Probiotic Evaluation of Bacillus firmus CX10

  • MA Yujun ,
  • LIANG Zijing ,
  • ZHANG Kai ,
  • LI Jie ,
  • MA Xiaojun ,
  • WANG Lei ,
  • ZHANG Kang ,
  • ZHANG Jingyan ,
  • LI Jianxi
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  • 1. Lanzhou Institute of Husbandry and Pharmaceutical Science, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;
    2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2019-04-03

  Online published: 2019-11-19

Abstract

The aim of this study was to evaluate the probiotics of Bacillus firmus CX10 (BF-CX10) by oral gavage in mice, measuring their body weight, viscera index, serum enzyme activity and immune cytokine expression, as well as the structure and diversity of fecal flora. A total of 50 female BALB/c mice of 18 to 20 g were randomly divided into control group, Escherichia coli self-healing (ESH) group, low-dose BF-CX10 (LBF) group, medium-dose BF-CX10 (MBF) group and high-dose BF-CX10 (HBF) group, with 10 mice in each group. Mice in the control group were given normal saline throughout the whole period, those in the ESH group were given Escherichia coli O78 on the first 3 days and normal saline on the second 3 days, and those in the LBF group, MBF group and HBF group were given 1.5×104, 1.5×106 and 1.5×108 CFU/mL BF-CX10 on the first 3 days respectively and Escherichia coli O78 on the second 3 days. The gavage dose was 0.02 mL/g BW. Mice were killed at day 7. The results show that compared with the ESH group, mice in BF-CX10 groups got the stable weight gain, and the indices of kidney and heart as well as serum activity of acid phosphatase (ACP), alkaline phosphatase (AKP) and glutathione peroxidase (GSH-Px) of mice in BF-CX10 groups were increased significantly (P<0.05), while serum lysozyme (LYZ) activity of mice in BF-CX10 groups was no significant difference (P>0.05). Compared with the ESH group, BF-CX10 gavage significantly up-regulated the mRNA relative expression levels of interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4) and interleukin-10 (IL-10) (P<0.05), and significantly down-regulated the interleukin-12 (IL-12) mRNA relative expression level (P<0.05) in mouse kidney. The diversity of mouse fecal flora sequencing showed that the measured species number had a significant difference between ESH group and HBF group (P<0.05). Species annotation results showed that at the phylum level, Bacteroidetes and Firmicutes were the dominant bacteria in LBF group and HBF group, among which the Firmicutes were mainly Bacillaceae and Lactobacillaceae. The results of bacterial function prediction showed that the functional clustering of HBF group was dominated by metabolism. The results suggest that BF-CX10 gavage can maintain normal body weight gain, increase serum enzyme activity and immune function of mice, and have a significant probiotic effect on the recovery of intestinal microbial population in mice.

Cite this article

MA Yujun , LIANG Zijing , ZHANG Kai , LI Jie , MA Xiaojun , WANG Lei , ZHANG Kang , ZHANG Jingyan , LI Jianxi . Probiotic Evaluation of Bacillus firmus CX10[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5310 -5320 . DOI: 10.3969/j.issn.1006-267x.2019.11.048

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