Effects of Compound Nutrients on Growth Performance and Intestinal Health of Diarrhea Piglets

ZHONG Qin; CHEN Daiwen; YU Bing; HE Jun; HUANG Zhiqing; MAO Xiangbing; YU Jie; LUO Yuheng; LUO Junqiu; ZHENG Pin

doi:10.3969/j.issn.1006-267x.2019.12.016

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 12: 5509 - 5518

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.016

Effects of Compound Nutrients on Growth Performance and Intestinal Health of Diarrhea Piglets

ZHONG Qin ,
CHEN Daiwen ,
YU Bing ,
HE Jun ,
HUANG Zhiqing ,
MAO Xiangbing ,
YU Jie ,
LUO Yuheng ,
LUO Junqiu ,
ZHENG Pin

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Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China

Received date: 2019-05-12

Online published: 2019-12-13

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Abstract

The objective of this study was to evaluate the effects of complex nutrients mainly composed of lactic acid, glutamine, enzyme preparation and Bacillus subtilis on the growth performance and intestinal health of diarrhea piglets caused by metamorphic fishmeal. Twenty-two diarrhea piglets were selected from 150 weaned pigs fed with 4% acidified metamorphic fishmeal (acid price=8.67 mg/g and volatile base nitrogen content=1 446.7 mg/kg) for 15 days. Diarrhea piglets with an average body weight of (7.23±0.89) kg were randomly divided into three groups. Pigs in the diarrhea control group (DC group, n=6) were directly slaughtered and sampled, those in the control group (CT group, n=8) were fed a basal diet and the complex nutrient group (NUT group, n=8) were fed the basal diet supplemented with 3% compound nutrients, respectively, for 24 days. The results showed as follows:1) compared with the CT group, the compound nutrients significantly increased the average daily feed intake (ADFI) and average daily gain (ADG) of piglets form days 15 to 24 of the experiment (P<0.05), and tended to decrease the ratio of feed to gain (F/G) during this period (P=0.08); the compound nutrients extremely significantly reduced the diarrhea rate and diarrhea index of piglets from days 15 to 24 of the experiment (P<0.01), and significantly reduced the diarrhea rate and diarrhea index of piglets in the whole period of the trial (P<0.05). 2) Compared with the DC group, the serum urea nitrogen content and diamine oxidase activity of piglets were significantly decreased after feeding normal diet (P<0.05), and the compound nutrients also significantly reduced the serum contents of creatinine, malondialdehyde and D-lactic acid of piglets (P<0.05). The compound nutrients significantly decreased the serum D-lactic acid content of piglets compared with the CT group (P<0.05). 3) Compared with the DC group, the sucrase activity in jejunal mucosa of piglets was significantly increased (P<0.05), but the maltase activity in jejunal mucosa of piglets was significantly decreased after feeding the normal diet (P<0.05), meanwhile, the villus height and crypt depth were extremely significantly increased (P<0.01). Compared with the CT group, the compound nutrients had a significant effect on the recovery of the jejunal goblet cell number (P<0.05). In summary, feeding metamorphic fishmeal cause diarrhea in piglets, decrease intestinal digestive enzyme activity and damage intestinal morphology. Dietary complex nutrients can improve piglet health and growth performance by promoting intestinal morphology recovery and reducing piglet diarrhea.

Key words： compound nutrients; diarrhea; piglets; growth performance; intestinal health

Cite this article

ZHONG Qin , CHEN Daiwen , YU Bing , HE Jun , HUANG Zhiqing , MAO Xiangbing , YU Jie , LUO Yuheng , LUO Junqiu , ZHENG Pin . Effects of Compound Nutrients on Growth Performance and Intestinal Health of Diarrhea Piglets[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5509 -5518 . DOI: 10.3969/j.issn.1006-267x.2019.12.016

References

[1] 刘国庆,马秋刚,简永军,等.不同等级鱼粉对断奶仔猪生长的影响及其持续效应[J].饲料研究,2015(22):33-37.
[2] RAVINDRAN V,KORNEGAY E T.Acidification of weaner pig diets:a review[J].Journal of the Science of Food & Agriculture,2010,62(4):313-322.
[3] DANIELSON A D,PEO E R,Jr.,SHAHANI K M,et al.Anticholesteremic property of Lactobacillus acidophilus yogurt fed to mature boars[J].Journal of Animal Science,1989,67(4):966-974.

