Effects of Dietary Protein Level on Immune and Antioxidant Capacity of Juvenile Schizopygopsis younghusbandi Regan

  • XU Zhaoli ,
  • ZENG Benhe ,
  • YANG Ruibin ,
  • MOU Zhenbo ,
  • LI Baohai ,
  • WANG Wanliang ,
  • ZHANG Bianbian ,
  • LIU Haiping
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  • 1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
    2. Institute of Fisheries Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China;
    3. Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China

Received date: 2019-06-13

  Online published: 2019-12-13

Abstract

This experiment was conducted to study the effects of dietary protein level on immune and antioxidant capacity of juvenile Schizopygopsis youughusbaudi Regan. A total of 540 healthy, uniform-sized, fully domesticated juvenile Schizopygopsis youughusbaudi Regan with body weight of (22.42±0.56) g were randomly divided into 6 groups with 3 replicates per group and 30 fish per replicate. Fish in each group were fed experimental diets that the protein levels were 20%, 25%, 30%, 35%, 40% and 45%, respectively. The experiment lasted for 60 days. The results showed as follows:1) the body weight growth rate of 30% and 35% protein level groups was significantly higher than that of other groups (P<0.05). 2) Dietary protein level had significant effects on the indexes of immune and antioxidant in serum and liver of juvenile Schizopygopsis youughusbaudi Regan (P<0.05). The lysozyme (LSZ), acid phosphatase (ACP), alkaline phosphatase (AKP), superoxide dismutase (SOD) and catalase (CAT) activities and immunoglobulin M (IgM) content in serum and activities of ACP, AKP, SOD and CAT in liver were showed a firstly increased and then decreased trend with dietary protein level increased, and the liver malondialdehyde (MDA) content was showed a firstly decreased and then stabilize trend with dietary protein level increased. The activities of immune enzymes LZM, ACP, AKP and antioxidant enzymes CAT, SOD in serum or liver and serum IgM content were the highest at 30% or 35% of dietary protein level, and the liver MDA content stabled after 35% of dietary protein level. In conclusion, considering the growth, immunity and antioxidant indexes, the polymorphic regression analysis shows that the suitable protein level of the artificial compound feed of (22.42±0.56) g juvenile Schizopygopsis youughusbaudi Regan is 30.43% to 33.88%.

Cite this article

XU Zhaoli , ZENG Benhe , YANG Ruibin , MOU Zhenbo , LI Baohai , WANG Wanliang , ZHANG Bianbian , LIU Haiping . Effects of Dietary Protein Level on Immune and Antioxidant Capacity of Juvenile Schizopygopsis younghusbandi Regan[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5645 -5654 . DOI: 10.3969/j.issn.1006-267x.2019.12.030

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