Effects of Lactobacillus plantarum and It Mixed with Urea Added in Whole Corn Silage on Digestion and Metabolism, Serum Biochemical Indexes and Rumen Fermentation Indexes of Dorper Sheep

CHENG Guangmin; CHEN Fengmei; ZHANG Yongcui; LIU Gongyan; YANG Lei; CHEN Guihua; XU Xiangting

doi:10.3969/j.issn.1006-267x.2019.12.039

2019 , Vol. 31 >Issue 12: 5730 - 5739

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.039

Effects of Lactobacillus plantarum and It Mixed with Urea Added in Whole Corn Silage on Digestion and Metabolism, Serum Biochemical Indexes and Rumen Fermentation Indexes of Dorper Sheep

CHENG Guangmin ,
CHEN Fengmei ,
ZHANG Yongcui ,
LIU Gongyan ,
YANG Lei ,
CHEN Guihua ,
XU Xiangting

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1. Shandong Vocational Animal Science and Veterinary College, Weifang 261061, China;
2. Animal Husbandry and Veterinary Institute, Shandong Academy of Agricultural Sciences, Ji'nan 251000, China;
3. Inner Mongolia Autonomous Region Hailar District Chenbaerhuqiba Town Agriculture and Animal Husbandry Service Center, Hulun Buir 021000, China

Received date: 2019-05-06

Online published: 2019-12-13

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Abstract

This experiment was conducted to study the effects of Lactobacillus plantarum and it mixed with urea added in whole corn silage on digestion and metabolism, serum biochemical and rumen fermentation indexes of Dorper sheep, to provide scientific basis for better utilization of whole corn silage fattening sheep. Sixty healthy male Dorper sheep in fattening period with similar body weight[(23.0±1.5) kg] were selected and randomly divided into 3 groups with 4 replicates per group and 5 sheep per replicate. Sheep were fed with no additives whole corn silage (control group), single addition of 0.1% Lactobacillus plantarum of whole corn fresh weight (probiotics group) and 0.1% Lactobacillus plantarum mixed with 0.5% urea mixture of whole corn fresh weight (mixed group) formulated total mixed rations, respectively. The pre-experimental period lasted for 10 days, and the experimental period lasted for 90 days. The results showed as follows:1) the intakes of crude protein (CP), crude ash (Ash), calcium (Ca) and neutral washing fiber (NDF) of mixed group were significantly higher than those of probiotics group and control group (P<0.05). The nitrogen intake (IN), digestible nitrogen (DN), retention nitrogen (RN) and nitrogen digestibility (NAD) and nitrogen utilization ratio (NUR) of mixed group were significantly higher than those of the control group (P<0.05), and the apparent digestibilities of Ash and NDF were significantly higher than those of the control group (P<0.05). 2) The albumin (ALB), triglyceride (TG) contents and alanine aminotransferase (ALT), glutamate aminotransferase (AST) activities in serum of probiotics group were significantly higher than those of the control group (P<0.05), and the serum γ-glutamyltransferase (γ-GGT) activity was significantly lower than that of the control group (P<0.05). The serum TG content of mixed group was significantly higher than that of the control group (P<0.05), and the serum γ-GGT activity was significantly lower than that of the control group (P<0.05). 3) The rumen fluid ammonia nitrogen content of mixed group was significantly higher than that of probiotics group and control group (P<0.05); the contents of acetic acid and total volatile fatty acid (TVFA) in rumen fluid of probiotics group were significantly higher than those of the control group (P<0.05), and the pH was significantly lower than that of the control group (P<0.05); the rumen fluid butyric acid content of mixed group was significantly lower than that of probiotics group and control group (P<0.05). In conclusion, the single addition of Lactobacillus plantarum in whole corn silage total mixed ration cannot change the digestion and metabolism indexes of Dorper sheep, and the addition of Lactobacillus plantarum mixed with urea in whole corn silage total mixed ration is conducive to the digestion and absorption of nitrogen, and affect the serum biochemical indexes and rumen fermentation indexes of Dorper sheep.

Key words： Lactobacillus plantarum; whole corn silage; Dorper sheep; digestion and metabolism; serum biochemical; rumen fermentation

Cite this article

CHENG Guangmin , CHEN Fengmei , ZHANG Yongcui , LIU Gongyan , YANG Lei , CHEN Guihua , XU Xiangting . Effects of Lactobacillus plantarum and It Mixed with Urea Added in Whole Corn Silage on Digestion and Metabolism, Serum Biochemical Indexes and Rumen Fermentation Indexes of Dorper Sheep[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5730 -5739 . DOI: 10.3969/j.issn.1006-267x.2019.12.039

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