Changes of Physiological Constants, Serum Acute Phase Response Biomarkers and Redox Indexes of Canines after Ice Water Environment Stress and Subsequent Recovery

HE Shaojun; SONG Kai; WU Mingming; YIN Qirun; XIONG Yongjie; LI Jing

doi:10.3969/j.issn.1006-267x.2020.01.044

2020 , Vol. 32 >Issue 1: 382 - 389

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.044

Changes of Physiological Constants, Serum Acute Phase Response Biomarkers and Redox Indexes of Canines after Ice Water Environment Stress and Subsequent Recovery

HE Shaojun ,
SONG Kai ,
WU Mingming ,
YIN Qirun ,
XIONG Yongjie ,
LI Jing

Expand

College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China

Received date: 2019-07-04

Online published: 2020-01-19

Supported by

Fold

Abstract

This study was conducted to investigate the changes of physiological constants, serum acute phase response biomarkers and redox indicators of canines after ice water environment stress and subsequent recovery. Eight healthy Chinese rural dogs were selected. The body temperature, heart rate and respiratory rate were measured and 2 mL blood was taken from the jugular vein before ice water environment stress, at the end of ice water environment stress (after 0.5 h in ice water) and recovery 0.5, 1.0, 3.0, and 7.0 h after the ice water environment stress, respectively. Serum was separated and the activities or contents of acute phase reaction biomarkers and redox substances in serum were determined. The results showed as follows:compared with before ice water environment stress, the body temperature, heart rate and serum heat shock protein 70 (HSP70), cortisol contents and catalase activity were significantly decreased at the end of ice water environment stress (P<0.05), while the serum uric acid, carbonyl and malondialdehyde contents were significantly increased (P<0.05). The heart rate, serum vitamin E, total carbonyl and malondialdehyde contents returned to the level of before ice water environment stress after 0.5 h of ice water environment stress (P ≥ 0.05). The body temperature, serum cortisol, sulfhydryl, uric acid contents and ceruloplasmin activity recovered to the level of before ice water environment stress after 1.0 h of ice water environment stress (P ≥ 0.05), while the serum vitamin C content returned to before ice water environment stress level after 3.0 h of ice water environment stress (P ≥ 0.05). Compared with the end of ice water environment stress, the body temperature, heart rate, serum cortisol and vitamin E contents increased significantly (P<0.05), while the respiratory rate, serum total superoxide dismutase, catalase activities and HSP70, uric acid, total carbonyl, malondialdehyde contents decreased significantly when recovery 7.0 h after the ice water environment stress (P<0.05). In conclusion, ice water environment stress can significantly change the physiological constants, serum stress biomarker contents and redox status of canines. Some indicators are still abnormal during the recovery period, which suggested that the clinical measures should be taken to keep warm and supplement antioxidants for controlling the hazards of ice water environment stress.

Key words： canines; ice water environment; physiological constants; acute-phase response biomarkers; redox

Cite this article

HE Shaojun , SONG Kai , WU Mingming , YIN Qirun , XIONG Yongjie , LI Jing . Changes of Physiological Constants, Serum Acute Phase Response Biomarkers and Redox Indexes of Canines after Ice Water Environment Stress and Subsequent Recovery[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 382 -389 . DOI: 10.3969/j.issn.1006-267x.2020.01.044

References

[1] 赵恩军,华修国,张斌,等.冷热刺激对犬血清皮质醇、促肾上腺皮质激素及血液生理指标的影响[J].畜牧兽医学报,2003,34(5):457-460.
[2] PEAKE J M,ROBERTS L A,FIGUEIREDO V C,et al.The effects of cold water immersion and active recovery on inflammation and cell stress responses in human skeletal muscle after resistance exercise[J].The Journal of Physiology,2016,595(3):695-711.
[3] TRUXTON T T,MILLER K C.Can temperate-water immersion effectively reduce rectal temperature in exertional heat stroke?A critically appraised topic[J].Journal of Sport Rehabilitation,2017,26(5):447-451.

