Antibacterial Effects of Goose Oil Diglyceride in Vitro and Its Effects on Cecal Microflora Structure by Dextran Sulfate Sodium Induced Rats

  • XIE Yu'e ,
  • WANG Baowei ,
  • GE Wenhua ,
  • ZHANG Ming'ai ,
  • YUE Bin ,
  • LIU Guodong ,
  • LIU Ya'nan
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  • 1. Food Science and Engineering of Qingdao Agricultural University, Qingdao 266109, China;
    2. National Waterfowl Industrial Technology System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2019-07-01

  Online published: 2020-01-19

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Abstract

The aim of this study was to evaluate the bacteriostatic effects of goose oil diglyceride (GDG) in vitro and its effects on cecal microflora structure by dextran sulfate sodium (DSS) induced rats through bacteriostasis test in vitro and in vivo test in rats. Test 1 (bacteriostasis test in vitro):four kinds of bacteria of Escherichia coli, Salmonella, Staphylococcus aureus and Staphylococcus epidermidis were selected, the antibacterial activity of GDG was tested by perforating method, the antibacterial rate and growth curve of four kinds of bacteria were measured by ultraviolet spectrophotometer, and the morphological changes of GDG on four kinds of bacteria were observed by scanning electron microscope. Test 2 (in vivo test in rats):eighty-four 7-week-old male SD rats were randomly divided into 6 groups with 7 replicates per group and 2 rats perreplicate. Rats in the normal control group were free drank distilled water; the other 5 groups were drank 5% DSS aqueous solution, and they were divided into DSS model group, GDG low dose group, GDG medium dose group, GDG high dose group and enrofloxacin (ENR) group. Rats in each group were ad libitum and rats in the control group were free drank distilled water; during days 1 to 7 of the experiment, all the other 5 groups drank 5% DSS aqueous solution except the normal control group; during days 8 to 14 of the experiment, rats in DSS model group were given 10 mL/kg BW distilled water by gavage, rats in 3 GDG groups were given 1, 2 and 3 g/kg BW GDG by gavage, respectively, and rats in ENR group were given 10 mL/kg BW ENR (5 μg/mL) by gavage. After the end of the experiment, 16S rRNA high-throughput sequencing technology was used to determine the composition of cecal microflora of rats. The results showed as follows:1) different concentrations of GDG had significant inhibitory effects on growth of Escherichia coli, Salmonella, Staphylococcus aureus and Staphylococcus epidermidis (P<0.05), and the minimum inhibitory concentration (MIC) were 0.030, 0.040, 0.050 and 0.050 g/mL, respectively. 2) Scanning electron microscope image analysis showed that GDG could change the surface morphology of bacteria, destroy the overall structure of bacterial cells to inhibit the growth and reproduction of bacteria. 3) GDG could increase the species richness of cecal microflora in rats, optimized the flora structure of caecal microflora, and played a role in regulating intestinal microecological imbalance. It is concluded that GDG has significant antibacterial effects on Escherichia coli, Salmonella, Staphylococcus aureus and Staphylococcus epidermidis, and helpe DSS induce the structure of cecum flora in rats to return to normal.

Cite this article

XIE Yu'e , WANG Baowei , GE Wenhua , ZHANG Ming'ai , YUE Bin , LIU Guodong , LIU Ya'nan . Antibacterial Effects of Goose Oil Diglyceride in Vitro and Its Effects on Cecal Microflora Structure by Dextran Sulfate Sodium Induced Rats[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 405 -416 . DOI: 10.3969/j.issn.1006-267x.2020.01.047

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