
HAO Yangyang ,
SONG Zhigang ,
SU Pengcheng

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College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2019-04-27

Online published: 2019-11-19

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Abstract

This experiment was conducted to investigate the effects of heat treatment on the expression of appetite regulation- and adenosine monophosphate activated protein kinase (AMPK) signaling pathway-related genes in hypothalamus. Hypothalamus neuron cells of broilers were cultured in vitro with six porous plates, and the hypothalamus neuron cell culture model was established. Neuronal cells which cultured in vitro were divided into control group and heat treatment group, and cultured in incubator (37℃, 5%CO₂). Each group had six replicates, and each replicate corresponded to a hole. Heat treatment was carried out on the 5th day of culture, the neuronal cells in the control group were cultured at 37℃, and the neuronal cells in the heat treatment group were cultured at 43℃. After 1.5 h of heat treatment, neuronal cells were collected and the mRNA relative expression levels of regulation- and AMPK signaling pathway-related genes in hypothalamus neuronal cells were detected by real-time quantitative PCR (RT-qPCR) technique. The results showed as follows:compared with the control group, 1) heat treatment significantly up-related the mRNA relative expression levels of hypothalamus appetite-stimulating gene agouti-related protein (AgRP) and appetite-suppressing gene proopiomelanocortin (POMC) (P<0.05); 2) heat treatment significantly down-related the mRNA relative expression levels of hypothalamus AMPK signaling pathway-related genes AMPKα2 and fatty acid synthase (FAS) (P<0.05); 3) heat treatment significantly up-related the mRNA relative expression levels of hypothalamus AMPK signaling pathway upstream factor serine/threonine protein kinase 1 (AKT1) (P<0.05). It is concluded that heat treatment can significantly up-regulate the expression of appetite-stimulating gene AgRP and appetite-suppressing gene POMC in hypothalamus, and it also can significantly down-regulate the expression of AMPKα2 and FAS genes and up-regulate the expression of AKT1 gene in AMPK signaling pathway in hypothalamus.

Key words： hypothalamus; appetite regulation-related genes; AMPK signaling pathway-related genes; cell culture in vitro; heat treatment

Cite this article

ZHANG Hui , LI Xianlei , ZHOU Huajin , WANG Jiguang , HAO Yangyang , SONG Zhigang , SU Pengcheng . Effects of Heat Treatment on Expression of Appetite Regulation- and Adenosine Monophosphate Activated Protein Kinase Signaling Pathway-Related Genes in Hypothalamus[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5221 -5228 . DOI: 10.3969/j.issn.1006-267x.2019.11.038

References

[1] GAO Q,HORVATH T L.Cross-talk between estrogen and leptin signaling in the hypothalamus[J].American Journal of Physiology-Endocrinology and Metabolism,2008,294(5):E817-E826.
[2] 徐彦博.芍药苷对离体大鼠下丘脑腹内侧核神经元电生理活动的影响[D].硕士学位论文.西安:第四军医大学,2008.
[3] 唐焰.下丘脑神经元在体活动参与摄食调控[D].博士学位论文.上海:华东师范大学,2016.
[4] ELMQUIST J K,MARCUS J N.Rethinking the central causes of diabetes[J].Nature Medicine,2003,9(6):645-647.

[5] SUZUKI K,JAYASENA C N,BLOOM S R.Obesity and appetite control[J].Experimental Diabetes Research,2012,2012:824305.
[6] DENBOW D M,MEADE S,ROBERTSON A,et al.Leptin-induced decrease in food intake in chickens[J].Physiology & Behavior,2000,69(3):359-362.

[7] GERETS H H J,PEETERS K,ARCKENS L,et al.Sequence and distribution of pro-opiomelanocortin in the pituitary and the brain of the chicken (Gallus gallus)[J].Journal of Comparative Neurology,2000,417(2):250-262.

[8] KRISTENSEN P,JUDGE M E,THIM L,et al.Hypothalamic CART is a new anorectic peptide regulated by leptin[J].Nature,1998,393(6680):72-76.

[9] HARDIE D G,SCOTT J W,PAN D A,et al.Management of cellular energy by the AMP-activated protein kinase system[J].FEBS Letters,2003,546(1):113-120.

[10] DZAMKO N,VAN DENDEREN B J W,HEVENER A L,et al.AMPK β1 deletion reduces appetite,preventing obesity and hepatic insulin resistance[J].Journal of Biological Chemistry,2010,285(1):115-122.

