Effects of Dietary Non-Fiber Carbohydrate/Neutral Detergent Fiber on Nutrient Digestion and Rumen Bacterial Structure of Karakul Sheep

PU Xuanxuan; GUO Xuefeng; JIANG Chenyu; LIU Junfeng; ZHANG Xiuping; ZHANG Sujiang

doi:10.3969/j.issn.1006-267x.2020.01.035

2020 , Vol. 32 >Issue 1: 285 - 294

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.035

Effects of Dietary Non-Fiber Carbohydrate/Neutral Detergent Fiber on Nutrient Digestion and Rumen Bacterial Structure of Karakul Sheep

PU Xuanxuan ,
GUO Xuefeng ,
JIANG Chenyu ,
LIU Junfeng ,
ZHANG Xiuping ,
ZHANG Sujiang

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1. College of Animal Science, Tarim University, Alar 843300, China;
2. Key Laboratory of Tarim Animal Husbandry Science and Technology, Alar 843300, China

Received date: 2019-07-09

Online published: 2020-01-19

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Abstract

The aim of the experiment was to investigate effects of dietary non-fiber carbohydrate/neutral detergent fiber (NFC/NDF) on nutrient digestibility, bacterial structure of Karakul sheep. Twelve 18-month-old Karakul sheep with healthy condition, similar weight[(35.0±3.3) kg] and permanent fistula were randomly divided into four groups, each group with three replicates. They were fed four diets with different NFC/NDF at 0.54 (group 1), 0.96 (group 2), 1.37 (group 3) and 1.90 (group 4), respectively. The experiment was designed for four periods, periods Ⅰ (1 to 26 d), Ⅱ (27 to 52 d), Ⅲ (53 to 78 d) and Ⅳ (79 to 104 d). During each period, the first 15 days was used for adaption, and the trial period was 11 days including 8 days for digestion text, and 3 days for rumen digesta collection. The apparent digestibility of nutrients and structure of bacteria were measured for four periods. Results showed as follows:1) through four periods, the apparent digestibility of dry matter (DM) in groups 3 and 4 was significantly higher than that in groups 1 and 2 (P<0.05), the apparent digestibility of organic matter (OM) in groups 3 and 4 was significantly higher than that in groups 1 and 2 (P<0.05) in period Ⅰ, while the difference among groups was not significant in other periods (P>0.05), and the apparent digestibility of NDF and acid detergent fiber (ADF) in group 3 was the highest but the difference was not significant (P>0.05). 2) Through four periods, the difference of bacterial structure between groups 2 and 1, 3, 4 was larger, and the distribution of bacteria structure was relatively concentrated in the same diet under different periods. The relative abundance of cellulose-degrading bacteria, semi-cellulose-degrading bacteria and starch-degrading bacteria genus reached the highest in groups 2, 3 and 4, respectively. In conclusion, the digestibility of nutrients is improved when dietary NFC/NDF is 1.37; the bacteria structure changes largely when dietary NFC/NDF is 0.96 and the structure of rumen bacteria in the same group does not change much with the prolong of experimental periods; through four periods, the relative abundance of cellulose-degrading bacteria, semi-cellulose-degrading bacteria and starch-degrading bacteria at genus level is the highest when NFC/NDF are 0.96, 1.37 and 1.90, respectively.

Key words： NFC/NDF; apparent digestibility; bacteria; Karakul sheep

Cite this article

PU Xuanxuan , GUO Xuefeng , JIANG Chenyu , LIU Junfeng , ZHANG Xiuping , ZHANG Sujiang . Effects of Dietary Non-Fiber Carbohydrate/Neutral Detergent Fiber on Nutrient Digestion and Rumen Bacterial Structure of Karakul Sheep[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 285 -294 . DOI: 10.3969/j.issn.1006-267x.2020.01.035

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