Effects of Citrus Extract on Immunity of Piglets

CUI Yiyan; TIAN Zhimei; DENG Dun; WANG Gang; LU Huijie; CHEN Weidong; MA Xianyong

doi:10.3969/j.issn.1006-267x.2020.01.015

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 1: 99 - 108

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.015

Effects of Citrus Extract on Immunity of Piglets

CUI Yiyan ,
TIAN Zhimei ,
DENG Dun ,
WANG Gang ,
LU Huijie ,
CHEN Weidong ,
MA Xianyong

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Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Key Laboratory of Animal Breeding and Nutrition, The Key Laboratory of Animal Nutrition and Feed Science(South China) of Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2019-07-18

Online published: 2020-01-19

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Abstract

This experiment was conducted to investigate the effects of citrus extract on immune function in serum, liver, duodenal and jejunal mucosa of piglets. One hundred and forty-four 28-day-old (8.39±1.06) kg Duroc×Landrace×Large White pigs were randomly assigned to 3 groups with 6 pens (replicates) per group and 8 pigs per pen (half male and half female). Three groups were:control group, fed a basal diet; antibiotic group, fed the basal diet supplemented with 75 g/t chlortetracycline; citrus extract group, fed the basal diet supplemented with 300 mL/t citrus extract. The experiment lasted for 28 days. The results showed as follows. 1) compared with the control group, the serum immunoglobulin G (IgG) content of piglets in the antibiotic group and the citrus extract group was significantly increased by 11.27% and 14.01%, respectively (P<0.05); compared with the antibiotic group and the control group, the contents of serum interleukin-6 (IL-6) and immunoglobulin G (IgA) of piglets in the citrus extract group were tended to be increased (0.05 ≤ P < 0.10); the serum tumor necrosis factor-α (TNF-α) content of piglets in the citrus extract group was significantly increased by 12.13% compared with the control group (P<0.05), but not significantly different from that in the antibiotic group (P>0.05). 2) Compared with the control group, the contents of liver immunoglobulin M and IgG of piglets in the citrus extract group and the antibiotic group were significantly decreased (P<0.05). Compared with the control group and the citrus extract group, the liver TNF-α content of piglets in the antibiotic group was significantly decreased (P<0.05), and liver IgA content had a decreasing trend (0.05 ≤ P < 0.10). 3) There was no significant difference in indices of duodenal mucosal immunity among three groups (P>0.05). 4) Compared with the control group, the citrus extract and antibiotic had a trend to increase contents of interleukin-2 and IL-6 in jejunal mucosa of piglets (0.05 ≤ P < 0.10), and the IL-8 content in jejunal mucosa of piglets in the antibiotic group was significantly increased (P<0.05). The secretory immunoglobulin A content in jejunal mucosa of piglets in the citrus extract group was the highest, which was 19.35% higher than that in the control group (0.05 ≤ P < 0.10). In brief, citrus extract and antibiotic can improve the contents of cytokines and immunoglobulins in serum and jejunal mucosa, thus enhance the immunity of piglets.

Key words： citrus extract; piglets; immunity

Cite this article

CUI Yiyan , TIAN Zhimei , DENG Dun , WANG Gang , LU Huijie , CHEN Weidong , MA Xianyong . Effects of Citrus Extract on Immunity of Piglets[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 99 -108 . DOI: 10.3969/j.issn.1006-267x.2020.01.015

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