Effects of Fermented Feed on Laying Performance, Egg Quality, Intestinal Digestive Enzyme Activities and Immune Function of Laying Ducks

RUAN Dong; LIU Jiangao; CHEN Wei; WANG Shang; ZHANG Ya; CHENG Aihua; XIA Weiguang; JIANG Shouqun; LU Lizhi; ZHENG Chuntian

doi:10.3969/j.issn.1006-267x.2019.12.040

2019 , Vol. 31 >Issue 12: 5740 - 5749

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.040

Effects of Fermented Feed on Laying Performance, Egg Quality, Intestinal Digestive Enzyme Activities and Immune Function of Laying Ducks

RUAN Dong ,
LIU Jiangao ,
CHEN Wei ,
WANG Shang ,
ZHANG Ya ,
CHENG Aihua ,
XIA Weiguang ,
JIANG Shouqun ,
LU Lizhi ,
ZHENG Chuntian

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1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science(South China) of Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
2. New Hope Liuhe Limited Liability Company Guangdong New District, Guangzhou 511480, China;
3. Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310012, China

Received date: 2019-05-23

Online published: 2019-12-13

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Abstract

This experiment was conducted to investigate the effects of dietary different levels of fermented mixing feed and fermented distiller's grains on laying performance, egg quality, intestinal digestive enzyme activities, and immune function of laying ducks. Seven hundred and fifty 35-week-old Longyan laying ducks were randomly assigned to 5 groups with6 replicates per group and 25 ducks per replicate. Ducks in control group were fed a basal diet, and ducks in experimental groups were fed the basal diet supplemented with 2%, 4% fermented mixing feed and 2%, 4% fermented distiller's grains, respectively. The experiment lasted for 3 months. The results showed as follows:compared with the control group, dietary 2% and 4% fermented distiller's grains significantly increased the daily egg mass of laying ducks (P<0.05), dietary 4% fermented distiller's grains significantly decreased the feed to egg of laying ducks (P<0.05), dietary 4% fermented distiller's grains significantly increased the albumin weight and yolk color in egg (P<0.05), dietary 4% fermented mixing feed and 4% fermented distiller's grains significantly decreased the pH in gizzard and glandular stomach of laying ducks (P<0.05), dietary 4% fermented mixing feed significantly increased the jejunal lipase and chymotrypsin activities of laying ducks (P<0.05), dietary 2% and 4% fermented distiller's grains significantly increased the jejunal chymotrypsin and trypsin activities of laying ducks (P<0.05), and dietary 4% fermented distiller's grains significantly increased the jejuna mucin 2 and secretory immunoglobulin A (SIgA) mRNA relative expression and SIgA content of laying ducks (P<0.05). In conclusion, fermented distiller's grains is superior to fermented mixing feed, it can improve the laying performance, egg quality, intestinal digestive enzyme activities and immune function of laying ducks, and the effect of 4% supplemental level is better.

Key words： fermented feed; laying duck; laying performance; digestive enzyme activities; intestinal immunity

Cite this article

RUAN Dong , LIU Jiangao , CHEN Wei , WANG Shang , ZHANG Ya , CHENG Aihua , XIA Weiguang , JIANG Shouqun , LU Lizhi , ZHENG Chuntian . Effects of Fermented Feed on Laying Performance, Egg Quality, Intestinal Digestive Enzyme Activities and Immune Function of Laying Ducks[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5740 -5749 . DOI: 10.3969/j.issn.1006-267x.2019.12.040

References

[1] CHENG G Y,HAO H H,XIE S Y,et al.Antibiotic alternatives:the substitution of antibiotics in animal husbandry?[J].Frontiers in Microbiology,2014,5:217.
[2] GAO J,ZHANG H J,WU S G,et al.Effect of Saccharomyces cerevisiae fermentation product on immune functions of broilers challenged with Eimeria tenella[J].Poultry Science,2009,88(10):2141-2151.

[3] FENG J,LIU X,XU Z R,et al.Effects of Aspergillus oryzae 3.042 fermented soybean meal on growth performance and plasma biochemical parameters in broilers[J].Animal Feed Science and Technology,2007,134(3/4):235-242.
[4] 吕月琴,孙汝江,肖发沂,等.微生物发酵饲料对蛋鸡肠道菌群和氮磷排泄率的影响[J].家禽科学,2012(6):9-11.
[5] 崔卫涛,况世昌,李钢平,等.生物发酵饲料对蛋鸡生产性能和鸡蛋品质的影响[J].畜牧与饲料科学,2018,39(10):33-36.
[6] FENG J,LIU X,XU Z R,et al.Effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers[J].Poultry Science,2007,86(6):1149-1154.

[7] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method[J].Methods,2001,25(4):402-408.

[8] GU C Q,XIE C Q,HU X Y,et al.Cytokine gene expression in the livers of ducklings infected with duck hepatitis virus-1 JX strain[J].Poultry Science,2012,95(3):583-591.
[9] ZHANG Q,EICHER S D,APPLEGATE T J.Development of intestinal mucin 2,IgA,and polymeric Ig receptor expressions in broiler chickens and Pekin ducks[J].Poultry Science,2015,94(2):172-180.

[10] 余勃,游金明,陆豫,等.固态发酵菜粕替代日粮中豆粕对肉仔鸡生长性能的影响[J].动物营养学报,2009,21(2):239-244.
[11] JAZI V,BOLDAJI F,DASTAR B,et al.Effects of fermented cottonseed meal on the growth performance,gastrointestinal microflora population and small intestinal morphology in broiler chickens[J].British Poultry Science,2017,58(4):402-408.

