Effects of Dietary Isoleucine on Ileal Barrier Function of Weaned Piglets Challenged by Rotavirus

MAO Xiangbing; GU Changsong; CHEN Daiwen; YU Bing; HE Jun; ZHENG Ping; YU Jie; LUO Junqiu; LUO Yuheng

doi:10.3969/j.issn.1006-267x.2019.12.014

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 12: 5493 - 5499

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.014

Effects of Dietary Isoleucine on Ileal Barrier Function of Weaned Piglets Challenged by Rotavirus

MAO Xiangbing ,
GU Changsong ,
CHEN Daiwen ,
YU Bing ,
HE Jun ,
ZHENG Ping ,
YU Jie ,
LUO Junqiu ,
LUO Yuheng

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Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Key Laboratory of Animal Disease-Resistant Nutrition and Feed of Ministry of Agriculture and Rural Affairs, Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2019-06-14

Online published: 2019-12-13

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Abstract

This experiment was conducted to determine whether dietary isoleucine alleviated the effects of rotavirus (RV) infection on ileal barrier function of weaned piglets. In cell culture experiment, IPEC-J2 cells were treated by 8 mmol/L L-isoleucine for 24 h. In animal breeding experiment, twenty-eight healthy crossbred (Duroc×Landrace×Yorkshire) piglets weaned at 21 days of age were used in a 2×2 factorial design. The trial factors included diet treatment (adding 1% L-isoleucine diet or adding 0.68% L-alanine diet) and RV challenge (infusing RV or sterile culture solution). On day 15, RV challenge was carried out. The trial duration was 18 days. The results showed that L-isoleucine treatment increased mRNA relative expression of mucin 2, zonula occludens-1 (ZO-1) and occludin in IPEC-J2 cells (P<0.05). RV challenge increased average diarrhea index and serum diamine oxidase activity (P<0.05), enhanced RV nonstructural protein 4 positive rate in ileal mucosa, and reduced mRNA relative expression of mucin 2, ZO-1 and occludin in ileal mucosa of weaned piglets (P<0.05). Dietary isoleucine relieved the effects of RV challenge on average diarrhea index, serum diamine oxidase activity, and mRNA relative expression of mucin 2, ZO-1 and occludin in ileal mucosa (P<0.05), and decreased RV nonstructural protein 4 positive rate in ileal mucosa of weaned piglets. These results suggest that dietary 1% isoleucine can prevent the RV-induced diarrhea, which is associated with the improvement of ileal barrier function of weaned pigs.

Key words： isoleucine; IPEC-J2 cells; weaned piglets; rotavirus; ileal barrier function

Cite this article

MAO Xiangbing , GU Changsong , CHEN Daiwen , YU Bing , HE Jun , ZHENG Ping , YU Jie , LUO Junqiu , LUO Yuheng . Effects of Dietary Isoleucine on Ileal Barrier Function of Weaned Piglets Challenged by Rotavirus[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5493 -5499 . DOI: 10.3969/j.issn.1006-267x.2019.12.014

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