[4] IRAPORDA C,ROMANIN D E,RUMBO M,et al.The role of lactate on the immunomodulatory properties of the nonbacterial fraction of kefir[J].Food Research International,2014,62:247-253.
[5] IRAPORDA C,ERREA A,ROMANIN D E,et al.Lactate and short chain fatty acids produced by microbial fermentation downregulate proinflammatory responses in intestinal epithelial cells and myeloid cells[J].Immunobiology,2015,220(10):1161-1169.

[6] 敖翔,李元凤,范明东,等.谷氨酰胺在断奶仔猪上的应用及其机理的研究[J].养猪,2017(3):33-38.
[7] YI J Q,PIAO X S,LI Z C,et al.The effects of enzyme complex on performance,intestinal health and nutrient digestibility of weaned pigs[J].Asian-Australasian Journal of Animal Sciences,2013,26(8):1181-1188.

[8] SHANAHAN F.Probiotics in perspective[J].Gastroenterology,2010,139(6):1808-1812.

[9] 黄丽.腹泻仔猪生长性能和血液指标特征及灌服粪肠球菌对其影响[D].硕士学位论文.南京:南京农业大学,2015.
[10] 谢全喜,亓秀晔,陈振,等.复合微生态制剂对断奶仔猪生长性能、腹泻率、免疫性能和肠道菌群的影响[J].动物营养学报,2017,29(3):850-858.
[11] YUAN L J,KANG S Y,WARD L A,et al.Antibody-secreting cell responses and protective immunity assessed in gnotobiotic pigs inoculated orally or intramuscularly with inactivated human rotavirus[J].Journal of Virology,1998,72(1):330-338.
[12] 廖波,张克英,丁雪梅,等.饲粮添加25-羟基维生素D₃对轮状病毒攻毒和未攻毒断奶仔猪血清和肠内容物抗体和细胞因子水平的影响[J].动物营养学报,2011,23(1):34-42.
[13] 王纯刚.鱼粉与丁酸钠对断奶仔猪生长、肠道发育和胰高血糖素样肽-2的影响[D].硕士学位论文.雅安:四川农业大学,2009.
[14] 舒邓群,游金民,曾球生.断奶仔猪腹泻对其增重的影响[J].江西畜牧兽医杂志,2004(6):6-7.
[15] 黄美州,刘永明,王慧,等.用主成分分析法研究腹泻仔猪血清生化指标[J].中国兽医学报,2015,35(11):1748-1751,1758.
[16] POEZE M,FROON A H M,GREVE J W M,et al.D-lactate as an early marker of intestinal ischaemia after ruptured abdominal aortic aneurysm repair[J].British Journal of Surgery,1998,85(9):1221-1224.

[17] PANDEY K B,RIZVI S I.Markers of oxidative stress in erythrocytes and plasma during aging in humans[J].Oxidative Medicine and Cellular Longevity,2010,3(1):2-12.

[18] 杨光波,陈代文,余冰.叶酸水平对断奶仔猪生长性能及血清组织中蛋白质代谢的指标影响[J].中国畜牧杂志,2011,47(5):24-28.
[19] 薛洋.腹泻仔猪脏器指数、肠道形态和胃肠道内容物pH特征及灌服粪肠球菌对其影响[D].硕士学位论文.南京:南京农业大学,2015.
[20] 计成,周庆,田河山,等.断奶前后乳猪消化道(胰、小肠内容物)几种消化酶活性变化的研究[J].动物营养学报,1987,9(3):712.
[21] HANCZAKOWSKA E,NIWINSKA B,GRELA E R,et al.Effect of dietary glutamine,glucose and/or sodium butyrate on piglet growth,intestinal environment,subsequent fattener performance,and meat quality[J].Czech Journal of Animal Science,2014,59(10):460-470.

[22] 李宝成,王蕾,张俊梅,等.谷氨酰胺对断奶仔猪生长性能、血液生化指标及血浆氨基酸的影响[J].黑龙江畜牧兽医,2017(5):123-125.
[23] 马娅君,张小龙,陈庆菊,等.牛磺酸对早期断奶仔猪生长性能和肠道健康的影响[J].动物营养学报,2018,30(10):4121-4131.
[24] 穆勇攀,李愫,李新民.乳酸型复合酸化剂饲喂效果试验[J].畜牧兽医杂志,2011,30(6):7-8.
[25] WANG N F,CHEN Q,LE G W,et al.Effect of lactic acid fermented soyabean meal on the growth performance,intestinal microflora and morphology of weaned piglets[J].Journal of Animal and Feed Sciences,2007,16(1):75-85.

