Study on Preparation and in Vitro Digestive Characteristics of Lactobacillus acidophilus-Bifidobacterium Probiotics Microcapsules Feed by Composite Method

WU Xiaoman; HU Yueqin

doi:10.3969/j.issn.1006-267x.2020.01.043

2020 , Vol. 32 >Issue 1: 372 - 381

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.043

Study on Preparation and in Vitro Digestive Characteristics of Lactobacillus acidophilus-Bifidobacterium Probiotics Microcapsules Feed by Composite Method

WU Xiaoman ,
HU Yueqin

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National Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

Received date: 2019-07-22

Online published: 2020-01-19

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Abstract

In this paper, sodium alginate and chitosan were used as shell-materials, the Lactobacillus acidophilus, Bifidobacterium and feed were coated to preparing probiotic microcapsules feed by perforation-curing with complex coacervation composite method, and the preparation conditions were studied. In this experiment, the encapsulation rate was used as the main index to evaluate the probiotic microcapsule feed, and its particle size, suspension rate and solubility rate were also determined, and the effects of different factors on these physical and chemical properties were studied by single factor test and orthogonal test. The results showed that the optimum preparation process conditions were determined as:the sodium alginate concentration 1.25%, the proportion of probiotics and sodium alginate 1:10, calcium chloride concentration 1.5%, curing time 15 min, and chitosan concentration 1.5%. Under these conditions, the particle size of probiotic microcapsule feed is uniform, ranging from 1.60 to 2.00 mm; and the encapsulation rate is reached 96.68%. Moreover, the suspension rate is 77.96% and the solubility is 13.04% after soaking in water for 12 h; and still have a survival rate of 5.2 lg(CFU/mL) after 120 min in simulated artificial gastric juice; and the embedded live bacteria can be completely released after 120 min in simulated artificial intestinal fluid, which has good floatability, water stability, acid resistance and enteric solubility.

Key words： Lactobacillus acidophilus; Bifidobacterium; perforation-curing method; complex coacervation method; probiotic microcapsules feed

Cite this article

WU Xiaoman , HU Yueqin . Study on Preparation and in Vitro Digestive Characteristics of Lactobacillus acidophilus-Bifidobacterium Probiotics Microcapsules Feed by Composite Method[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 372 -381 . DOI: 10.3969/j.issn.1006-267x.2020.01.043

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