Effects of Molybdenum on Oxidative Damage and Cytoskeleton in Skeletal Muscle Epithelial Cells of Mouse

MA Shuhao; YANG Zijun; DU Boqiang; LI Guangzhao; ZHANG Cai; WANG Jicang; WANG Hongwei; KONG Tao

doi:10.3969/j.issn.1006-267x.2019.12.032

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 12: 5664 - 5671

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.032

Effects of Molybdenum on Oxidative Damage and Cytoskeleton in Skeletal Muscle Epithelial Cells of Mouse

MA Shuhao ,
YANG Zijun ,
DU Boqiang ,
LI Guangzhao ,
ZHANG Cai ,
WANG Jicang ,
WANG Hongwei ,
KONG Tao

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College of Animal Science and Technology, Henan University of Science and Technology, Henan Provincial Open Laboratory of Key Disciplines for Environmental and Animal Products Safety, Luoyang 471000, China

Received date: 2019-06-17

Online published: 2019-12-13

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Abstract

This experiment was conducted to investigate the effect of molybdenum (Mo) on oxidative damage and cytoskeleton in skeletal muscle epithelial cells of mouse. Used the primary skeletal muscle epithelial cells of mouse as materials, and cultured for 24 h in medium with Mo concentrations of 0 (group Ⅰ), 0.1 (group Ⅱ), 0.2 (group Ⅲ), 0.4 (group Ⅳ), and 0.8 mmol/L (group Ⅴ), respectively. Inverted microscope for cell morphology changes, methylthio tetrazole (MTT) method for cell viability, colorimetric assay for cell antioxidant capacity and oxidative damage, and quantitative real-time PCR (qRT-PCR) and Western-blot for Vimentin, α-actin and β-actin mRNA and protein expression. The results showed as follows:1) the morphology of cells in groups Ⅰ and Ⅱ was normal, cell gaps in groups Ⅲ, Ⅳ and Ⅴ were increased, cells were swollen, and cells in group Ⅴ showed obvious deformity. 2) Compared with the group Ⅰ, the viability in skeletal muscle epithelial cells of mouse in groups Ⅱ, Ⅲ, Ⅳ and Ⅴ was significantly decreased (P<0.01). 3) Compared with the group Ⅰ, the superoxide dismutase activity and total antioxidant capacity in skeletal muscle epithelial cells of mouse in groups Ⅱ, Ⅲ, Ⅳ and Ⅴ were significantly decreased (P<0.01), and the malondialdehyde content was significantly increased (P<0.01). 4) Compared with the group Ⅰ, the mRNA relative expressions of Vimentin, α-actin and β-actin in skeletal muscle epithelial cells of mouse in groups Ⅱ and Ⅲ were significantly increased (P<0.05), and that in group Ⅴ was significantly decreased (P<0.05); the protein relative expressions of Vimentin, α-actin and β-actin in skeletal muscle epithelial cells of mouse in groups Ⅲ, Ⅳ and Ⅴ were significantly decreased (P<0.05). In conclusion, after the skeletal muscle epithelial cells of mouse treated with high concentration of Mo, the morphology is abnormal and deformity, cells viability is decrease, the anti-oxidation ability is inhibited and the oxidative damage is aggravated, at the same time it can also inhibit the mRNA and protein expression of Vimentin, α-actin and β-actin.

Key words： molybdenum; skeletal muscle epithelial cells; oxidative damage; cytoskeletal protein

Cite this article

MA Shuhao , YANG Zijun , DU Boqiang , LI Guangzhao , ZHANG Cai , WANG Jicang , WANG Hongwei , KONG Tao . Effects of Molybdenum on Oxidative Damage and Cytoskeleton in Skeletal Muscle Epithelial Cells of Mouse[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5664 -5671 . DOI: 10.3969/j.issn.1006-267x.2019.12.032

References

[1] MENDEL R R.Cell biology of molybdenum[J].Biofactors,2009,35(5):429-434.

[2] GIRIDHARAN S S P,CAPLAN S.MICAL-family proteins:complex regulators of the actin cytoskeleton[J].Antioxidants & Redox Signalling,2014,20(13):2059-2073.

[3] STOURNARAS C,GRAVANIS A,MARGIORIS A N,et al.The actin cytoskeleton in rapid steroid hormone actions[J].Cytoskeleton,2014,71(5):285-293.

