Effects of Supplemented with L-Arginine or N-Carbamylglutamate on Plasma Metabolites before and after 3 600 m Speed Race of Yili Horses

ZHANG Shiqi; LI Xiaobin; ZHANG Wenjie; HAN Ming; WANG Shichang; YANG Kailun

doi:10.3969/j.issn.1006-267x.2019.12.027

2019 , Vol. 31 >Issue 12: 5613 - 5624

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.027

Effects of Supplemented with L-Arginine or N-Carbamylglutamate on Plasma Metabolites before and after 3 600 m Speed Race of Yili Horses

ZHANG Shiqi ,
LI Xiaobin ,
ZHANG Wenjie ,
HAN Ming ,
WANG Shichang ,
YANG Kailun

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Xinjiang Key Laboratory of Meat&Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2019-06-13

Online published: 2019-12-13

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Abstract

The aim of this study was to investigate the effects of supplemented with L-arginine (L-Arg) or N-carbamylglutamate (NCG) on plasma metabolites before and after of 3 600 m speed race of Yili horses. Fifteen Yili horses with similar body weight[(388.88±23.24) kg], aged 2 years old, were divided into 3 groups (5 horses in each group, 3♂+2♀) and trained according to the same training programs. Horses in control group were supplemented with 123 g/(horse·d) L-alanine (L-Ala), those in L-Arg group were supplemented with 60 g/(horse·d) L-Arg, and those in NCG group were supplemented with 12 g/(horse·d) NCG and 112 g/(horse·d). The experiment lasted for 4 weeks. The 3 600 m speed race was conducted at the 28th day of experiment, and samples were collected at pre-race 2 h, immediately post-race and post-race 2 h to analysis plasma metabolites by liquid chromatograph/mass spectrometer (LC/MS) technology. The results showed that, compared with the control group, the contents of plasma citric acid at pre-race 2 h, plasma α-ketoadipic acid at immediately post-race, and plasma D-glucose-6-phosphate at post-race 2 h in L-Arg group were significantly increased (P<0.05), and the content of plasma lactic acid was significantly decreased at immediately post-race (P<0.05); the content of plasma in NCG group was significantly decreased at immediately post-race (P<0.05). Compared with the control group, the contents of plasma arginine, acetylglutamic acid, glutamyl proline were significantly increased (P<0.05) and plasma glutamine content was significantly decreased in L-Arg group at pre-race 2 h (P<0.05), and the contents of plasma ornithine, acetylglutamic acid and proline were significantly increased at immediately post-race (P<0.05); the content of plasma arginine in NCG group was significantly increased at pre-race 2 h (P<0.05). Moreover, the contents of plasma leucine, tryptophan and 5-hydroxytryptophan in L-Arg group and NCG group were significantly increased at pre-race 2 h (P<0.05). In this experiment, the plasma differential expression metabolites before and after 3 600 m speed race of Yili horses supplemented with L-Arg or N-carbamylglutamic acid are screened by non-targeted metabonomics. It is revealed that supplemented with L-Arg or NCG can promote exercise energy metabolism, affect amino acid metabolism, promote the synthesis of arginine and the metabolism of arginine to proline in Yili horses.

Key words： Yili horses; L-arginine; N-carbamylglutamate; non-targeted metabolomics; plasma metabolites

Cite this article

ZHANG Shiqi , LI Xiaobin , ZHANG Wenjie , HAN Ming , WANG Shichang , YANG Kailun . Effects of Supplemented with L-Arginine or N-Carbamylglutamate on Plasma Metabolites before and after 3 600 m Speed Race of Yili Horses[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5613 -5624 . DOI: 10.3969/j.issn.1006-267x.2019.12.027

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