Analysis on Structure and Composition of Rumen Bacterial Flora before and after Acute Rumen Acidosis in Goats

LI Xiaoyu; SU Siyi; ZHONG Fuchao; HUANG Yingying; LIU Leihong; LI Wenwen; LIN Jiahui; LI Jinchun; LIU Ya; FENG Shibin; WANG Xichun; WU Jinjie; SUN Pei; LI Yu

doi:10.3969/j.issn.1006-267x.2019.11.054

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 11: 5367 - 5377

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.054

Analysis on Structure and Composition of Rumen Bacterial Flora before and after Acute Rumen Acidosis in Goats

LI Xiaoyu ,
SU Siyi ,
ZHONG Fuchao ,
HUANG Yingying ,
LIU Leihong ,
LI Wenwen ,
LIN Jiahui ,
LI Jinchun ,
LIU Ya ,
FENG Shibin ,
WANG Xichun ,
WU Jinjie ,
SUN Pei ,
LI Yu

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College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2019-05-10

Online published: 2019-11-19

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Abstract

Rumen microecological balance is an important guarantee for goat health. This study aimed to analyze the changes of rumen bacterial flora before and after acute rumen acidosis in goats by using 16S rRNA sequencing technology. Four healthy Boer goats were selected, and the self-designed before-after control experiment design was adopted. Four portions of tumor-producing fluid collected before modeling were set as healthy group, and four portions of tumor-producing fluid collected after modeling were set as rumen acidosis group. The goat acute rumen acidosis model was established by infusing corn flour, and the amount of corn flour was determined by the ratio of 40 g/kg BW. The method of rumen intubation was used for the collection of gastric juice and corn flour irrigation. The results showed that the diversity and similarity of rumen bacteria flora decreased after acute rumen acidosis. Streptococcus and Lactobacillales under Firmicutes became the dominant bacteria flora, Succinivibrionaceae and Mitochondria under Proteobacteria grew moderately, Prevotella under Bacteroidetes showed no significant difference in relative abundance after acute rumen acidosis (P>0.05). LEfSe analysis showed that Fibrobacteraceae under Fibrobacteres became one of the influencing factors of the difference of rumen bacterial flora composition before and after acute rumen acidosis in goats. PICRUSt analysis found that the rumen bacteria flora may maintain normal survival and reproduction by increasing the utilization of carbohydrates and amino acids when the functions of protein synthesis, processing and degradation were inhibited. It can be seen that the composition, structure and function of rumen bacteria flora have obvious changes compared with the healthy state after the induced acute rumen acidosis in goats.

Key words： goat; acute rumen acidosis; rumen bacterial flora; 16S rRNA

Cite this article

LI Xiaoyu , SU Siyi , ZHONG Fuchao , HUANG Yingying , LIU Leihong , LI Wenwen , LIN Jiahui , LI Jinchun , LIU Ya , FENG Shibin , WANG Xichun , WU Jinjie , SUN Pei , LI Yu . Analysis on Structure and Composition of Rumen Bacterial Flora before and after Acute Rumen Acidosis in Goats[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5367 -5377 . DOI: 10.3969/j.issn.1006-267x.2019.11.054

References

[1] 张梦玲.荷斯坦奶牛胃肠道细菌菌群组成与功能的研究[D].硕士学位论文.南京:南京农业大学,2016.
[2] NASROLLAHI S M,ZALI A,GHORBANI G R,et al.Short communication:blood metabolites,body reserves,and feed efficiency of high-producing dairy cows that varied in ruminal pH when fed a high-concentrate diet[J].Journal of Dairy Science,2019,102(1):672-677.

[3] GRILLI D J,FLIEGEROVÁ K,KOPECNÝ J,et al.Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet[J].Anaerobe,2016,42:17-26.
[4] ISHAQ S L,ALZAHAL O,WALKER N,et al.An investigation into rumen fungal and protozoal diversity in three rumen fractions,during high-fiber or grain-induced sub-acute ruminal acidosis conditions,with or without active dry yeast supplementation[J].Frontiers in Microbiology,2017,8:1943.
[5] 闫佰鹏,兰贵生,李发弟,等.反刍动物亚急性瘤胃酸中毒变异性的机制解析[J].动物营养学报,2018,30(5):1655-1661.
[6] BOERMAN J P,POTTS S B,VANDEHAAR M J,et al.Milk production responses to a change in dietary starch concentration vary by production level in dairy cattle[J].Journal of Dairy Science,2015,98(7):4698-4706.

[7] HATEW B,PODESTA S C,VAN LAAR H,et al.Effects of dietary starch content and rate of fermentation on methane production in lactating dairy cows[J].Journal of Dairy Science,2015,98(1):486-499.

