Effects of Yeast Chromium and Diludin on Nutrient Digestibility and Blood Physiological and Biochemical Indicators of Heat Stressed Dairy Cows

GAO Jing; ZHAO Chuanchao; LI Han; WANG Yu; XU Hongyang; LI Rong; GAO Si; QI Zhili

doi:10.3969/j.issn.1006-267x.2019.12.038

2019 , Vol. 31 >Issue 12: 5719 - 5729

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.038

Effects of Yeast Chromium and Diludin on Nutrient Digestibility and Blood Physiological and Biochemical Indicators of Heat Stressed Dairy Cows

GAO Jing ,
ZHAO Chuanchao ,
LI Han ,
WANG Yu ,
XU Hongyang ,
LI Rong ,
GAO Si ,
QI Zhili

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1. College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
2. Shangdong Dezhou Shenniu Animal Pharmaceutical Co., Ltd., Dezhou 253074, China;
3. Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi 445000, China

Received date: 2019-05-21

Online published: 2019-12-13

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Abstract

The object of this experiment was to evaluate the effects of yeast chromium and diludin on nutrient digestibility and blood physiological and biochemical indicators of heat stressed dairy cows. Twenty-four lactating Holstein cows with similar parity, body condition and lactation stage were randomly assigned to 3 groups, including control group (basal diet), yeast chromium group[basal diet supplemented with yeast chromium 80 mg/(head·d)] and diludin group[basal diet supplemented with diludin 3 g/(head·d)], 8 cows each group. The experiment had a 6-day adaption period, following a 46-day experimental period. Daily temperature-humidity index (THI) during trial were higher than 72. Digestibility trial was conducted on days 23 to 27 and blood samples were collected at different THI (76.7, 77.5, 78.0, 81.0, 88.1, 85.7 and 78.9) to measure the heat shock protein 70 (HSP70) mRNA expression level and serum the contents of triiodothyronine (T₃) and tetraiodothyronine (T₄), the activities of alkaline phosphatase (ALP) and creatine kinase (CK), the concentrations of potassium ions (K⁺), sodion ions (Na⁺), chloride ion (Cl^-), and electrolyte equilibrium value. The results showed as follows:1) compared with control group, yeast chromium and diludin both significantly increased dry matter intake (DMI) and the digestibility of crude protein (CP) (P<0.05), while yeast chromium also significantly increased the digestibility of dry matter (DM) and neutral detergent fiber (NDF) (P<0.05). 2) Compared with control group, yeast chromium trended to upregulate the mean of blood HSP70 mRNA expression level (P=0.06) and significantly increased the blood HSP70 mRNA expression level when THI were 81.0 and 88.1 (P<0.05); diludin also trended to upregulate the mean of blood HSP70 mRNA expression level (P=0.09) and significantly increased the blood HSP70 mRNA expression level when THI were 81.0 and 85.7 (P<0.05). 3) Yeast chromium and diludin did not affect the contents of T₃, T₄ and the activity of ALP in serum (P>0.05), but significantly decreased serum CK activity compared with control group when THI were 77.5 and 88.1 (P<0.05). 4) Compared with control group, when THI was 81.0, yeast chromium and diludin both significantly increased serum K⁺ concentration (P<0.05); when THI was 78.0, yeast chromium significantly increased serum Na⁺ concentration (P<0.05); diludin had no effects on serum Na⁺ concentration (P>0.05); yeast chromium and diludin had no effects on serum Cl^- concentration (P>0.05); when THI was 78.0, yeast chromium significantly increased serum electrolyte equilibrium value (P<0.05); diludin had no effect on serum electrolyte equilibrium value (P>0.05). In conclusion, yeast chromium and diludin can improve the feed intake and nutrient digestibility, decrease the serum CK activity, maintain the electrolyte equilibrium balance and relieve the heat stress of heat stressed dairy cows.

Key words： yeast chromium; diludin; heat stress; nutrient digestibility; blood physiological and biochemical indicators

Cite this article

GAO Jing , ZHAO Chuanchao , LI Han , WANG Yu , XU Hongyang , LI Rong , GAO Si , QI Zhili . Effects of Yeast Chromium and Diludin on Nutrient Digestibility and Blood Physiological and Biochemical Indicators of Heat Stressed Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5719 -5729 . DOI: 10.3969/j.issn.1006-267x.2019.12.038

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