[4] 李云章,于江江,尹文文,等.丹参对冷应激犬心功能的保护作用研究[C]//中国畜牧兽医学会2008年学术年会暨第一届中国兽医临床大会论文集.广州:中国畜牧兽医学会,2009:390-396.
[5] 李静辉,张雪,孟宇,等.CIRP对冷应激小鼠血清生化指标、相关细胞免疫因子及能量代谢的影响[J].中国兽医学报,2016,36(1):132-136,142.
[6] VENDITTI P,DI STEFANO L,DI MEO S.Vitamin E reduces cold-induced oxidative stress in rat skeletal muscle decreasing mitochondrial H₂O₂ release and tissue susceptibility to oxidants[J].Redox Report,2009,14(4):167-175.

[7] 许友卿,郑一民,丁兆坤.冷应激对水生动物代谢的影响及调控研究进展[J].中国水产科学,2017,24(5):1149-1159.
[8] OHASHI Y,KATSUTA T,TANI H,et al.Human cold stress of strong local-wind "Hijikawa-arashi" in Japan,based on the UTCI index and thermo-physiological responses[J].International Journal of Biometeorology,2018,62(7):1241-1250.

[9] CHOO H C,NOSAKA K,PEIFFER J J,et al.Ergogenic effects of precooling with cold water immersion and ice ingestion:a meta-analysis[J].European Journal of Sport Science,2018,18(2):170-181.

[10] NELSON D H,EGDAHL R H,HUME D M.Corticosteroid secretion in the adrenal vein of the non-stressed dog exposed to cold[J].Endocrinology,1956,58(3):309-314.

[11] PALAZZOLO D L,QUADRI S K.Plasma thyroxine and cortisol under basal conditions and during cold stress in the aging dog[J].Experimental Biology and Medicine,1987,185(3):305-311.

[12] KAPAKIN K A T,KAPAKIN S,KUTLU Z,et al.Are there any differences in the expression of heat shock protein 70 in the liver of the rats exposed to cold stress in terms of gender?[J].Pakistan Veterinary Journal,2016,36(1):59-62.
[13] HEJAZI M M,BACHA A O,KALEEMUDDIN M,et al.Alteration of serum immunoglobins,C-reactive protein,vitamin D,and electrolyte by atenolol and amlodipine in stress-induced hypertensive rats[J].Molecular and Cellular Biochemistry,2018,445(1/2):99-103.
[14] AKHALAYA M Y,PLATONOV A G,BAIZHUMANOV A A.Short-term cold exposure improves antioxidant status and general resistance of animals[J].Bulletin of Experimental Biology and Medicine,2006,141(1):26-29.

[15] DUGUÉ B,SMOLANDER J,WESTERLUND T,et al.Acute and long-term effects of winter swimming and whole-body cryotherapy on plasma antioxidative capacity in healthy women[J].Scandinavian Journal of Clinical and Laboratory Investigation,2005,65(5):395-402.

[16] SUTKOWY P,WOŹ NIAK A,BORACZYŃ SKI T,et al.Oxidation-reduction processes in ice swimmers after ice-cold water bath and aerobic exercise[J].Cryobiology,2015,70(3):273-277.

[17] LUBKOWSKA A,DOŁEGOWSKA B,SZYGUŁA Z,et al.Winter-swimming as a building-up body resistance factor inducing adaptive changes in the oxidant/antioxidant status[J].Scandinavian Journal of Clinical and Laboratory Investigation,2013,73(4):315-325.

[18] REN J Y,LIU C P,ZHAO D,et al.The role of heat shock protein 70 in oxidant stress and inflammatory injury in quail spleen induced by cold stress[J].Environmental Science and Pollution Research,2018,25(21):21011-21023.

[19] SIEMS W G,VAN KUIJK F J G M,MAASS R,et al.Uric acid and glutathione levels during short-term whole body cold exposure[J].Free Radical Biology and Medicine,1994,16(3):299-305.

[20] YANG X,LUO Y H,ZENG Q F,et al.Effects of low ambient temperatures and dietary vitamin C supplement on growth performance,blood parameters,and antioxidant capacity of 21-day-old broilers[J].Poultry Science,2014,93(4):898-905.

[21] ZHANG Z W,LV Z H,LI J L,et al.Effects of cold stress on nitric oxide in duodenum of chicks[J].Poultry Science,2011,90(7):1555-1561.

[22] 王一璇.冷应激和冷适应游泳对大鼠心脑ATP酶、钙离子及自由基代谢的影响[D].硕士学位论文.大连:大连医科大学,2011:11-13.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References