Effects of Dietary Different Concentrations of Tetracycline on Growth Performance, Intestinal Microbiota Composition and Morphology of Channa argus

  • MIAO Shuyan ,
  • HAN Bei ,
  • HU Juntao ,
  • ZHU Jinyu ,
  • ZHANG Xin ,
  • DONG Xiaojing ,
  • CHEN Guohong
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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2019-06-03

  Online published: 2019-12-13

Abstract

In the study of nutrient metabolism related to intestinal microecology, antibiotics were usually used to inhibit the growth of intestinal microbiota, in order to study the relationship between intestinal microbiota and host nutrient metabolism, but the effects of antibiotics on the nutritional metabolism and intestinal health of experimental animals were often ignored. This experiment was aimed to investigate the effect of dietary different concentrations of tetracycline on growth performance, intestinal microbiota composition and morphology of Channa argus, to provide reference for related research. A total of 360 strong and healthy juvenile Channa argus with similar body weight[(9.43±0.12) g] were randomly divided into 3 groups with 4 replicates per group and 4 fish per replicate. Fish in the control group (group D1) were fed a basal diet, and others in experimental groups (groups D2 and D3) were fed basal diets supplemented with 0.5 and 1.0 mg/kg tetracycline, respectively. The experiment lasted for 30 days. The results showed as follows:1) the weight gain rate, specific growth rate and survival rate of groups D2 and D3 were significantly lower than those of group D1 (P<0.05), the feed conversion ratio of group D3 was significantly higher than that of group D1 (P<0.05). 2) The intestinal operational taxonomic units (OTU) number of D3 was significantly lower than that of group D1 (P<0.05). The Sobs index, ACE index and Shannon index of groups D2 and D3 were significantly lower than those of group D1 (P<0.05), and the Simpson index was significantly higher than that of group D1 (P<0.05). 3) Dietary different concentrations of tetracycline decreased the proportion of intestinal Firmicute, Bacillaceae, Pseudononadaceae, Lactococcus, Geobacillus and Bacillus, but increased the proportion of Proteobacteria, Streptococcaceae, Moraxellaceae, Streptococcus, Leuconostoc and Acinetobacter. 4) The intestinal villus height of groups D1 was significantly higher than that of groups D2 and D3 (P<0.05), and the intestinal wall thickness and villus width of group D3 was significantly lower than that of group D1 (P<0.05). In conclusion, long-term (30 d) tetracycline stress can result in the microecological disorder and serious damage of intestinal tissue, which further cause the weight gain rate, specific growth rate and survival rate of Channa argus decrease, and feed conversion ratio increase.

Cite this article

MIAO Shuyan , HAN Bei , HU Juntao , ZHU Jinyu , ZHANG Xin , DONG Xiaojing , CHEN Guohong . Effects of Dietary Different Concentrations of Tetracycline on Growth Performance, Intestinal Microbiota Composition and Morphology of Channa argus[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5813 -5822 . DOI: 10.3969/j.issn.1006-267x.2019.12.047

