Determination of Tocopherol and Tocotrienol Contents in Natural Vitamin E of Feed Additive by Normal Phase Liquid Chromatography

WANG Shi; WANG Jitong; WANG Yanni; XIAO Zhiming; JIA Zheng; LI Yang; LIU Xiaolu; FAN Xia

doi:10.3969/j.issn.1006-267x.2019.12.048

2019 , Vol. 31 >Issue 12: 5823 - 5833

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.048

Determination of Tocopherol and Tocotrienol Contents in Natural Vitamin E of Feed Additive by Normal Phase Liquid Chromatography

WANG Shi ,
WANG Jitong ,
WANG Yanni ,
XIAO Zhiming ,
JIA Zheng ,
LI Yang ,
LIU Xiaolu ,
FAN Xia

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1. Institute of Quality Standards&Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Beijing Feed Testing Institute, Beijing 100107, China;
3. Chinese Academy of Agricultural Mechanization Sciences, Beijing 100083, China

Received date: 2019-05-24

Online published: 2019-12-13

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Abstract

The natural vitamin E has been gradually used in pets and livestock breeding because of it can promote the growth of animals and improve the meat quality. Nowadays, there are many kinds of feed additives containing natural vitamin E sold in the markets. However, the standard analytical methods have not yet been developed to monitor these products. In this work, an analytical method using normal phase liquid chromatography-ultraviolet and visible spectrophotometry (NPLC-UV) had been developed for the simultaneous determination of eight natural vitamin E contents. The samples were wetted with methanol and ultrasonic extracted using ethyl acetate/hexane (3:97, V/V). The target analytes were successfully separated on a Kromasil 60-5-diol column (4.6 mm×150 mm) with the mobile phase consisting of N-heptane/Tetrahydrofuran (100:4,V/V) at a flow rate of 1.0 mL/min. The column was thermostated at 40℃ and UV detection was performed at 292 nm. Good linearity was obtained in the ranges of 0.2 to 50 μg/mL, with linear coefficients (R²) higher than 0.999 0. The limit of detection (LOD) was 0.01%. Average recoveries from the fortification samples ranged between 81.3% and 109.8%, with relative standard deviations (RSD) lower than 7.9%. By analyzing the actual sample, the proposed method is reliable, sensitive and easy to perform, making it applicable for routine analysis to provide technical support for market regulation.

Key words： feed additive; natural vitamin E; tocopherol; tocotrienol; normal phase liquid chromatography

Cite this article

WANG Shi , WANG Jitong , WANG Yanni , XIAO Zhiming , JIA Zheng , LI Yang , LIU Xiaolu , FAN Xia . Determination of Tocopherol and Tocotrienol Contents in Natural Vitamin E of Feed Additive by Normal Phase Liquid Chromatography[J]. Chinese Journal of Animal Nutrition, 2019 , 31(12) : 5823 -5833 . DOI: 10.3969/j.issn.1006-267x.2019.12.048

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