Effects of Saccharomyces cerevisiae Supplementation in Diets with Different Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratios on in Vitro Ruminal Fermentation of Sheep

ZHENG Weicai; HAO Xiaoyan; ZHANG Chunxiang; XIANG Binwei; ZHANG Wenjia; WEN Haoyu; ZHANG Jianxin

doi:10.3969/j.issn.1006-267x.2019.11.053

2019 , Vol. 31 >Issue 11: 5354 - 5366

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.053

Effects of Saccharomyces cerevisiae Supplementation in Diets with Different Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratios on in Vitro Ruminal Fermentation of Sheep

ZHENG Weicai ,
HAO Xiaoyan ,
ZHANG Chunxiang ,
XIANG Binwei ,
ZHANG Wenjia ,
WEN Haoyu ,
ZHANG Jianxin

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1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. Animal Husbandry Bureau of Youyu County, Youyu 037200, China;
3. College of Animal Science and Veterinary Medicine, Northwest A&F University, Yangling 712100, China

Received date: 2019-04-15

Online published: 2019-11-19

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Abstract

The objective of present study was to investigate the effects of Saccharomyces cerevisiae (S. cerevisiae) supplementation in diets with different non-fiber carbohydrate/neutral detergent fiber ratios (NFC/NDF) on in vitro ruminal fermentation of sheep. The method of double-factor design was adopted in this experiment. Fifteen fermentation substrates were prepared by adding five levels[0 (B1), 2×10¹¹ (B2), 4×10¹¹ (B3), 6×10¹¹ (B4) and 8×10¹¹ CFU/kg (B5)] of S. cerevisiae to three diets with different NFC/NDF[0.79 (A1), 0.89 (A2) and 1.10 (A3)]. Gas production parameters, methane (CH₄) production, ruminal fermentation indices and nutrient degradation rates were determined at 48 h fermentation in vitro. The result showed as follows:1) dietary NFC/NDF and S. cerevisiae supplemental level had significant effects on cumulative gas production at 24 and 48 h and gas production parameters (P<0.05), and the interaction between them had significant effects on cumulative gas production at 24 and 48 h (P<0.05), but had no significant effects on gas production parameters (P>0.05). 2) CH₄ production was not significantly affected by dietary NFC/NDF, S. cerevisiae supplemental level and their interaction (P>0.05). The concentrations of total volatile fatty acid (TVFA) and propionic acid in in vitro fermentation fluid were gradually increased with the increase of dietary NFC/NDF, while the concentrations of acetic acid, butyric acid and the ratio of acetic acid to propionic acid were gradually decreased. When the S. cerevisiae supplemental level was 6×10¹¹ CFU/kg, the concentrations of TVFA and propionic acid in in vitro fermentation fluid were the highest, the acetic acid concentration and the ratio of acetic acid to propionic acid were the lowest. The acetic acid concentration in B4 group was significantly higher than that in B1, B2 and B5 groups (P<0.05), and the ratio of acetic acid to propionic acid in B4 group was significantly higher than that in B1, B3 and B5 groups (P<0.05). The interaction between dietary NFC/NDF and S. cerevisiae supplemental level had no significant effects on CH₄ production, the concentrations of TVFA, acetic acid, propionic acid, butyric acid and the ratio of acetic acid to propionic acid in in vitro fermentation fluid (P>0.05). 3) With the increase of dietary NFC/NDF, the pH and ammonia nitrogen (NH₃-N) concentrations in in vitro fermentation fluid were gradually decreased, and those in A1 group were significantly higher than those in A2 and A3 groups (P<0.05). The microprotein (MCP) concentration in in vitro fermentation fluid was the largest when dietary NFC/NDF was 0.89, and significantly higher than that when dietary NFC/NDF were 0.79 and 1.10. S. cerevisiae supplemental level had significant effects on the concentrations of NH₃-N and MCP in in vitro fermentation fluid (P<0.05). The concentration of NH₃-N was the lowest and the concentration of MCP was the highest when S. cerevisiae supplemental level was 6×10¹¹ CFU/kg. The interaction between dietary NFC/NDF and S. cerevisiae supplemental level had no significant effect on pH (P>0.05), but had significant effects on the concentrations of NH₃-N and MCP in in vitro fermentation fluid (P<0.05). 4) Dietary NFC/NDF had significant effects on the dry matter degradation rate (DMD), neutral detergent fiber degradation rate (NDFD) and acid detergent fiber degradation rate (ADFD) of in vitro fermentation substrate (P<0.05), S. cerevisiae supplemental level had significant effects on indicators except for NDFD (P<0.05), and the interaction between them had significant effect on DMD (P<0.05). In conclusion, dietary NFC/NDF, S. cerevisiae supplemental level and their interaction will affect in vitro ruminal fermentation. The optimal supplementation of S. cerevisiae can promote in vitro ruminal fermentation, stabilize fermentation fluid pH and improve feed utilization. On the whole, when dietary NFC/NDF is 0.89 or 1.10 and S. cerevisiae supplemental level is 6×10¹¹ CFU/kg, in vitro ruminal fermentation of sheep has the best effect.

Key words： NFC/NDF; Saccharomyces cerevisiae; sheep; in vitro ruminal fermentation

Cite this article

ZHENG Weicai , HAO Xiaoyan , ZHANG Chunxiang , XIANG Binwei , ZHANG Wenjia , WEN Haoyu , ZHANG Jianxin . Effects of Saccharomyces cerevisiae Supplementation in Diets with Different Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratios on in Vitro Ruminal Fermentation of Sheep[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5354 -5366 . DOI: 10.3969/j.issn.1006-267x.2019.11.053

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