References

[1] GÓMEZ G D,BALCÁZAR J L.A review on the interactions between gut microbiota and innate immunity of fish[J].FEMS Immunology & Medical Microbiology,2008,52(2):145-154.  
[2] MORIYA T,SATOMI Y,MURATA S,et al.Effect of gut microbiota on host whole metabolome[J].Metabolomics,2017,13(9):101.
[3] FLINT H J,SCOTT K P,LOUIS P,et al.The role of the gut microbiota in nutrition and health[J].Nature Reviews Gastroenterology & Hepatology,2012,9(10):577-589.  
[4] MERRIFIELD D L,OLSEN R E,MYKLEBUST R,et al.Dietary effect of soybean (Glycine max) products on gut histology and microbiota of fish[M]//EL-SHEMY H A.Soybean and nutrition.Rijeka:InTech,2011.
[5] CANI P D,POSSEMIERS S,VAN DE WIELE T,et al.Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability[J].Gut,2009,58(8):1091-1103.  
[6] KEMPER N.Veterinary antibiotics in the aquatic and terrestrial environment[J].Ecological Indicators,2008,8(1):1-13.  
[7] SEYFRIED E E,NEWTON R J,RUBERT Ⅳ K F,et al.Occurrence of tetracycline resistance genes in aquaculture facilities with varying use of oxytetracycline[J].Microbial Ecology,2010,59(4):799-807.  
[8] SHENG Y,REN H,LIMBU S M,et al.The presence or absence of intestinal microbiota affects lipid deposition and related genes expression in zebrafish (Danio rerio)[J].Frontiers in Microbiology,2018,9:1124.
[9] HE S,ZHOU Z,MENG K,et al.Effects of dietary antibiotic growth promoter and Saccharomyces cerevisiae fermentation product on production,intestinal bacterial community,and nonspecific immunity of hybrid tilapia (Oreochromis niloticus female×Oreochromis aureus male)[J].Journal of Animal Science,2011,89(1):84-92.  
[10] BARROS-BECKER F,ROMERO J,PULGAR A,et al.Persistent oxytetracycline exposure induces an inflammatory process that improves regenerative capacity in zebrafish larvae[J].PLoS One,2012,7(5):e36827.
[11] ZHOU L,LIMBU S M,QIAO F,et al.Influence of long-term feeding antibiotics on the gut health of zebrafish[J].Zebrafish,2018,15(4):340-348.  
[12] ZHOU L,LIMBU S M,SHEN M L,et al.Environmental concentrations of antibiotics impair zebrafish gut health[J].Environmental Pollution,2018,235:245-254.
[13] STRAUS D L,BOWKER J D,BOWMAN M P,et al.Safety of aquaflor-medicated feed to sunshine bass[J].North American Journal of Aquaculture,2012,74(1):1-7.  
[14] MUTIYAR P K,MITTAL A K.Risk assessment of antibiotic residues in different water matrices in India:key issues and challenges[J].Environmental Science and Pollution Research,2014,21(12):7723-7736.  
[15] YIRUHAN,WANG Q J,MO C H,et al.Determination of four fluoroquinolone antibiotics in tap water in Guangzhou and Macao[J].Environmental Pollution,2010,158(7):2350-2358.  
[16] NIE X P,LIU B Y,YU H J,et al.Toxic effects of erythromycin,ciprofloxacin and sulfamethoxazole exposure to the antioxidant system in Pseudokirchneriella subcapitata[J].Environmental Pollution,2013,172:23-32.
[17] YAN Z H,LU G H,YE Q X,et al.Long-term effects of antibiotics,norfloxacin,and sulfamethoxazole,in a partial life-cycle study with zebrafish (Danio rerio):effects on growth,development,and reproduction[J].Environmental Science and Pollution Research,2016,23(18):18222-18228.  
[18] 佟建明.饲用抗生素的促生长作用机制及其替代技术研究[J].饲料工业,2006,27(2):1-4.
[19] TULSTRUP M V L,CHRISTENSEN E G,CARVALHO V,et al.Antibiotic treatment affects intestinal permeability and gut microbial composition in Wistar rats dependent on antibiotic class[J].PLoS One,2015,10(12):e0144854.
[20] HILL D A,HOFFMANN C,ABT M C,et al.Metagenomic analyses reveal antibiotic-induced temporal and spatial changes in intestinal microbiota with associated alterations in immune cell homeostasis[J].Mucosal Immunology,2010,3(2):148-158.  
[21] 周文豪,陈孝煊,陈昌福.投喂氯霉素和土霉素后草鱼肠道菌群变化[J].华中农业大学学报,1997, 25(增刊):91-100.
[22] UYAGUARI M,KEY P,MOORE J,et al.Acute effects of the antibiotic oxytetracycline on the bacterial community of the grass shrimp,Palaemonetes pugio[J].Environmental Toxicology and Chemistry,2009,28(12):2715-2724.  
[23] NAVARRETE P,MARDONES P,OPAZO R,et al.Oxytetracycline treatment reduces bacterial diversity of intestinal microbiota of Atlantic salmon[J].Journal of Aquatic Animal Health,2008,20(3):177-183.  
[24] PANDA S,EL KHADER I,CASELLAS F,et al.Short-term effect of antibiotics on human gut microbiota[J].PLoS One,2014,9(4):e95476.
[25] 杨雨辉,佟恒敏,卢彤岩,等.乳酸环丙沙星对鲤鱼肠道菌群的影响[J].中国兽医杂志,2003,39(10):38-40.
[26] DIBNER J J,RICHARDS J D.Antibiotic growth promoters in agriculture:history and mode of action[J].Poultry Science,2005,84(4):634-643.  
[27] SANG H M,FOTEDAR R.Effects of mannan oligosaccharide dietary supplementation on performances of the tropical spiny lobsters juvenile (Panulirus ornatus,Fabricius 1798)[J].Fish & Shellfish Immunology,2010,28(3):483-489.  
[28] 谷琳琳,姜海龙,王鹏,等.添加抗生素对动物肠道健康的影响[J].养猪,2015(3):125-128.
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