Effects of Chronic Lead Exposure on Expressions of Thioredoxin and Heat Shock Protein System Genes and Intestinal Microbiota in Macrobrachium nipponense

ZHENG Chunchun; LI Xiaoyu; NIE Huan; ZHOU Houjie; DING Zhili; TIAN Yuying; DU Mingchuan; LIU Zhu

doi:10.3969/j.issn.1006-267x.2019.11.055

2019 , Vol. 31 >Issue 11: 5378 - 5390

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.11.055

Effects of Chronic Lead Exposure on Expressions of Thioredoxin and Heat Shock Protein System Genes and Intestinal Microbiota in Macrobrachium nipponense

ZHENG Chunchun ,
LI Xiaoyu ,
NIE Huan ,
ZHOU Houjie ,
DING Zhili ,
TIAN Yuying ,
DU Mingchuan ,
LIU Zhu

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Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, College of Life Science, Huzhou University, Huzhou 313000, China

Received date: 2019-04-18

Online published: 2019-11-19

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Abstract

The aim of this study was to investigate the effects of chronic lead exposure on expressions of thioredoxin and heat shock protein system genes and intestine microbiota in Macrobrachium nipponense. Juvenile M.nipponense with an average weight of (0.10±0.02) g were randomly divided into three groups in three replicates (50 tails/replicate) for chronic lead exposure trial. The concentration of lead in water were 0 (control group), 13.13 and 26.26 μg/L and exposure time lasted for 60 days. After the exposure trial, quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze the mRNA expressions of thioredoxin (Trx), thioredoxin reductase (TrxR), heat shock protein 60 (HSP60), heat shock cognate protein 70 (HSC70) and heat shock protein 90 (HSP90). Meanwhile, the composition and diversity of intestine microbiota were analyzed by 16s RNA high throughput sequencing. The results showed as follows 1) chronic lead exposure inhibited the mRNA expressions of TrxR and Trx. The mRNA relative expressions of TrxR and Trx in the 26.26 μg/L group were significantly lower than those in the control group (P<0.05). 2) With the increase of lead concentration, the mRNA relative expressions of HSP60 and HSC70 decreased gradually, and the mRNA relative expressions of HSP60 and HSC70 in 26.26 μg/L group were significantly lower than those in control group (P<0.05). But the mRNA relative expressions of HSP90 increased first and then decreased, and the HSP90 mRNA relative expressions in 13.13 μg/L lead group was significantly higher than that in the other two groups (P<0.05). 3) Analysis of intestinal bacteria of all processing groups of M.nipponense at phyla level, three dominant phyla, Proteobacteria, Firmicutes and Tenericutes were observed in the three groups at the phylum level in M.nipponense. It was found that the relative abundance of Aeromonas_unclassified significantly decreased after lead stress by the conventional variance analysis approach. Intestinal metagenome was analyzed by LEfSe approach, and it was found that there were differences in intestinal bacteria between the control group and the 26.26 μg/L group. The results showed that the relative abundant of Enterobacteriales, Enterobacteriaceae, Enterobacter, Citrobacter, Verrucomicrobiae, Verrucomicrobiales, Verrucomicrobiaceae were higher in the control group, and the relative abundant of Deltaproteobacteria, Flavobacteriia, Flavobacteriales, Flavobacteriaceae, Flavobacterium, Bacteroidetes, Phreatobacter were higher in the 26.26 μg/L group. Thus it can be concluded that chronic lead exposure decreases the transcription level of Trx and TrxR genes and regulates the mRNA relative expressions of HSP60, HSC70 and HSP90 in the hepatopancreas of M.nipponense. Regardless of the concentration of the lead exposure, there are core microorganisms in the intestinal tract of M.nipponense, but high-concentration lead exposure will produce bacteria with significant difference in abundance, and the abundance of bacteria to maintain normal metabolism will be reduced, and the bacteria which is related to the degradation of pollutants and the regulation of immune and antioxidant stress will increase.

Key words： heat shock protein; thioredoxin; intestinal microbiota; lead; Macrobrachium nipponense

Cite this article

ZHENG Chunchun , LI Xiaoyu , NIE Huan , ZHOU Houjie , DING Zhili , TIAN Yuying , DU Mingchuan , LIU Zhu . Effects of Chronic Lead Exposure on Expressions of Thioredoxin and Heat Shock Protein System Genes and Intestinal Microbiota in Macrobrachium nipponense[J]. Chinese Journal of Animal Nutrition, 2019 , 31(11) : 5378 -5390 . DOI: 10.3969/j.issn.1006-267x.2019.11.055

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