[8] FADROSH D W,MA B,GAJER P,et al.An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform[J].Microbiome,2014,2(1):6.
[9] MAGOC T,SALZBERG S L.FLASH:fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2957-2963.

[10] EDGAR R C.UPARSE:highly accurate OTU sequences from microbial amplicon reads[J].Nature Methods,2013,10(10):996-998.

[11] HUO W J,ZHU W Y,MAO S Y.Impact of subacute ruminal acidosis on the diversity of liquid and solid-associated bacteria in the rumen of goats[J].World Journal of Microbiology and Biotechnology,2014,30(2):669-680.

[12] LANGILLE M G I,ZANEVELD J,CAPORASO J G,et al.Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences[J].Nature Biotechnology,2013,31(9):814-821.

[13] 金磊,周美丽,王禄禄,等.不同能量代谢率的山羊瘤胃微生物结构与组成的差异性[J].微生物学通报,2018,45(1):91-101.
[14] 张瑞阳.组学技术研究亚急性瘤胃酸中毒对奶牛瘤胃微生物、代谢和上皮功能的影响[D].博士学位论文.南京:南京农业大学,2015.
[15] BHATT V D,DANDE S S,PATIL N V,et al.Molecular analysis of the bacterial microbiome in the forestomach fluid from the dromedary camel (Camelus dromedarius)[J].Molecular Biology Reports,2013,40(4):3363-3371.

[16] RUSSELL J B,DIEZ-GONZALEZ F.The effects of fermentation acids on bacterial growth[J].Advances in Microbial Physiology,1998,39:205-234.
[17] KIM M,MORRISON M,YU Z T.STATUS of the phylogenetic diversity census of ruminal microbiomes[J].FEMS Microbiology Ecology,2011,76(1):49-63.

[18] EVANS N J,BROWN J M,MURRAY R D,et al.Characterization of novel bovine gastrointestinal tract Treponema isolates and comparison with bovine digital dermatitis treponemes[J].Applied and Environmental Microbiology,2011,77(1):138-147.

[19] NAGARAJA T G,TITGEMEYER E C.Ruminal acidosis in beef cattle:the current microbiological and nutritional outlook[J].Journal of Dairy Science,2007,90(Suppl.1):E17-E38.
[20] TAJIMA K,AMINOV R I,NAGAMINE T,et al.Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR[J].Applied and Environmental Microbiology,2001,67(6):2766-2774.

[21] SADET S,MARTIN C,MEUNIER B,et al.PCR-DGGE analysis reveals a distinct diversity in the bacterial population attached to the rumen epithelium[J].Animal,2007,1(7):939-944.

[22] BULYGINA E S,GALCHENKO V F,GOVORUKHINA N I,et al.Taxonomic studies on methylotrophic bacteria by 5S ribosomal RNA sequencing[J].Journal of General Microbiology,1990,136(3):441-446.

[23] CHIQUETTE J,ALLISON M J,RASMUSSEN M A.Prevotella bryantii 25A used as a probiotic in early-lactation dairy cows:effect on ruminal fermentation characteristics,milk production,and milk composition[J].Journal of Dairy Science,2008,91(9):3536-3543.

[24] BEKELE A Z,KOIKE S,KOBAYASHI Y.Genetic diversity and diet specificity of ruminal Prevotella revealed by 16S rRNA gene-based analysis[J].FEMS Microbiology Letters,2010,305(1):49-57.

[25] 李烨青,奚雨萌,曾涵芳,等.基于16S rRNA测序分析奶牛体外发酵中瘤胃细菌多样性的变化[J].动物营养学报,2018,30(10):4059-4070.
[26] LI Z P,LIU H L,LI G Y,et al.Molecular diversity of rumen bacterial communities from tannin-rich and fiber-rich forage fed domestic Sika deer (Cervus nippon) in China[J].BMC Microbiology,2013,13:151.
[27] ERICKSON A R,CANTAREL B L,LAMENDELLA R,et al.Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease[J].PLoS One,2012,7(11):e49138.
[28] LAMENDELLA R,DOMINGO J W S,GHOSH S,et al.Comparative fecal metagenomics unveils unique functional capacity of the swine gut[J].BMC Microbiology,2011,11:103.
[29] LENG R A,NOLAN J V.Nitrogen metabolism in the rumen[J].Journal of Dairy Science,1984,67(5):1072-1089.

[30] BACH A,CALSAMIGLIA S,STERN M D.Nitrogen metabolism in the rumen[J].Journal of Dairy Science,2005,88(Suppl.1):E9-E21.

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