Effects of Lactobacillus plantarum Supplementation on Growth and Intestinal Microbial Flora Diversity of Brachymystax lenok

HU Zongfu; NIU Huaxin; YU Jianhua; LI Shuguo; CHANG Jie

doi:10.3969/j.issn.1006-267x.2020.01.041

2020 , Vol. 32 >Issue 1: 346 - 356

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.041

Effects of Lactobacillus plantarum Supplementation on Growth and Intestinal Microbial Flora Diversity of Brachymystax lenok

HU Zongfu ,
NIU Huaxin ,
YU Jianhua ,
LI Shuguo ,
CHANG Jie

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1. College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China;
2. Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, Tongliao 028000, China

Received date: 2019-06-11

Online published: 2020-01-19

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Abstract

This experiment aimed to investigate the effects of Lactobacillus plantarum (L. plantarum) supplementation on the growth and intestinal microbial flora diversity of Brachymystax lenok (B. lenok). A basal diet was used in control group, while the diet of trial group was added L. plantarum in a dose of 1×10⁶ CFU/g based on the basal diet. Each group had 3 replicates and each replicate cultured 25 B. lenok (average body weight:5 to 8 g). After indoor feeding for 56 days, the contents and mucosa of mid and hind intestine were sampled, and used for microorganism DNA extraction. Then, the intestinal microbial flora diversity was detected for high-throughput sequencing technique. The results showed that L. plantarum supplementation significantly increased the specific growth rate (SGR) compared the control group (P<0.05). The α diversity analysis showed that the diversity (Shannon and Simpson indexes) and richness (Ace and Chao indexes) of intestinal microbial flora of trial group was significantly lower than those of the control group (P<0.05), and the β diversity analysis revealed the obvious separation of intestinal microbial flora from trial group and control group. At phylum level, the abundance of Firmicutes in the trial group was 96.03%, while the control group was composed of several phyla, they were Firmicutes (23.79%), Cyanobacteria (23.30%), Bacteroidetes (8.95%), Spirochaetae (6.67%) and Actinobacteria (5.35%). At genus level, there were only 3 genera with abundance>1% in the trial group, they were Lactobacillus (58.94%), Exiguobacterium (26.00%) and Enterococcus (10.62%), occupied for 95.66% of all intestinal microbial flora. While in the control group, there were 11 genera with abundance>1% and occupied for 58.24% of all intestinal microbial flora, such as unclassified_Cyanobacteria (23.30%), Ralstonia (11.70%), Brevinema (6.67%) and Prevotella (3.90%). It is concluded that L. plantarum supplementation improves the growth of B. lenok, decreases the diversity of intestinal microbial flora, increases the proportion of Lactobaccilus and inhibits the growth of other bacteria.

Key words： Brachymystax lenok; Lactobacillu plantarum; intestinal microbial flora; high-throughput sequencing

Cite this article

HU Zongfu , NIU Huaxin , YU Jianhua , LI Shuguo , CHANG Jie . Effects of Lactobacillus plantarum Supplementation on Growth and Intestinal Microbial Flora Diversity of Brachymystax lenok[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 346 -356 . DOI: 10.3969/j.issn.1006-267x.2020.01.041

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