SWINE NUTRITION AND FEED

Effects of Bacillus licheniformis on Intestinal Morphology, Intestinal Mucosal Antioxidant Capacity and Intestinal Immunity of Lipopolysaccharide-Stressed Piglets

  • HUAN Hailin ,
  • ZHANG Gan ,
  • XU Zhuhua ,
  • YAN Junshu ,
  • LIN Yong ,
  • XU Xiaoming ,
  • ZHOU Weiren
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  • 1. Institute of Animal Science, Jiangsu Academy of Agricultural Science, Nanjing 210014, China;
    2. Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, China;
    3. Taixing Ruitai Chemical Co., Ltd., Taizhou 225453, China

Received date: 2019-07-29

  Online published: 2020-02-21

Abstract

The aim of this study was to investigate the effects of Bacillus licheniformis on intestinal morphology, intestinal mucosal antioxidant capacity and intestinal immunity of lipopolysaccharide (LPS)-stressed piglets. The experiment used 90 heads of Sushan pigs of 35 days old and weighing about (10.0±0.5) kg, they were randomly divided into 3 groups (control group, LPS group and BL+LPS group) with 3 repetitions in each group and 10 repetitions each. The pigs in control group and LPS group were fed a basal diet, while the pigs in BL+LPS group were fed the basal diet supplemented with 500 mg/kg Bacillus licheniformis (1.0×1010 CFU/g). There was a pretrial period of 3-day followed by an experimental period of 21-day. Two piglets were selected from each group and injected intraperitoneally with 150 μg/kg BW LPS (LPS group and BL+LPS group) or an equivalent amount of sterile saline (control group) at day 21 of experimental period. After 24 h of injection, the piglets were slaughtered and sampled. The results showed as follows:1) compared with the control group, LPS stress significantly reduced the activities of diamine oxidase (DAO) in duodenum, nitric oxide synthetase (NOS) in jejunum and the content of nitric oxide (NO) in jejunum of weaned piglets (P<0.05), significantly increased the NO content and NOS activity in plasma (P<0.05), significantly decreased the villus height (VH) and VH/crypt depth (V/C) of jejunum and the V/C of ileum (P<0.05), significantly decreased the activities of glutathione peroxidase (GSH-Px) in duodenum, jejunum and ileum and superoxide dismutase (SOD) in ileum (P<0.05), and significantly reduce the counts of lymphocytes in jejunal epithelial cells and goblet cells in jejunal and ileal epithelial cells (P<0.05). In addition, LPS stress significantly decreased serum immunoglobulin (IgG) content and significantly increased interleukin-6 (IL-6) and interleukin-1β (IL-1β) contents in ileal mucosa (P<0.05). 2) Compared with the LPS group, adding Bacillus licheniformis in the diet significantly increased the activities of DAO and NOS in ileum (P<0.05), improved jejunum VH and V/C (P>0.05), significantly increased the activity of GSH-Px in jejunal mucosa (P<0.05), significantly decreased the content of MDA in duodenal mucosa (P<0.05), and significantly increased the content of IgG in serum of LPS-stressed piglets (P<0.05). The results indicate that LPS stress causes intestinal mucosal injury in piglets. Adding Bacillus licheniformis in the diet can improve the antioxidant capacity and immunity of intestinal mucosa of LPS-stressed piglets, promote the repair of intestinal morphology to a certain extent, and alleviate the intestinal damage caused by LPS stress.

Cite this article

HUAN Hailin , ZHANG Gan , XU Zhuhua , YAN Junshu , LIN Yong , XU Xiaoming , ZHOU Weiren . Effects of Bacillus licheniformis on Intestinal Morphology, Intestinal Mucosal Antioxidant Capacity and Intestinal Immunity of Lipopolysaccharide-Stressed Piglets[J]. Chinese Journal of Animal Nutrition, 2020 , 32(2) : 586 -595 . DOI: 10.3969/j.issn.1006-267x.2020.02.014

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