[12] 王少琨,朱子晨,张倩,等.发酵酒糟对海兰褐蛋鸡生产性能、蛋品质和肠道健康的影响[J].中国家禽,2018,40(9):30-33.
[13] ENGBERG R M,HAMMERSHØJ M,JOHANSEN N F,et al.Fermented feed for laying hens:effects on egg production,egg quality,plumage condition and composition and activity of the intestinal microflora[J].British Poultry Science,2009,50(2):228-239.

[14] OKRATHOK S,PASRI P,THONGKRATOK R,et al.Effects of cassava pulp fermented with Aspergillus oryzae as a feed ingredient substitution in laying hen diets[J].The Journal of Applied Poultry Research,2018,27(2):188-197.

[15] 周建民,马友彪,张海军,等.白酒糟酵母培养物对肉仔鸡生长性能、血清抗氧化指标和肠道形态结构的影响[J].动物营养学报,2019,31(5):2357-2366.
[16] RABOT S,RAFTER J,RIJKERS G T,et al.Guidance for substantiating the evidence for beneficial effects of probiotics:impact of probiotics on digestive system metabolism[J].The Journal of Nutrition,2010,140(3):677S-689S.
[17] CAPCAROVA M,WEISS J,HRNCAR C,et al.Effect of Lactobacillus fermentum and Enterococcus faecium strains on internal milieu,antioxidant status and body weight of broiler chickens[J].Journal of Animal Physiology and Animal Nutrition,2010,94(5):e215-e224.
[18] 李乐,张敏,耿春银,等.不同添加量的富硒乳酸菌对蛋鸡免疫和抗氧化性能的影响[J].中国饲料,2017(19):21-24.
[19] 张彩凤,齐广海,王晓翠,等.乳酸菌和酵母菌复合制剂及其与维吉尼亚霉素联合使用对肉仔鸡血清生化指标、抗氧化能力和免疫功能的影响[J].动物营养学报,2018,30(5):1893-1901.
[20] 李士平.蛋黄着色影响因素及着色剂的研究[D].硕士学位论文.哈尔滨:东北农业大学,2000.
[21] 吴俊锋,詹凯,曹君平,等.枯草芽孢杆菌及其发酵豆粕对蛋鸡生产性能、蛋品质及血清生化指标的影响[J].中国家禽,2012,34(1):22-26.
[22] LEI K,LI Y L,YU D Y,et al.Influence of dietary inclusion of Bacillus licheniformis on laying performance,egg quality,antioxidant enzyme activities,and intestinal barrier function of laying hens[J].Poultry Science,2013,92(9):2389-2395.

[23] TAYLOR W H.Studies on gastric proteolysis.4.Proteinase activity of gastric juice and gastric-mucosal extracts at pH 6 to 8[J].Biochemical Journal,1959,71(4):626-632.

[24] JIN L Z,HO Y W,ABDULLAH N,et al.Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures[J].Poultry Science,2000,79(6):886-891.

[25] 石琳,张少成,陈广信,等.不同营养水平饲粮添加酵母浓缩物对罗斯肉鸡生长性能、肠道消化酶活性及肠黏膜组织结构的影响[J].中国兽医学报,2018,38(1):201-205.
[26] 岳晓敬,扶雄锋,胡栾莎,等.复合益生菌发酵豆粕对断奶仔猪肠道形态和消化酶活性的影响[J].中国畜牧杂志,2016,52(11):49-54.
[27] 袁文华,李国勤,赵文文,等.益生菌对乳鸽生长性能、血清生化指标及肠道功能的影响[J].动物营养学报,2018,30(11):4721-4728.
[28] MACPHERSON A J,MCCOY K D,JOHANSEN F E,et al.The immune geography of IgA induction and function[J].Mucosal Immunology,2008,1(1):11-22.

[29] HAPFELMEIER S,LAWSON M A E,SLACK E,et al.Reversible microbial colonization of germ-free mice reveals the dynamics of IgA immune responses[J].Science,2010,328(5986):1705-1709.

[30] LINDEN S K,SUTTON P,KARLSSON N G,et al.Mucins in the mucosal barrier to infection[J].Mucosal Immunology,2008,1(3):183-197.

[31] 刘辉,季海峰,王四新,等.副干酪乳杆菌发酵饲料对生长猪生长性能、粪便菌群数量与挥发性脂肪酸含量以及血清生化和免疫指标的影响[J].动物营养学报, 2019,31(8):3747-3754.
[32] ROOKS M G,GARRETT W S.Gut microbiota,metabolites and host immunity[J].Nature Reviews Immunology,2016,16(6):341-352.

[33] SHEN T,WANG G C,YOU L,et al.Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice[J].Food & Nutrition Research,2017,61(1):1344523.
[34] 王文文,王园,郝希然,等.发酵麸皮多糖对大鼠组织细胞因子含量及盲肠菌群结构的影响[J].动物营养学报,2019,31(6):2865-2874.
[35] SÁNCHEZ B,GUEIMONDE M,PEÑA A S,et al.Intestinal microbiota as modulators of the immune system[J].Journal of Immunology Research,2015,2015:159094.
[36] APAJALAHTI J,VIENOLA K.Interaction between chicken intestinal microbiota and protein digestion[J].Animal Feed Science and Technology,2016,221:323-330.

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