Regulation of Mannan Oligosaccharides on Rumen Bacterial Flora Induced by High Concentrate in Low Milk Fat Dairy Cows

  • XU Xiaofeng ,
  • GUO Tingting ,
  • GUO Cheng ,
  • JI Wulong ,
  • ZHANG Lili
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  • College of Agriculture, Ningxia University, Yinchuan 750021, China

Received date: 2019-05-08

  Online published: 2019-11-19

Abstract

This experiment was aimed to reveal the regulation of mannan oligosaccharides (MOS) on the rumen microbial flora structure of concentrate-induced low-fat dairy cows. Four Chinese Holstein dairy cows with similar lactation stages and the same parity were selected. A self-controlled trials were used, dairy cows fed a basal diet (control group), used starch to induce low milk fat (induction group), and then added MOS to regulate rumen fermentation and milk fat (regulatory group). The results showed that MOS affected the rumen microbial flora structure of concentrate-induced low-fat dairy cows. At phylum level, compared with induction group, the abundance of Bacteroidetes (P<0.05), Spirochaetae (P>0.05) and Actinobacteria (P>0.05)were reduced in the rumen of low-fat dairy cows; the abundance of Firmicutes was significantly increased (P<0.05), and significantly higher than that in control group (P<0.05); the abundance of Fibrobacteres was significantly increased compared with the induction group and control group (P<0.01). At genus level, compared with induction group, the abundance of Prevotella_1, Prevotella_7, Lactobacillus and Lactococcus in regulatory group were significantly reduced (P<0.01); the abundances of Prevotella_UCG-001 and Prevotella_UCG-003 were increased, and the abundance of Prevotella_UCG-001 was significantly higher than that in the control group (P<0.01), the abundance of Prevotella_UCG-003 was significantly higher than that in the control group and induction group (P<0.01); the abundance of Succinclasticum was significantly increased (P<0.05). Functional prediction of the rumen flora revealed that low-fat dairy cows supplemented with MOS reduced the abundance of the flora involved in starch and sucrose metabolism. It can be seen that the addition of MOS can regulate the structure of the bacterial flora in the rumen, alleviate the decrease in the abundance of the fiber-degrading bacteria caused by feeding a large amount of starch, and has a certain effect on improving the degradation rate of cellulose in the rumen. Reducing the abundance of acid-producing bacteria has a positive effect on stabilizing the rumen pH.

Cite this article

XU Xiaofeng , GUO Tingting , GUO Cheng , JI Wulong , ZHANG Lili . Regulation of Mannan Oligosaccharides on Rumen Bacterial Flora Induced by High Concentrate in Low Milk Fat Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5245 -5255 . DOI: 10.3969/j.issn.1006-267x.2019.11.041

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