Effects of Increasing Ruminal Acetate and Butyrate Production Rate on Gastrointestinal Passage and Absorption in Sheep

DU Ruiping; LU Dexun; GAO Min

doi:10.3969/j.issn.1006-267x.2020.01.028

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 1: 224 - 233

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.028

Effects of Increasing Ruminal Acetate and Butyrate Production Rate on Gastrointestinal Passage and Absorption in Sheep

DU Ruiping ,
LU Dexun ,
GAO Min

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Animal Nutrition Institute of Agriculture and Animal Husbandry Academy of Inner Mongolia, Huhhot 010031, China

Received date: 2019-07-17

Online published: 2020-01-19

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Abstract

This experiment was conducted to study the effects of increasing production rate of ruminal acetate and butyrate on their gastrointestinal absorption and passage. Six healthy Inner Mongolian semifine-wool wethers (1.5-old-year, similar body weight) fitted with a ruminal and a duodenum cannula were randomly divided into two groups (acetate infusion group and butyrate infusion group) with three sheep in each group. Each group set 3 treatments, each treatment was an infusion level of acetate/butyrate and a pulse-continuous infusion experiment. Rumen steady state was obtained by simulating continuous feeding, and non-isotope labeled acetate and butyrate solutions technology was used. The results showed as follows:1) ruminal pH and acetate fractional absorption rate decreased significantly (P<0.05) and ruminal acetate concentration, acetate absorption rate and acetate passage rate increased significantly (P<0.01) with increasing ruminal acetate production rate. Meanwhile, ruminal Co concentration, ruminal liquid hour dilution rate, ruminal liquid flow rate and liquid volume changed insignificantly with increasing ruminal acetate production rate (P>0.05). Except ruminal Co concentration decreased significantly (P<0.05) with increasing ruminal butyrate production rate, other indexes change were as the same as acetate infusion. 2) Acetate duodenum passage rate increased insignificantly (2.95 to 3.47 mmol/h) with increasing ruminal acetate production rate (P>0.05), the percentage of duodenum acetate entering hind-tract (5.68% to 3.35%) and acetate absorption percentage in the omasum and abomasum (94.32% to 96.65%) increased significantly (P<0.01) with increasing ruminal acetate production rate. In this study, increasing ruminal acetate and butyrate production rate in sheep increase ruminal concentration of acetate and butyrate, decrease ruminal pH, increase ruminal absorption rate of acetate and butyrate and passage rate in rumen and duodenum, and decrease ruminal absorption efficiency of acetate and butyrate.

Key words： sheep; rumen; acetate; butyrate; production; absorption; passage

Cite this article

DU Ruiping , LU Dexun , GAO Min . Effects of Increasing Ruminal Acetate and Butyrate Production Rate on Gastrointestinal Passage and Absorption in Sheep[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 224 -233 . DOI: 10.3969/j.issn.1006-267x.2020.01.028

References

[1] MOLONEY A P.Growth and carcass composition in sheep offered isoenergetic rations which resulted in different concentrations of ruminal metabolites[J].Livestock Production Science,1998,56(2):157-164.

[2] JANSSEN P H.Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics[J].Animal Feed Science and Technology,2010,160(1/2):1-22.
[3] KOHN R A,BOSTON R C.The role of thermodynamics in controlling rumen metabolism[C]//MCNAMARA J P,FRANCE J,BEEVER D E.Modelling nutrient utilization in farm animals.Wallingford,UK:CABI Publishing.
[4] BERGMAN E N.Energy contributions of volatile fatty acids from the gastrointestinal tract in various species[J].Physiological Reviews,1990,70(2):567-590.

[5] LÍPEZ S,HOVELL F D D,DIJKSTRA J,et al.Effects of volatile fatty acid supply on their absorption and on water kinetics in the rumen of sheep sustained by intragastric infusions[J].Journal of Animal Science,2003,81(10):2609-2616.

[6] 熊本海.生长肥育羊瘤胃内VFA产生、吸收规律及模型参数研究[D].博士学位论文.北京:中国农业科学院,1998.
[7] DIEHO K,DIJKSTRA J,SCHONEWILLE J T,et al.Changes in ruminal volatile fatty acid production and absorption rate during the dry period and early lactation as affected by rate of increase of concentrate allowance[J].Journal of Dairy Science,2016,99(7):5370-5384.

[8] 杜瑞平,卢德勋,高民.绵羊瘤胃乙酸和丁酸的产生、吸收和流通规律及可代谢生脂物质的测定[J/OL].动物营养学报:1-11[2019-09-18].http://kns.cnki.net/kcms/detail/11.5461.s.20190912.1910.018.html.
[9] 杜瑞平.绵羊瘤胃乙酸和丁酸的产生、吸收和利用规律及可代谢生脂物质(MLS)的测定[D].硕士学位论文.呼和浩特:内蒙古农业大学,2006.
[10] NRC.Nutrient Requirements of sheep[S].Washington,D.C.:National Academy Press,1985.
[11] UDEN P,COLUCCI P E,VAN SOEST P J.Investigation of chromium,cerium and cobalt as markers in digesta.Rate of passage studies[J].Journal of the Science of Food and Agriculture,1980,31(7):625-632.

[12] KOLVER E S,DE VETH M J.Prediction of ruminal pH from pasture-based diets[J].Journal of Dairy Science,2002,85(5):1255-1266.

[13] PENNER G B,ASCHENBACH J R,GÄBEL G,et al.Epithelial capacity for apical uptake of short chain fatty acids is a key determinant for intraruminal pH and the susceptibility to subacute ruminal acidosis in sheep[J].The Journal of Nutrition,2009,139(9):1714-1720.