[26] 冯尚连,尚建钢,孙玥滢,等.乳酸对仔猪肠道大肠杆菌和乳酸杆菌的影响[J].浙江农业学报,2011,23(3):506-510.
[27] 姚继明,孟秀丽,吕锋,等.复合有机酸酸化剂对断奶仔猪生长性能及其肠道微生物菌群的影响[J].饲料工业,2017,38(2):11-16.
[28] HAN X Y,YAN F Y,NIE X Z,et al.Effect of replacing antibiotics using multi-enzyme preparations on production performance and antioxidant activity in piglets[J].Journal of Integrative Agriculture,2017,16(3):640-647.

[29] 李兆勇.玉米-豆粕型日粮添加复合酶制剂对仔猪生产性能影响及其机理研究[D].硕士学位论文.泰安:山东农业大学,2007.
[30] 徐春生,孙国君,蒋新环,等.复合酶制剂对仔猪生产性能和日粮养分消化率的影响[J].石河子大学学报(自然科学版),2004,22(1):60-62.
[31] 李梦云,张清海,赵武,等.液态复合添加剂对断奶1周内仔猪生长性能、养分消化率及血液指标的影响[J].中国畜牧杂志,2018,54(1):89-93.
[32] 巫丽娟,陈代文,毛湘冰,等.饮水中添加功能性复合添加剂对断奶仔猪生长性能、养分消化利用和血液指标的影响[J].畜牧兽医学报,2017,48(7):1365-1372.
[33] 巫丽娟,毛湘冰,余冰,等.饲粮添加功能性复合添加剂对生长猪生长性能和免疫功能的影响[J].动物营养学报,2017,29(5):1716-1722.
[34] 田辉,王可富,吴铁军.谷氨酰胺对MODS患者肠黏膜屏障功能影响的研究[J].中国急救医学,2006,26(10):708-709.
[35] 于新明,李虎,赵小刚,等.葡萄糖酸、谷氨酰胺及其组合物替代饲用抗生素对仔猪生产性能的影响[J].新疆农业科学,2012,49(1):139-145.
[36] 李欢,黄牛,何流琴,等.谷氨酰胺对脂多糖诱导的断奶仔猪氧化应激的影响[J].动物营养学报,2017,29(4):1350-1358.
[37] OKADA T,FUKUDA S,HASE K,et al.Microbiota-derived lactate accelerates colon epithelial cell turnover in starvation-refed mice[J].Nature Communications,2013,4(3):1654.
[38] 杨彩梅.谷氨酰胺和甘氨酰-谷氨酰胺对断奶仔猪小肠黏膜的影响[J].中国粮油学报,2006,21(4):119-123.
[39] 杨彩梅,陈安国.谷氨酰胺对早期断奶仔猪生产性能和小肠消化酶活性的影响[J].中国畜牧杂志,2005,41(6):21-22.
[40] 冯尚连,朱建津,孙玥莹,等.乳酸对仔猪消化酶发育的影响[J].浙江农业学报,2013,25(3):475-479.
[41] 李瑞珍.日粮中添加不同水平乳酸对断奶仔猪生产性能和有关生化指标的影响研究[D].硕士学位论文.郑州:河南农业大学,2009.
[42] HSU C B,HUANG H J,WANG C H,et al.The effect of glutamine supplement on small intestinal morphology and xylose absorptive ability of weaned piglets[J].African Journal of Biotechnology,2010,9(41):7003-7008.
[43] ZHONG X,ZHANG X H,LI X M,et al.Intestinal growth and morphology is associated with the increase in heat shock protein 70 expression in weaning piglets through supplementation with glutamine[J].Journal of Animal Science,2011,89(11):3634-3642.

[44] 晏家友.缓释复合酸化剂对断奶仔猪消化道酸度及肠道形态和功能的影响[D].硕士学位论文.雅安:四川农业大学,2009.
[45] LI S,ZHENG J,DENG K,et al.Supplementation with organic acids showing different effects on growth performance,gut morphology,and microbiota of weaned pigs fed with highly or less digestible diets[J].Journal of Animal Science,2018,96(8):3302-3318.
[46] GONG Z Y,YUAN Z Q,DONG Z W,et al.Glutamine with probiotics attenuates intestinal inflammation and oxidative stress in a rat burn injury model through altered iNOS gene aberrant methylation[J].American Journal of Translational Research,2017,9(5):2535-2547.

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