Regulating Effect of Natural Bee Bead and Bee Pollen on Immune Function of Mice

LI Zhen; LIU Zhiyong; LI Longxue; SHU Kun; LIU Guangnan; ZHANG Zuyun; YAN Weiyu; ZENG Zhijiang

doi:10.3969/j.issn.1006-267x.2020.01.046

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 1: 397 - 404

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.046

Regulating Effect of Natural Bee Bead and Bee Pollen on Immune Function of Mice

LI Zhen ,
LIU Zhiyong ,
LI Longxue ,
SHU Kun ,
LIU Guangnan ,
ZHANG Zuyun ,
YAN Weiyu ,
ZENG Zhijiang

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1. Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China;
2. Laboratory Animal Science and Technology Center, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China;
3. Agricultural and Rural Bureau of Shicheng County, Jiangxi Province, Shicheng 342700, China;
4. Sericultural and Apicultural Institute, Yunnan Academy of Agricultural Sciences, Mengzi 661100, China

Received date: 2019-07-02

Online published: 2020-01-19

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Abstract

The aim of this study was to study the regulating effect of natural bee bead and bee pollen on the immune function of mice. Kunming mice were used as experimental animals and divided into one blank control group and six experimental groups, and each group had 32 mice. The six experimental groups included three doses (low, middle and high doses) of natural bee bread groups and three doses (low, middle and high doses) of bee pollen groups. Referring to the human daily recommended feeding dosage[400 mg/(d·kg BW)] of bee pollen, low, middle and high doses were set as 80, 400 and 800 mg/(d·kg BW), respectively. After intragastric administration natural bee bread and bee pollen for 30 days, the mice were treated as follows:1) body weight, spleen and thymus indices were measured. 2) The natural killer (NK) cell activity was detected. 3) Concanavalin A (ConA)-induced transformation of mouse spleen lymphocytes experimental was performed. 4) Carbon clearance experiment was carried out. 5) Antibody producing cells were detected. The results showed as follows:the body weight among all groups in the same week age was no significant difference (P>0.05). The NK cell activity of mice in the middle dose bee bread group and the three bee pollen dose groups was significantly increased compared with the blank control group (P<0.05). There was no significant difference in the phagocytic index and the splenic lymphocyte proliferation capacity of mice in the six experimental groups compared with the blank control group (P>0.05). The thymus index of mice in low dose bee bread group was significantly increased compared with the three bee pollen dose groups (P<0.05). The number of hemolytic plaques of mice in the six experimental groups was significantly increased compared with the blank control group (P<0.05). Moreover, the number of hemolytic plaques of mice in the high dose bee bread group was significantly higher than that in the three bee pollen dose groups (P<0.05). It suggests that different doses of natural bee bread and bee pollen have no harmful effects on growth on mice, but both of them can increase the NK cell activity, enhance the humoral immune function, and the natural bee bread also can stimulate the development of thymus of mice. Overall, the effect of natural bee bread on regulating immune function is superior to bee pollen.

Key words： natural bee bread; bee pollen; mice; immune function

Cite this article

LI Zhen , LIU Zhiyong , LI Longxue , SHU Kun , LIU Guangnan , ZHANG Zuyun , YAN Weiyu , ZENG Zhijiang . Regulating Effect of Natural Bee Bead and Bee Pollen on Immune Function of Mice[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 397 -404 . DOI: 10.3969/j.issn.1006-267x.2020.01.046

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