Effects of Natural and Synthetic Humic Acids on Performance, Serum Gonadotropin Concentrations, Liver Antioxidant Capacity and Intestinal Morphological Structure of Laying Hens under Heat Stress

LU Xintao; QIU Jialing; ZHU Jiaming; ZHOU Qin; LIU Bing; YU Dongyou

doi:10.3969/j.issn.1006-267x.2020.01.023

2020 , Vol. 32 >Issue 1: 180 - 188

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.023

Effects of Natural and Synthetic Humic Acids on Performance, Serum Gonadotropin Concentrations, Liver Antioxidant Capacity and Intestinal Morphological Structure of Laying Hens under Heat Stress

LU Xintao ,
QIU Jialing ,
ZHU Jiaming ,
ZHOU Qin ,
LIU Bing ,
YU Dongyou

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Key Laboratory of Animal Nutrition and Feed in East China of Ministry of Agriculture and Rural Affairs, College of Animal Science, Zhejiang University, Hangzhou 310058, China

Received date: 2019-07-01

Online published: 2020-01-19

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Abstract

This study was conducted to explore the effects of dietary natural and synthetic humic acids (HAs) on performance, serum gonadotropin concentrations, liver antioxidant capacity and intestinal morphological structure of laying hens under heat stress. Five hundred and forty healthy Hy-Line White laying hens of 60-week-old with similar body weight of[(1.32±0.24) kg] and consistent laying rate of[(80.16±0.21)%] were randomly divided into 4 groups with 9 replicates per group and 15 hens per replicate. Laying hens in control group 1 were fed a basal diet at normal temperature[(25±1)℃], and the others were fed a basal diet (control group 2) and the basal diets supplemented with 1 000 mg/kg natural humic acids (group humic acids A) and 1 000 mg/kg synthetic humic acids (group humic acids B) at high temperature[(35±1)℃]. The experiment lasted for 4 weeks. The results show as follows:1) compared with control group 1, laying rate (LR), average daily feed intake (ADFI), serum luteinizing hormone (LH) concentration, jejunum villus height (V) and the ratio of villus height to crypt depth (V/C) and ileum V of laying hens in control group 2 were significantly decreased (P<0.01), and unqualified eggs rate (UER), mortality, liver malondialdehyde (MDA) content and catalase (CAT) activity were significantly increased (P<0.01). Compared with control group 2, dietary natural humic acids significantly increased LR, serum LH concentration, jejunum V and V/C and ileum V of haying hens (P<0.01), and significantly decreased UER, liver MDA content and CAT activity (P<0.01). In conclusion, dietary humic acids can increase antioxidant capacity, regulate serum gonadotropin concentrations, improve performance and alleviate intestine injury caused by heat stress of laying hens, and the effects of natural humic acids is better in this experiment.

Key words： humic acids; heat stress; laying hens; laying performance; gonadotropins; antioxidant capacity; intestinal morphology

Cite this article

LU Xintao , QIU Jialing , ZHU Jiaming , ZHOU Qin , LIU Bing , YU Dongyou . Effects of Natural and Synthetic Humic Acids on Performance, Serum Gonadotropin Concentrations, Liver Antioxidant Capacity and Intestinal Morphological Structure of Laying Hens under Heat Stress[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 180 -188 . DOI: 10.3969/j.issn.1006-267x.2020.01.023

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