[11] XIAO B,SANDERS M J,UNDERWOOD E,et al.Structure of mammalian AMPK and its regulation by ADP[J].Nature,2011,472(7342):230-233.

[12] MINOKOSHI Y,ALQUIER T,FURUKAWA N,et al.AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus[J].Nature,2004,428(6982):569-574.

[13] AHIMA R S,OSEI S Y.Molecular regulation of eating behavior:new insights and prospects for therapeutic strategies[J].Trends in Molecular Medicine,2001,7(5):205-213.

[14] CEJAS P J,MARTINEZ M,KARMALLY S,et al.Lumbar transplant of neurons genetically modified to secrete brain-derived neurotrophic factor attenuates allodynia and hyperalgesia after sciatic nerve constriction[J].Pain,2000,86(1/2):195-210.
[15] BURDYGA G,DE LARTIGUE G,RAYBOULD H E,et al.Cholecystokinin regulates expression of Y2 receptors in vagal afferent neurons serving the stomach[J].Journal of Neuroscience,2008,28(45):11583-11592.

[16] 魏凤,郭广君,吕素芳,等.猪原代神经细胞的体外培养与鉴定[J].中国兽医学报,2016,36(8):1367-1370.
[17] MAKIMURA H,MIZUNO T M,ROBERTS J,et al.Adrenalectomy reverses obese phenotype and restores hypothalamic melanocortin tone in leptin-deficient ob/ob mice[J].Diabetes,2000,49(11):1917-1923.

[18] TSIGOS C,CHROUSOS G P.Hypothalamic-pituitary-adrenal axis,neuroendocrine factors and stress[J].Journal of Psychosomatic Research,2002,53(4):865-871.

[19] JEONG J H,LEE D K,LIU S M,et al.Activation of temperature-sensitive TRPV1-like receptors in ARC POMC neurons reduces food intake[J].PLoS Biology,2018,16(4):e2004399.
[20] 周华金,孙晓蕾,钟光,等.大麻素受体1拮抗剂利莫那班对肉仔鸡下丘脑食欲调控因子和腺苷酸激活蛋白激酶基因表达的影响[J].动物营养学报,2019,31(1):258-265.
[21] LEVINE A S,MORLEY J E.Stress-induced eating in rats[J].American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,1981,241(1):R72-R76.
[22] KOLA B,HUBINA E,TUCCI S A,et al.Cannabinoids and ghrelin have both central and peripheral metabolic and cardiac effects via AMP-activated protein kinase[J].Journal of Biological Chemistry,2005,280(26):25196-25201.

[23] 余春莲.AMPK信号通路负向调节热应激诱导仔猪睾丸支持细胞乳酸分泌[D].硕士学位论文.重庆:西南大学,2017.
[24] LEE-YOUNG R S,BONNER J S,MAYES W H,et al.AMP-activated protein kinase (AMPK)α2 plays a role in determining the cellular fate of glucose in insulin-resistant mouse skeletal muscle[J].Diabetologia,2013,56(3):608-617.

[25] 王启军.高温环境对不同生长阶段北京油鸡脂肪沉积及脂质代谢的影响[D].硕士学位论文.杨凌:西北农林科技大学,2006.
[26] BAZIZ H A,GERAERT P A,PADILHA J C F,et al.Chronic heat exposure enhances fat deposition and modifies muscle and fat partition in broiler carcasses[J].Poultry Science,1996,75(4):505-513.

[27] LÓPEZ M,LELLIOTT C J,VIDAL-PUIG A.Hypothalamic fatty acid metabolism:a housekeeping pathway that regulates food intake[J].BioEssays,2007,29(3):248-261.

[28] 秦玉辉.蛋鸡体内AMPK酶活分布及应激对AMPK酶活影响研究[D].硕士学位论文.雅安:四川农业大学,2003.
[29] YANG C S,LAM C K L,CHARI M,et al.Hypothalamic AMP-activated protein kinase regulates glucose production[J].Diabetes,2010,59(10):2435-2443.

[30] CHEN L,JIAO Z H,ZHENG L S,et al.Structural insight into the autoinhibition mechanism of AMP-activated protein kinase[J].Nature,2009,459(7250):1146-1149.

[31] YAMAUCHI T,KAMON J,MINOKOSHI Y,et al.Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase[J].Nature Medicine,2002,8(11):1288-1295.

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