[4] 王亚垒,杨自军,张鹏,等.高钼对雄性绵羊骨骼X线特征及骨密度变化的影响[J].河南农业科学,2013,42(4):145-148.
[5] AUNG K H,TSUKAHARA S,MEAKAWA F,et al.Role of environmental chemical insult in neuronal cell death and cytoskeleton damage[J].Biological and Pharmaceutical Bulletin,2015,38(8):1109-1112.

[6] 孙宝飞,王恒,康朝胜,等.慢性砷中毒对骨骼肌超微结构的影响[J].黔南民族医专学报,2015,28(3):165-167,232.
[7] MUSARÒ A,CAROSIO S.Isolation and culture of satellite cells from mouse skeletal muscle[M]//DI NARDO P,DHINGRA S,SINGLA D.Adult stem cells.New York:Springer,2017.
[8] 孙素玲,刘明明,后家蘅.钼对大鼠致畸和致突变作用的研究[J].癌变·畸变·突变,2007,19(3):250-252.
[9] 杨平,惠起源.钼及其化合物配合物与胃癌研究进展[J].微量元素与健康研究,2012,29(1):65-67.
[10] YE N H,LIU S T,LIN Y Y,et al.Protective effects of intraperitoneal injection of TAT-SOD against focal cerebral ischemia/reperfusion injury in rats[J].Life Sciences,2011,89(23/24):868-874.
[11] CARILLON J,FOURET G,FEILLET-COUDRAY C,et al.Short-term assessment of toxicological aspects,oxidative and inflammatory response to dietary melon superoxide dismutase in rats[J].Food and Chemical Toxicology,2013,55:323-328.
[12] TAMAYO D,MUÑOZ J F,ALMEIDA A J,et al.Paracoccidioides spp.catalases and their role in antioxidant defense against host defense responses[J].Fungal Genetics and Biology,2017,100:22-32.
[13] DRAGUN Z,MARIJIC V F,KRASNICI N,et al.Malondialdehyde concentrations in the intestine and gills of Vardar chub (Squalius vardarensis Karaman) as indicator of lipid peroxidation[J].Environmental Science and Pollution Research,2017,24(20):16917-16926.

[14] 梁影.血清乳酸脱氢酶检测在巨幼细胞性贫血诊断中的意义[J].临床医学研究与实践,2016,1(22):32-33.
[15] SUN X R,SUN Z,ZHU Z,et al.Clinicopathological significance and prognostic value of lactate dehydrogenase A expression in gastric cancer patients[J].PLoS One,2014,9(3):91068.
[16] SHICHIRI M.The role of lipid peroxidation in neurological disorders[J].Journal of Clinical Biochemistry and Nutrition,2014,54(3):151-160.

[17] WANG Y,ZHAO H J,SHAO Y Z,et al.Copper (Ⅱ) and/or arsenite-induced oxidative stress cascades apoptosis and autophagy in the skeletal muscles of chicken[J].Chemosphere,2018,206:597-605.
[18] KOROBOVA F,RAMABHADRAN V,HIGGS H N.An actin-dependent step in mitochondrial fission mediated by the ER-associated formin INF2[J].Science,2013,339(6118):464-467.

[19] JIANG H K,QIU G R,LI L J,et al.Reduced ACTC1 expression might play a role in the onset of congenital heart disease by inducing cardiomyocyte apoptosis[J].Circulation Journal,2010,74(11):2410-2418.

[20] CHEN X L,ZHENG J,CAI J Y,et al.The cytoskeleton protein β-actin may mediate T cell apoptosis during acute rejection reaction after liver transplantation in a rat model[J].American Journal of Translational Research,2017,9(11):4888-4901.
[21] 李锦,王加丽,罗明秀,等.NIV毒素对软骨细胞骨架蛋白的影响及硒的拮抗作用[J].国外医学(医学地理分册),2013,34(3):146-150.
[22] WU D,HUANG C J,JIAO X F,et al.Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells[J].Oncotarget,2017,8(51):88630-88644.
[23] 韩婧,潘燕,李学军.波形蛋白的结构、功能和与肿瘤的关系[J].医学分子生物学杂志,2011,8(3):265-268.
[24] BARRIÈRE G,TARTARY M,RIGAUD M.Metformin:a rising star to fight the epithelial mesenchymal transition in oncology[J].Anticancer Agents Med Chem,2013,13(2):333-340.

[25] 刘丰彬,赵斌,闫万军,等.负重训练和补充大豆多肽干预大鼠骨骼肌衰老过程的实验研究[J].天津体育学院学报,2010,25(1):33-37.

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