[14] DIJKSTRA J,ELLIS J L,KEBREAB E,et al.Ruminal pH regulation and nutritional consequences of low pH[J].Animal Feed Science and Technology,2012,172(1/2):22-33.
[15] ASCHENBACH J R,PENNER G B,STUMPFF F,et al.Ruminant nutrition symposium:role of fermentation acid absorption in the regulation of ruminal pH[J].Journal of Animal Sicence,2011,89(4):1092-1107.
[16] 封贵.瘤胃内灌注乙酸对山羊瘤胃内环境及真胃壁M₂、M₃和eNOS的基因表达影响[D].硕士学位论文.扬州:扬州大学,2016.
[17] PETERS J P,SHEN R Y W,ROBINSON J A,et al.Disappearance and passage of propionic acid from the rumen of the beef steer[J].Journal of Animal Science,1990,68(10):3337-3349.

[18] PETERS J P,SHEN R Y,ROBINSON J A.Disappearance of acetic acid from the bovine reticulorumen at basal and elevated concentrations of acetic acid[J].Journal of Animal Science,1992,70(5):1509-1517.

[19] STORM A C,HANIGAN M D,KRISTENSEN N B.Effects of ruminal ammonia and butyrate concentrations on reticuloruminal epithelial blood flow and volatile fatty acid absorption kinetics under washed reticulorumen conditions in lactating dairy cows[J].Journal of Dairy Science,2011,94(8):3980-3994.

[20] STORM A C,KRISTENSEN N B.Effects of particle size and dry matter content of a total mixed ration on intraruminal equilibration and net portal flux of volatile fatty acids in lactating dairy cows[J].Journal of Dairy Science,2010,93(9):4223-4238.

[21] DIJKSTRA J,BOER H,VAN BRUCHEM J,et al.Absorption of volatile fatty acids from the rumen of lactating dairy cows as influenced by volatile fatty acid concentration,pH and rumen liquid volume[J].British Journal of Nutrition,1993,69(2):385-396.

[22] PENNER G B,STEELE M A,ASCHENBACH J R,et al.Ruminant nutrition symposium:molecular adaptation of ruminal epithelia to highly fermentable diets[J].Journal of Animal Sicence,2011,89(9):1108-1119.
[23] SCHURMANN B L,WALPOLE M E,GÍRKA P,et al.Short-term adaptation of the ruminal epithelium involves abrupt changes in sodium and short-chain fatty acid transport[J].American Journal of Physiology.Regulatory,Integrative and Comparative Physiology,2014,307(7):R802-R816.
[24] GRAHAM C,GATHERAR I,HASLAM I,et al.Expression and localization of monocarboxylate transporters and sodium/proton exchangers in bovine rumen epithelium[J].American Journal of Physiology.Regulatory,Integrative and Comparative Physiology,2007,292(2):R997-R1007.
[25] BUGAUT M.Occurrence,absorption and metabolism of short chain fatty acids in the digestive tract of mammals[J].Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,1987,86(3):439-472.

[26] MELO L Q,COSTA S F,LOPES F,et al.Rumen morphometrics and the effect of digesta pH and volume on volatile fatty acid absorption[J].Journal of Animal Science,2013,91(4):1775-1783.

[27] SUTTON J D,DHANOA M S,MORANT S V,et al.Rates of production of acetate,propionate,and butyrate in the rumen of lactating dairy cows given normal and low-roughage diets[J].Journal of Dairy Science,2003,86(11):3620-3633.

[28] ALUWONG T,KOBO P,ABDULLAHI A.Volatile fatty acids production in ruminants and the role of monocarboxylate transporters:a review[J].African Journal of Biotechnology,2010,9(38):6229-6232.
[29] KIRAT D,MATSUDA Y,YAMASHIKI N,et al.Expression,cellular localization,and functional role of monocarboxylate transporter 4(MCT4) in the gastrointestinal tract of ruminants[J].Gene,2007,391(1/2):140-149.
[30] CONNOR E E,LI R W,BALDWIN R L,et al.Gene expression in the digestive tissues of ruminants and their relationships with feeding and digestive processes[J].Animal,2010,4(7):993-1007.

[31] GÄBEL P,BESTMANN M,MARTENS H.Influences of diet,short-chain fatty acids,lactate and chloride on bicarbonate movement across the reticulo-rumen wall of sheep[J].Journal of Veterinary Medicine Series A,1991,38(1/2/3/4/5/6/7/8/9/10):523-529.
[32] 李文.瘤胃液pH值、渗透压、挥发性脂肪酸(VFAs)浓度对绵羊瘤胃上皮VFAs吸收影响的研究[D].硕士学位论文.泰安:山东农业大学,2014.
[33] EDRISE B M,SMITH R H.Absorption of volatile fatty acids in different compartments of the ruminant stomach[J].Proceeding of the Nutrition Society,1977,36(3):149A.
[34] 赵玮.舍饲羊皱胃组织形态学研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2010.
[35] 吴美玲.1.5岁阿尔巴斯绒山羊在不同饲养条件下瓣胃与皱胃形态变化的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2005.
[36] FRANCE J,DIJKSTRA J.Volatile fatty acid production[M]//DIJKSTRA J,FORBES J M,FRANCE J.Quantitative aspects of ruminant digestion and metabolism.Wallingford,UK:CABI Publishing,2005:157-175.
[37] MARKANTONATOS X,GREEN M H,VARGA G A.Use of compartmental analysis to study ruminal volatile fatty acid metabolism under steady state conditions in Holstein heifers[J].Animal Feed Science and Technology,2008,143(1/2/3/4):70-88.

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