Effects of Short-Time Heat Stress on Growth Performance and Adenosine 5'-Monophosphate Activated Protein Kinase Signal Pathway of Broiler Chickens

WANG Jiguang; SUN Xiaolei; ZHANG Hui; SONG Zhigang; SU Pengcheng

doi:10.3969/j.issn.1006-267x.2019.12.019

2019 , Vol. 31 >Issue 12: 5533 - 5540

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.019

Effects of Short-Time Heat Stress on Growth Performance and Adenosine 5'-Monophosphate Activated Protein Kinase Signal Pathway of Broiler Chickens

WANG Jiguang ,
SUN Xiaolei ,
ZHANG Hui ,
SONG Zhigang ,
SU Pengcheng

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College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2019-05-08

Online published: 2019-12-13

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Abstract

This trial was conducted to investigate the effects of short-time heat stress on growth performance and adenosine 5'-monophosphate activated protein kinase (AMPK) signal pathway in appetite centers of broilers. Seventy-two 28-day-old Arbor Acres broilers with similar weight were randomly allocated into 2 groups (heat stress group and control group) with 6 replicates per group and 6 chicks per replicate. The ambient temperature in the heat stress group was (33±1)℃, and the ambient temperature in the control group was (25±1)℃, the experiment last for 10 h. At the end of the experiment, statistic growth performance of broilers, and the plasma and hypothalamus samples were collected from 1 chicken in each replicate. The contents of uric acid (UA), glucose (GLU), triglyceride (TG) and total cholesterol (TCHO) in plasma, and the mRNA relative expressions of cannabinoid 1 (CB1), serine/threonine-protein kinase 1 (AKT1), AMPKα1, AMPKα2, hepatokinase B1 (LKB1), mammalian target of rapamycin (mTOR) and glucocorticoid receptor (GR) in hypothalamus of broilers were detected, as well as the phosphorylation level of AMPK. The results showed as follows:1) compared with the control group, the feed intake and body weight gain of broilers in heat stress group were significantly decreased (P<0.05). 2) Compared with the control group, the contents of GLU, UA and TCHO in plasma of broilers in heat stress group were significantly increased (P<0.05). 3) Compared with the control group, the mRNA relative expressions of AMPKα1 and GR in hypothalamus of broilers in heat stress group were significantly decreased (P<0.05), the phosphorylation level of AMPK in hypothalamus of broilers in heat stress group was significantly decreased (P<0.05), and the mRNA relative expressions of LKB1, AKT1, mTOR, CB1 and AMPKα2 in hypothalamus of broilers in heat stress group had no significant difference (P>0.05). In conclusion, short-time heat stress can significantly decrease the growth performance and phosphorylation level of AMPK in hypothalamus of broilers, significantly decrease the mRNA relative expressions of AMPKα1 and GR in hypothalamus, and point out that AMPK signal pathway may be involved in the regulation of appetite inhibition by short-time heat stress in broilers.

Key words： short-time heat stress; growth performance; AMPK; appetite

Cite this article

WANG Jiguang , SUN Xiaolei , ZHANG Hui , SONG Zhigang , SU Pengcheng . Effects of Short-Time Heat Stress on Growth Performance and Adenosine 5'-Monophosphate Activated Protein Kinase Signal Pathway of Broiler Chickens[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5533 -5540 . DOI: 10.3969/j.issn.1006-267x.2019.12.019

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