A Colorimetric Method for Rapid and Quantitative Determination of Amino Acid Decarboxylase Activity and Its Application

CHEN Qingju; DENG Huan; HU Hong; LU Changwen; TANG Zhiru

doi:10.3969/j.issn.1006-267x.2020.01.045

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 1: 390 - 396

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.045

A Colorimetric Method for Rapid and Quantitative Determination of Amino Acid Decarboxylase Activity and Its Application

CHEN Qingju ,
DENG Huan ,
HU Hong ,
LU Changwen ,
TANG Zhiru

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Key Laboratory for Bio-Feed and Animal Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China

Received date: 2019-07-04

Online published: 2020-01-19

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Abstract

This study was designed to investigate a colorimetric method for rapid and quantitative detection of amino acid decarboxylase activity. The experiment used the decarboxylase standard to prepare the standard curve, and then used the absorbance value and the standard curve of the sample to calculate the amino acid decarboxylase activity of the sample. The amino acid decarboxylase activity of pig colonic microorganism and brain tissue samples was determined by the colorimetric method for detecting amino acid decarboxylase activity established in this experiment. The accuracy of the method was verified by measuring the amino acid decarboxylase activity of unknown samples. The results showed as follows:the standard curve with good linear relationship of the amino acid decarboxylase activity established by the tyrosine decarboxylase standard was:y=0.478 9x+0.112 6, R²=0.998 0. The standard curves of different amino acid decarboxylase activities had a high similarity to the standard curve of tyrosine decarboxylase activity, indicating that the method had wide applicability to various amino acid decarboxylase activities. The amino acid decarboxylase activity in pig colonic microorganism and brain tissue was rapidly determined by the colorimetric method established in this experiment. Moreover, the amino acid decarboxylase activity of the unknown sample measured by this method fell within the range of the established standard curve, indicating that the method had high accuracy. The results indicate that the method established in this experiment is a rapid method for detecting the activity of amino acid decarboxylase. It is lower in cost, simpler in operation, lower in requirements on instruments, reliable in method, and suitable for determination of amino acid decarboxylase activity in various samples. It can provide an effective assay for studying the activity of amino acid decarboxylase in animal tissues, microbial culture fluids, cell cultures and blood samples.

Key words： amino acid decarboxylase activity; microorganism; quantitative detection method

Cite this article

CHEN Qingju , DENG Huan , HU Hong , LU Changwen , TANG Zhiru . A Colorimetric Method for Rapid and Quantitative Determination of Amino Acid Decarboxylase Activity and Its Application[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 390 -396 . DOI: 10.3969/j.issn.1006-267x.2020.01.045

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